公开(公告)号：USD683044S1

公开(公告)日：2013-05-21

申请号：US29409184

申请日：2011-12-21

申请人： Matthias Klein ,  Gerd Lüdke ,  Andreas Boos

设计人： Matthias Klein ,  Gerd Lüdke ,  Andreas Boos

公开(公告)号：US09598721B2

公开(公告)日：2017-03-21

申请号：US13698216

申请日：2011-05-09

申请人： Matthias Klein ,  Gerd Lüdke ,  Andreas Boos

发明人： Matthias Klein ,  Gerd Lüdke ,  Andreas Boos

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1006

摘要： The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety of different sample types, such as different types of bodily samples relevant for the diagnosis of a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis. Preferably, these methods are universally applicable to a variety of sample types, are applicable as one-tube-processes, are 15 suitable for performance in a high-throughput setting, and are automatable.

公开(公告)号：US20130065223A1

公开(公告)日：2013-03-14

申请号：US13698216

申请日：2011-05-09

申请人： Matthias Klein ,  Gerd Lüdke ,  Andreas Boos

发明人： Matthias Klein ,  Gerd Lüdke ,  Andreas Boos

IPC分类号： C12N1/06 ,  C12N1/00 ,  C12Q1/68 ,  C12N9/50 ,  C12N9/56

CPC分类号： C12Q1/6806 ,  C12N15/1006

摘要： The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis. Preferably, these methods are universally applicable to a variety of sample types, are applicable as one-tube-processes, are 15 suitable for performance in a high-throughput setting, and are automatable.

摘要翻译： 本发明提供了一种普遍适用的裂解缓冲液，其包含离液序列5剂，还原剂和适用于处理各种不同样品类型的蛋白水解酶，例如与呼吸系统疾病诊断相关的不同类型的身体样品。 此外，本发明提供了离液剂，还原剂和蛋白水解酶用于裂解广谱的身体样品的用途。 此外，本发明提供了一种通常适用于各种不同类型的身体样品的裂解的身体样品的处理方法。 此外，本发明提供了用于分析身体样品或用于检测身体样品中病原体的存在的方法，优选用于诊断呼吸系统疾病如肺炎或结核病。 优选地，这些方法通常适用于各种样品类型，适用于单管工艺，适用于高通量设置中的性能，并且可自动化。

公开(公告)号：US20130130267A1

公开(公告)日：2013-05-23

申请号：US13698805

申请日：2011-05-19

申请人： Gerd Lüdke ,  Andreas Boos ,  Hassan Motejadded ,  Johannes Bacher

发明人： Gerd Lüdke ,  Andreas Boos ,  Hassan Motejadded ,  Johannes Bacher

IPC分类号： B01L7/00

CPC分类号： B01L7/52 ,  B01L3/502 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/049

摘要： The present invention provides a reaction vessel (20) for a PCR device. The reaction vessel (20) comprises a sample vial (32) defining a reaction chamber (33) for performing PCR and a storage vessel (62) defining a storage chamber (63) for optical detection. The reaction chamber (33) is in fluid communication with a liquid supply port (34) for supplying a liquid sample containing at least one target DNA to the reaction chamber (33). The reaction chamber (33) and the storage chamber (63) are in fluid communication via a spacer element (42) and a porous membrane (51) for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element (42) extends into the reaction chamber (33), but does not reach the bottom thereof. The upper end of the spacer element (42) is located in proximity of the porous membrane (51), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane (51) allows air as well as liquid to pass therethrough. In the wet state the porous membrane (51) still allows the passage of liquid therethrough, but not of air, such that during a PCR the liquid sample remains in the reaction chamber (33) and after the PCR the reaction vessel (20) is configured to force the liquid sample via the spacer element (42) to the porous membrane (51) for hybridization and detection of the at least one target DNA in the liquid sample. Moreover, a PCR device comprising such a reaction vessel (20) as well as a method for performing PCR are described.

摘要翻译： 本发明提供一种用于PCR装置的反应容器（20）。 反应容器（20）包括限定用于进行PCR的反应室（33）的样品瓶（32）和限定用于光学检测的储存室（63）的储存容器（62）。 反应室（33）与液体供应口（34）流体连通，用于向反应室（33）供应含有至少一种目标DNA的液体样品。 反应室（33）和储存室（63）通过间隔元件（42）和多孔膜（51）流体连通，用于将液体样品中的至少一个目标DNA杂交到特定的固定化杂交探针上。 间隔元件（42）的下端延伸到反应室（33）中，但不到达其底部。 间隔元件（42）的上端位于多孔膜（51）附近，多孔膜（51）由干燥状态和潮湿状态下具有不同物理性质的材料制成。 在干燥状态下，多孔膜（51）允许空气以及液体通过。 在湿状态下，多孔膜（51）仍然允许液体通过其中而不是空气通过，使得在PCR期间液体样品保留在反应室（33）中，并且在PCR之后，反应容器（20）为 被配置为将液体样品经由间隔元件（42）强制到多孔膜（51），用于杂交和检测液体样品中的至少一种靶DNA。 此外，描述包含这种反应容器（20）的PCR装置以及进行PCR的方法。

公开(公告)号：US09963670B2

公开(公告)日：2018-05-08

申请号：US14232391

申请日：2011-07-27

申请人： Andreas Boos ,  Gerd Lüdke ,  Johannes Bacher

发明人： Andreas Boos ,  Gerd Lüdke ,  Johannes Bacher

IPC分类号： C12M1/00 ,  G01N1/28 ,  G01N1/38 ,  G01N1/44 ,  C12N1/06 ,  G01N35/02 ,  G01N35/04 ,  G01N35/00

CPC分类号： C12M47/06 ,  C12N1/066 ,  G01N1/286 ,  G01N1/38 ,  G01N1/44 ,  G01N35/025 ,  G01N2035/00504 ,  G01N2035/00524 ,  G01N2035/0441

摘要： The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample. The apparatus comprises at least one rotation disc (31), at least one vial holder (90) which is configured to receive a vial (100), wherein the vial holder (90) is arranged on the disc (31), at least one driving device (20) which is configured to rotate the disc (31) and the vial holder (90), at least one heating device (60) which is configured to heat the sample to a determined incubation temperature, and—at least one control device (70) which is configured to control the driving device (20) and/or the heating device (60) by means of a timing and/or step control, and/or—at least one transmitting device (80) for inductive coupling for energy and signal transmission, which is configured to transmit the energy for heating to the heating device (60), and/or—wherein the driving device (20) is configured to rotate the disc (31) in a first direction (A1) and/or with a first speed, and to rotate the vial holder (90) in a second direction (A3) and/or with a second speed. The apparatus and the method are adapted for an (automated) lysis procedure, wherein the lysis can be carried out in a safe, efficient and effective manner.

公开(公告)号：US09592511B2

公开(公告)日：2017-03-14

申请号：US13698805

申请日：2011-05-19

申请人： Gerd Lüdke ,  Andreas Boos ,  Hassan Motejadded ,  Johannes Bacher

发明人： Gerd Lüdke ,  Andreas Boos ,  Hassan Motejadded ,  Johannes Bacher

IPC分类号： B01L7/00 ,  B01L3/00

CPC分类号： B01L7/52 ,  B01L3/502 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/049

摘要： The present invention provides a reaction vessel (20) for a PCR device. The reaction vessel (20) comprises a sample vial (32) defining a reaction chamber (33) for performing PCR and a storage vessel (62) defining a storage chamber (63) for optical detection. The reaction chamber (33) is in fluid communication with a liquid supply port (34) for supplying a liquid sample containing at least one target DNA to the reaction chamber (33). The reaction chamber (33) and the storage chamber (63) are in fluid communication via a spacer element (42) and a porous membrane (51) for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element (42) extends into the reaction chamber (33), but does not reach the bottom thereof. The upper end of the spacer element (42) is located in proximity of the porous membrane (51), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane (51) allows air as well as liquid to pass therethrough. In the wet state the porous membrane (51) still allows the passage of liquid therethrough, but not of air, such that during a PCR the liquid sample remains in the reaction chamber (33) and after the PCR the reaction vessel (20) is configured to force the liquid sample via the spacer element (42) to the porous membrane (51) for hybridization and detection of the at least one target DNA in the liquid sample. Moreover, a PCR device comprising such a reaction vessel (20) as well as a method for performing PCR are described.

摘要翻译： 本发明提供一种用于PCR装置的反应容器（20）。 反应容器（20）包括限定用于进行PCR的反应室（33）的样品瓶（32）和限定用于光学检测的储存室（63）的储存容器（62）。 反应室（33）与液体供应口（34）流体连通，用于向反应室（33）供应含有至少一种目标DNA的液体样品。 反应室（33）和储存室（63）通过间隔元件（42）和多孔膜（51）流体连通，用于将液体样品中的至少一个目标DNA杂交到特定的固定化杂交探针上。 间隔元件（42）的下端延伸到反应室（33）中，但不到达其底部。 间隔元件（42）的上端位于多孔膜（51）附近，多孔膜（51）由干燥状态和潮湿状态下具有不同物理性质的材料制成。 在干燥状态下，多孔膜（51）允许空气以及液体通过。 在湿状态下，多孔膜（51）仍然允许液体通过其中而不是空气通过，使得在PCR期间液体样品保留在反应室（33）中，并且在PCR之后，反应容器（20）为 被配置为将液体样品经由间隔元件（42）强制到多孔膜（51），用于杂交和检测液体样品中的至少一种靶DNA。 此外，描述包含这种反应容器（20）的PCR装置以及进行PCR的方法。

公开(公告)号：US20140242678A1

公开(公告)日：2014-08-28

申请号：US14232391

申请日：2011-07-27

申请人： Andreas Boos ,  Gerd Lüdke ,  Johannes Bacher

发明人： Andreas Boos ,  Gerd Lüdke ,  Johannes Bacher

IPC分类号： C12M1/00 ,  C12N1/06

CPC分类号： C12M47/06 ,  C12N1/066 ,  G01N1/286 ,  G01N1/38 ,  G01N1/44 ,  G01N35/025 ,  G01N2035/00504 ,  G01N2035/00524 ,  G01N2035/0441

摘要： The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample. The apparatus comprises at least one rotation disc (31), at least one vial holder (90) which is configured to receive a vial (100), wherein the vial holder (90) is arranged on the disc (31), at least one driving device (20) which is configured to rotate the disc (31) and the vial holder (90), at least one heating device (60) which is configured to heat the sample to a determined incubation temperature, and—at least one control device (70) which is configured to control the driving device (20) and/or the heating device (60) by means of a timing and/or step control, and/or—at least one transmitting device (80) for inductive coupling for energy and signal transmission, which is configured to transmit the energy for heating to the heating device (60), and/or—wherein the driving device (20) is configured to rotate the disc (31) in a first direction (A1) and/or with a first speed, and to rotate the vial holder (90) in a second direction (A3) and/or with a second speed. The apparatus and the method are adapted for an (automated) lysis procedure, wherein the lysis can be carried out in a safe, efficient and effective manner.

摘要翻译： 本发明提供了一种用于裂解程序的装置和方法，特别是用于样品，特别是生物样品的自动和/或受控裂解方法。 该设备包括至少一个旋转圆盘（31），至少一个小瓶支架（90），其构造成接收小瓶（100），其中小瓶支架（90）布置在圆盘（31）上，至少一个 驱动装置（20），其构造成使盘（31）和小瓶保持器（90）旋转;至少一个加热装置（60），其被配置为将样品加热至确定的培养温度;以及 - 至少一个控制 设备（70），其被配置为通过定时和/或步进控制来控制驱动装置（20）和/或加热装置（60），和/或至少一个用于感应耦合的发送装置（80） 用于能量和信号传输，其被配置为将用于加热的能量传输到加热装置（60），和/或 - 其中所述驱动装置（20）被配置为沿第一方向（A1）旋转所述盘（31） 和/或以第一速度旋转，并且在第二方向（A3）和/或以第二速度旋转小瓶保持器（90）。 该装置和方法适用于（自动）裂解程序，其中裂解可以以安全，有效和有效的方式进行。