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公开(公告)号：US5286646A
公开(公告)日：1994-02-15
申请号：US763867
申请日：1991-09-20
摘要： The invention presents a method for the culturing of mammalian cells. This method involves the use of a bioreactor, which contains a sample of mammalian cells in a culture medium containing large molecules. Positioned inside the bioreactor is a semipermeable membrane which defines a space separated from the bioreactor by the semipermeable membrane. A nutrient medium flows through this separated space and, via virtue of the semipermeable nature of the separating membrane, nutrient pass therethrough into the culture medium, while cellular waste products pass into the separated space. The semipermeable membrane is selected so that the cells and large molecules, such as proteinaceous materials, cannot pass through the membrane, but remain in the bioreactor.
摘要翻译： 本发明提出了哺乳动物细胞的培养方法。 该方法涉及使用含有大分子的培养基中的哺乳动物细胞样品的生物反应器。 定位在生物反应器内的是半透膜，其限定了通过半透膜与生物反应器分离的空间。 营养培养基流过该分离的空间，并且由于分离膜的半透性，营养物通过其进入培养基，而细胞废物进入分离的空间。 选择半透膜，使得细胞和大分子（例如蛋白质材料）不能通过膜，而是保留在生物反应器中。
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公开(公告)号：US4840905A
公开(公告)日：1989-06-20
申请号：US106115
申请日：1987-10-07
CPC分类号： B01F7/00641 , B01F7/16 , C12M27/02
摘要： A process for culturing biological material capable of multiplication, especially cells securely adhering to microcarriers, employs a bioreactor having a vessel for a culture medium having means for controlling the environmental conditions in the culture medium and a stirring device for the homogeneous distribution of the cells in the culture medium which has a rotary drive and a rotation axle running in the interior of the vessel, wherein the stirring device has at least one flat stirrer blade fixed on to the rotation axle and inclined to the rotation axle.
摘要翻译： 用于培养能够增殖的生物材料，特别是牢固粘附于微载体的细胞的方法，采用具有培养基容器的生物反应器，其具有用于控制培养基中的环境条件的装置和用于均匀分布细胞的搅拌装置 所述培养介质具有在所述容器的内部运行的旋转驱动和旋转轴，其中所述搅拌装置具有固定在所述旋转轴上且与所述旋转轴倾斜的至少一个平板搅拌器叶片。
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公开(公告)号：US4385120A
公开(公告)日：1983-05-24
申请号：US284115
申请日：1981-07-16
申请人： Anthony Atkinson , Michael J. Comer
发明人： Anthony Atkinson , Michael J. Comer
CPC分类号： C12R1/07 , C12N9/12 , Y10S435/832
摘要： A thermostable glycerokinase enzyme useful in the detection and estimation of glycerol derivatives has a half-life in excess of 1 hour at 55.degree. C. at a protein concentration of less than 2 mg/ml and a pH of 7.8 .+-.0.5 in the absence of any substrate. The enzyme is produced by culturing at least one micro-organism which is capable of growth at a temperature of at least 50.degree. C. in a culture medium in which it will produce said enzyme, disrupting the resulting cells of the micro-organism to release the enzyme and separating the enzyme from the cell debris. The medium normally contains at least 0.1% glycerol but certain strains of micro-organism have been found to be capable of producing thermostable glycerokinase enzyme even in the absence of glycerol. The micro-organism is preferably a Bacillus organism, especially of the stearothermophilus species.
摘要翻译： 用于检测和估计甘油衍生物的热稳定甘油三酯酶在55℃下的半衰期超过1小时，蛋白质浓度小于2mg / ml，pH为7.8 +/- 0.5， 没有任何底物。 该酶通过培养至少一种微生物产生，所述微生物能够在其将产生所述酶的培养基中在至少50℃的温度下生长，破坏所得到的微生物细胞释放 酶和酶从细胞碎片分离。 该培养基通常含有至少0.1％的甘油，但是即使在不存在甘油的情况下，已经发现某些微生物菌株能够产生热稳定的甘油三酯酶。 微生物优选是芽孢杆菌属生物，特别是嗜热脂肪芽孢杆菌属物种。
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公开(公告)号：US4746609A
公开(公告)日：1988-05-24
申请号：US742800
申请日：1985-06-10
申请人： Bryan Bolton , Michael J. Comer , Christoph Kessler , Georg Nesch
发明人： Bryan Bolton , Michael J. Comer , Christoph Kessler , Georg Nesch
CPC分类号： C12N9/22 , Y10S435/824
摘要： The present invention teaches restriction endonuclease Asp 718 which cleaves DNA in the palindromic recognition sequence at the sites indicated by the arrow: ##STR1## The endonuclease, obtained from Achromobacter species 718, DSM 2969, is useful in obtaining DNA fragments, e.g., for determining nucleotide sequences and other types of analysis of DNA.
摘要翻译： 本发明教导了限制性内切核酸酶Asp 718，其在由箭头指示的位点处的回文识别序列中切割DNA：从无色杆菌属物种718（DSM 2969）获得的核酸内切酶可用于获得DNA片段，例如用于确定 核苷酸序列和其他类型的DNA分析。
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公开(公告)号：US5118603A
公开(公告)日：1992-06-02
申请号：US363887
申请日：1989-06-01
CPC分类号： A61L2/0088
摘要： A method of inactivating the biological activity of natural or recombinant DNA in a biomass, by adding a percarboxylic acid containing 1 to 3 carbon atoms, one of its salts, an alkali metal peroxide, or an alkali metal peroxomonosulfate and subsequently heating the mixture to 60.degree. to 100.degree. C.
摘要翻译： PCT No.PCT / EP88 / 00592 Sec。 371日期：1989年6月1日 102（e）日期1989年6月1日PCT Filed 1988年7月4日PCT公布。 出版物WO89 / 03226 日本1989年4月20日。一种通过加入含有1至3个碳原子的过羧酸，其盐，其中一种盐，碱金属过氧化物或碱金属过碘酸盐使生物质中天然或重组DNA的生物活性失活的方法 随后将混合物加热至60℃至100℃。
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公开(公告)号：US4342827A
公开(公告)日：1982-08-03
申请号：US41481
申请日：1979-05-22
CPC分类号： C12N9/0006 , C12Q1/32 , Y10S435/832
摘要： Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40.degree.-65.degree. C. and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molcular weight of 240,000.+-.30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30.degree. C. by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids.The enzymes may be used for assay of serum triglycerides by conventional assay methods, but preferably by the nictotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer.
摘要翻译： 具有非常好的热稳定性的甘油脱氢酶通过培养嗜热脂肪芽孢杆菌的新菌株而产生。 描述用于导出和鉴定合适菌株的程序。 菌株在常规培养基中生长，优选在40-65℃和pH 5至8下含有0.05-4.0％，特别是0.1-10.0重量％的甘油或甘油类似物。通过常规方法分离酶 细胞破碎和分离技术，并且通常具有240,000 +/- 30,000的微球重量，由四个相似的亚单位组成，并且通过所述修饰的测定法在30℃下比例活性大于5单位/ mg蛋白质。 它们可以作为水溶液或冷冻干燥的固体储存。 酶可以用于通过常规测定方法测定血清甘油三酸酯，但优选通过在7至8.8的pH下的烟酰胺腺嘌呤二核苷酸分光光度测定法。 pH优选由胺，特别是三乙醇胺/ HCl缓冲液控制。
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