公开(公告)号：US5037751A

公开(公告)日：1991-08-06

申请号：US405553

申请日：1989-09-11

申请人： Mauro A. Bertola ,  Arthur F. Marx ,  Hein S. Koger ,  Wilhelmus J. Quax ,  Cornelis J. van der Laken ,  Gareth T. Phillips ,  Brian W. Robertson ,  Peter D. Watts

发明人： Mauro A. Bertola ,  Arthur F. Marx ,  Hein S. Koger ,  Wilhelmus J. Quax ,  Cornelis J. van der Laken ,  Gareth T. Phillips ,  Brian W. Robertson ,  Peter D. Watts

IPC分类号： C12N15/09 ,  A61K31/19 ,  A61K31/33 ,  A61P25/04 ,  A61P29/00 ,  C07C57/30 ,  C07D209/46 ,  C07H21/04 ,  C12N1/20 ,  C12N1/21 ,  C12N9/18 ,  C12N15/00 ,  C12P7/40 ,  C12P17/00 ,  C12P41/00 ,  C12R1/06 ,  C12R1/10 ,  C12R1/125 ,  C12R1/19 ,  C12R1/38 ,  C12R1/385 ,  C12R1/39 ,  C12R1/40 ,  C12R1/465 ,  C12R1/785

CPC分类号： C12P41/005 ,  C12N9/18 ,  C12P17/00 ,  C12P7/40 ,  Y10S435/822 ,  Y10S435/83 ,  Y10S435/836 ,  Y10S435/839 ,  Y10S435/849 ,  Y10S435/874 ,  Y10S435/886

摘要： A process for the preparation of a pharmaceutically active compound in a stereospecific form of the formula ##STR1## or a pharmaceutically acceptable salt or ester thereof, like an alkali metal salt or an alkaline earth metal salt or a pivaloyl ester, wherein R.sub.1 represents an optionally substituted aryl group such as a phenyl or naphthyl group optionally included in a heterocyclic ring system, which is optionally substituted, or represents a heteroaromatic ring system containing in addition to carbon atoms one or more atoms selected from nitrogen, sulphur and oxygen, this ring system being optionally substituted, which comprises subjecting a compound of the formula ##STR2## wherein R.sub.2 is an ester residue and preferably an alkyl group optionally substituted, to the action of a micro-organism having the ability for stereoselective hydrolysis of compound (II) into compound (I), having at least 80% by weight the S-configuration, and if desired converting compound (I) into the pharmaceutically acceptable salt or ester thereof.

公开(公告)号：US4990447A

公开(公告)日：1991-02-05

申请号：US317818

申请日：1989-03-01

申请人： Boudewijn W. Konig ,  Michiel N. Hamers ,  Cornelis J. van der Laken

发明人： Boudewijn W. Konig ,  Michiel N. Hamers ,  Cornelis J. van der Laken

IPC分类号： C07K14/765 ,  C12N15/14

CPC分类号： C07K14/765

摘要： Recombinantly produced serum albumin is purified in a series of steps, initially employing an alkaline precipitation, optionally followed by incubation with an anion exchange absorbent, followed by affinity chromatography employing a hydrophobic solid phase and using a water-soluble lipid anion as desorbens in the aqueous phase.

摘要翻译： 重组产生的血清白蛋白在一系列步骤中纯化，最初采用碱性沉淀，任选地随后与阴离子交换吸收剂一起温育，然后使用疏水性固相进行亲和层析，并使用水溶性脂质阴离子作为水溶液中的解吸 相。

公开(公告)号：US5238831A

公开(公告)日：1993-08-24

申请号：US884465

申请日：1992-05-14

申请人： Johanna H. G. M. Mutsaers ,  Cornelis J. van der Laken ,  Cornelis P. Broekhuizen ,  Wilhemus J. Quax

发明人： Johanna H. G. M. Mutsaers ,  Cornelis J. van der Laken ,  Cornelis P. Broekhuizen ,  Wilhemus J. Quax

IPC分类号： C12N9/18 ,  C12P7/40

CPC分类号： C12P7/40 ,  C12N9/18

摘要： Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.

摘要翻译： 羧基酯酶由几种化合物如萘普生或双氯芬酸灭活。 通过取代或修饰羧基酯酶的某些碱性残基，该酶在施用期间显示改善的稳定性。 以这种方式，即使在高底物浓度下也可以在工业规模下进行立体特异性水解反应。