利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

34 条记录 (耗时 0.02 秒)

    Thermostable glycerokinases and process for its production
    1.
    发明授权
    Thermostable glycerokinases and process for its production 失效
    热稳定性甘油酯酶及其生产工艺

    公开(公告)号：US4385120A

    公开(公告)日：1983-05-24

    申请号：US284115

    申请日：1981-07-16

    申请人： Anthony Atkinson ,  Michael J. Comer

    发明人： Anthony Atkinson ,  Michael J. Comer

    IPC分类号： C12N9/12 ,  C12R1/07 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12R1/07 ,  C12N9/12 ,  Y10S435/832

    摘要： A thermostable glycerokinase enzyme useful in the detection and estimation of glycerol derivatives has a half-life in excess of 1 hour at 55.degree. C. at a protein concentration of less than 2 mg/ml and a pH of 7.8 .+-.0.5 in the absence of any substrate. The enzyme is produced by culturing at least one micro-organism which is capable of growth at a temperature of at least 50.degree. C. in a culture medium in which it will produce said enzyme, disrupting the resulting cells of the micro-organism to release the enzyme and separating the enzyme from the cell debris. The medium normally contains at least 0.1% glycerol but certain strains of micro-organism have been found to be capable of producing thermostable glycerokinase enzyme even in the absence of glycerol. The micro-organism is preferably a Bacillus organism, especially of the stearothermophilus species.

    摘要翻译： 用于检测和估计甘油衍生物的热稳定甘油三酯酶在55℃下的半衰期超过1小时，蛋白质浓度小于2mg / ml，pH为7.8 +/- 0.5， 没有任何底物。 该酶通过培养至少一种微生物产生，所述微生物能够在其将产生所述酶的培养基中在至少50℃的温度下生长，破坏所得到的微生物细胞释放 酶和酶从细胞碎片分离。 该培养基通常含有至少0.1％的甘油，但是即使在不存在甘油的情况下，已经发现某些微生物菌株能够产生热稳定的甘油三酯酶。 微生物优选是芽孢杆菌属生物，特别是嗜热脂肪芽孢杆菌属物种。

    Production of enzymes
    2.
    发明授权
    Production of enzymes 失效
    生产酶

    公开(公告)号：US4342827A

    公开(公告)日：1982-08-03

    申请号：US41481

    申请日：1979-05-22

    申请人： Anthony Atkinson ,  Christopher J. Bruton ,  Michael J. Comer ,  Richard J. Sharp

    发明人： Anthony Atkinson ,  Christopher J. Bruton ,  Michael J. Comer ,  Richard J. Sharp

    IPC分类号： C12N1/20 ,  C12N9/04 ,  C12Q1/32 ,  C12R1/07 ,  C12N15/00

    CPC分类号： C12N9/0006 ,  C12Q1/32 ,  Y10S435/832

    摘要： Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40.degree.-65.degree. C. and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molcular weight of 240,000.+-.30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30.degree. C. by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids.The enzymes may be used for assay of serum triglycerides by conventional assay methods, but preferably by the nictotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer.

    摘要翻译： 具有非常好的热稳定性的甘油脱氢酶通过培养嗜热脂肪芽孢杆菌的新菌株而产生。 描述用于导出和鉴定合适菌株的程序。 菌株在常规培养基中生长，优选在40-65℃和pH 5至8下含有0.05-4.0％，特别是0.1-10.0重量％的甘油或甘油类似物。通过常规方法分离酶 细胞破碎和分离技术，并且通常具有240,000 +/- 30,000的微球重量，由四个相似的亚单位组成，并且通过所述修饰的测定法在30℃下比例活性大于5单位/ mg蛋白质。 它们可以作为水溶液或冷冻干燥的固体储存。 酶可以用于通过常规测定方法测定血清甘油三酸酯，但优选通过在7至8.8的pH下的烟酰胺腺嘌呤二核苷酸分光光度测定法。 pH优选由胺，特别是三乙醇胺/ HCl缓冲液控制。

    Media for affinity chromatography
    4.
    发明授权
    Media for affinity chromatography 失效
    介质用于亲和层析

    公开(公告)号：US4546161A

    公开(公告)日：1985-10-08

    申请号：US545546

    申请日：1983-10-26

    申请人： Michael J. Harvey ,  Anthony Atkinson

    发明人： Michael J. Harvey ,  Anthony Atkinson

    IPC分类号： B01J20/281 ,  A61K38/00 ,  B01D15/08 ,  B01J20/32 ,  C07K1/22 ,  C07K14/76 ,  C09B62/04 ,  C12N9/00 ,  C12N9/04 ,  C12N9/12 ,  C12N9/18 ,  C12N9/72 ,  G01N30/88 ,  G01N33/53 ,  C08B31/00 ,  C08B37/02

    CPC分类号： C12N9/0006 ,  B01D15/3804 ,  B01D15/3809 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3255 ,  C07K1/22 ,  C07K14/76 ,  C09B62/04 ,  C12N9/00 ,  C12N9/12 ,  C12N9/18 ,  C12N9/6462 ,  C12Y304/21073

    摘要： Affinity chromatography media are prepared by reacting mono or di-chloro triazine dyes with a solid support matrix containing free hydroxy or amino groups in the presence of an alkali metal hydroxide and an alkali metal salt. In the absence of cyanogen bromide activation, the linkage to the support is entirely via the triazine ring giving high and specific protein-binding capacity. Suitable support matrices include polymers and copolymers of agarose, dextrose, dextran and acrylamide with agarose being particularly preferred. Dye binding levels are much higher than those obtained in the presence of carbonates or bicarbonates.Mono-chlorotriazinyl dyes are bound at a pH which is preferably above 9.5. The reaction time is about 40-60 hours at room temperature, however this may be reduced if the reaction temperature is increased. Dichlorotriazinyl dyes are bound at pH 8 to 12.5 in a few hours at room temperature.The media may be used for the efficient and often highly specific purification of a wide variety of proteins, including enzymes and blood proteins.

    摘要翻译： 通过在碱金属氢氧化物和碱金属盐的存在下，使单或二氯三嗪染料与含有游离羟基或氨基的固体支持基质反应来制备亲和层析介质。 在没有溴化氰活化的情况下，与载体的连接完全是通过三嗪环提供高和特异性的蛋白结合能力。 合适的载体基质包括琼脂糖，葡萄糖，葡聚糖和丙烯酰胺与琼脂糖的聚合物和共聚物是特别优选的。 染料结合水平远高于在碳酸盐或碳酸氢盐存在下获得的结合水平。 单氯三嗪染料在优选高于9.5的pH下结合。 反应时间在室温下约为40-60小时，但如果反应温度升高，则反应时间可能会降低。 二氯三嗪染料在室温下几小时内在pH 8至12.5下结合。 培养基可用于多种蛋白质（包括酶和血液蛋白质）的有效且经常高度特异性的纯化。

    Method for Culturing Microorganisms
    6.
    发明申请
    Method for Culturing Microorganisms 审中-公开
    培养微生物的方法

    公开(公告)号：US20080305536A1

    公开(公告)日：2008-12-11

    申请号：US12066526

    申请日：2006-10-05

    申请人： Anthony Atkinson ,  Roger Cripps ,  Kirstin Eley

    发明人： Anthony Atkinson ,  Roger Cripps ,  Kirstin Eley

    IPC分类号： C12N1/36

    CPC分类号： C12N1/36 ,  C12P7/065 ,  Y02E50/17

    摘要： The present invention is a method for the production of thermophilic microorganisms suitable for the production of ethanol, comprising: (i) culturing a thermophilic microorganism under aerobic or anaerobic conditions in a suitable culture media; and (ii) incorporating amounts of ethanol into the culture media to induce ethanol tolerance.

    摘要翻译： 本发明是一种生产适用于生产乙醇的嗜热微生物的方法，包括：（i）在有氧或无氧条件下在合适的培养基中培养嗜热微生物; 和（ii）将乙醇的量加入到培养基中以诱导乙醇耐受。