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公开(公告)号：US20090054267A1
公开(公告)日：2009-02-26
申请号：US12216062
申请日：2008-06-27
IPC分类号： C40B60/12
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US07407814B2
公开(公告)日：2008-08-05
申请号：US09927779
申请日：2001-08-09
IPC分类号： G01N33/543
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US07989198B2
公开(公告)日：2011-08-02
申请号：US12216062
申请日：2008-06-27
IPC分类号： C12M3/00
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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4.发明授权公开(公告)号：US07375321B2
公开(公告)日：2008-05-20
申请号：US10502551
申请日：2003-05-07
IPC分类号： G01N13/16
CPC分类号： G01N33/54373
摘要： A bioNEMS device comprises a piezoresistive cantilever having flexing legs of which attach the cantilever to a support and a biofunctionalized portion at the tip. A bias current applied to the legs is limited by a maximal acceptable temperature increase at the biofunctionalized tip. The length of the cantilever has a magnitude chosen to minimize background Johnson noise. A catalyzed receptor on the device binds to a ligand whose binding rate coefficient is enhanced. The catalyst lowers the receptor-ligand binding activation energy and is designed by forced evolution to preferentially bind with the ligand. A carrier signal is injected by a magnetic film disposed on the cantilever which is electromagnetically coupled to a source of the carrier signal. A plurality of NEMS fluidicly coupled transducers generate a plurality of output signals from which a collective output signal is derived, either by averaging or thresholding. The NEMS devices are disposed in microfluidic flow channels and fabricated in a membrane. A linking molecule is attached to the tip of the transducer and a fluffball attached to the linking molecule to increase damping.
摘要翻译： bioNEMS装置包括压阻悬臂，其具有将悬臂连接到支撑件上的弯曲腿和在尖端的生物功能化部分。 施加到腿上的偏置电流受生物功能化尖端的最大可接受的温度升高的限制。 悬臂的长度具有选择的最小化背景约翰逊噪声的大小。 器件上的催化受体与结合速率系数增强的配体结合。 催化剂降低受体 - 配体结合活化能，并通过强制进化来优先与配体结合而设计。 载体信号由布置在悬臂上的磁膜注入，该磁膜电磁耦合到载波信号源。 多个NEMS流体耦合的换能器产生多个输出信号，通过平均或阈值从该输出信号导出集体输出信号。 NEMS装置设置在微流体流动通道中并制成膜。 连接分子连接到换能器的尖端，并且连接到连接分子上的绒毛增加阻尼。
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公开(公告)号：US08329452B2
公开(公告)日：2012-12-11
申请号：US13183336
申请日：2011-07-14
IPC分类号： C12M1/34
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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6.发明授权Method and apparatus for providing signal analysis of a BioNEMS resonator or transducer 有权用于提供BioNEMS谐振器或换能器的信号分析的方法和装置公开(公告)号：US07330795B2
公开(公告)日：2008-02-12
申请号：US10502466
申请日：2003-05-07
IPC分类号： G01N33/50
CPC分类号： G01N33/54373
摘要： An outputs signal, v(t), is generated from a bioNEMs transducer and mixed with a reference signal and then filtered to generate a correlator output, r(t). The correlator output is detected to generate a signal u(t) and then determined whether the signal u(t) satisfies a predetermined threshold. If qualified, it is then decided whether the signal u(t) represents a predetermined type of interaction between a free ligand in a fluid in which the NEMS device is immersed and a receptor attached to the transducer. The threshold is the Neyman-Pearson criterion based on a predetermined probability of false detection, Pfa. The interaction may be binding of a free ligand to the receptor or releasing a bound ligand from the receptor by competitive binding with the free ligand. The step of detecting comprises detecting the envelope of the signal, r(t).
摘要翻译： 输出信号v（t）由bioNEMs传感器产生并与参考信号混合，然后被滤波以产生相关器输出r（t）。 检测相关器输出以产生信号u（t），然后确定信号u（t）是否满足预定阈值。 如果合格，则判定信号u（t）是否表示在其中浸入NEMS装置的流体中的游离配体与附接到换能器的受体之间的预定类型的相互作用。 阈值是基于预先确定的错误检测概率的Neyman-Pearson标准Pfa。 相互作用可以是游离配体与受体的结合或通过与游离配体的竞争性结合从受体释放结合的配体。 检测步骤包括检测信号的包络r（t）。
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7.发明授权公开(公告)号：US09116128B2
公开(公告)日：2015-08-25
申请号：US13346484
申请日：2012-01-09
IPC分类号： G01N33/547 , G01N33/553 , G01N33/543 , G01N21/77
CPC分类号： G01N21/7746 , G01N33/54353 , G01N2021/7789
摘要： Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
摘要翻译： 微腔谐振传感器具有使用点击化学功能化的外表面，例如涉及炔官能团和叠氮官能团的环加成反应。 第一聚合物连接元件与微腔的外表面结合并具有叠氮化物官能团，其结合环加成反应的结果与第二聚合物连接元素的炔官能团结合。 用于感测靶分子的功能化元件如抗体，抗原或蛋白质被结合到第二连接元件。
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公开(公告)号：US08507204B2
公开(公告)日：2013-08-13
申请号：US13363022
申请日：2012-01-31
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译： 本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。 在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。 在将样品与探针接触后，提供形成系留聚合物的标记单体。 触发的探针和自熄单体可用于提供活性背景抑制。
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公开(公告)号：US20120120398A1
公开(公告)日：2012-05-17
申请号：US13361076
申请日：2012-01-30
IPC分类号： G01N21/00
CPC分类号： G01N21/7746 , G01N21/783 , G01N33/0037 , G01N33/0047 , G01N33/0049 , G01N33/0054 , G01N33/22 , G01N2021/773 , G01N2021/7789 , Y02A50/245 , Y02A50/246 , Y02A50/25
摘要： Micro-cavity gas or vapor sensors and gas or vapor detection methods. Optical energy is introduced into a resonant micro-cavity having a deformable coating such as a polymer. The coating swells or expands when it is exposed to or absorbs a gas or vapor, thereby changing the resonant wavelength of optical energy circulating within the micro-cavity/coating. Expansion or swelling of the coating may be reversible such that it contracts when gas or vapor diffuses from the coating. The coating deformation and/or a change of one or more optical properties of the optical energy circulating within the micro-cavity are used to detect the presence of the gas or vapor or molecules or particulates thereof
摘要翻译： 微腔气体或蒸气传感器以及气体或蒸气检测方法。 将光能引入具有可变形涂层如聚合物的共振微腔中。 当涂层暴露于或吸收气体或蒸气时，涂层膨胀或膨胀，从而改变在微腔/涂层内循环的光能的共振波长。 涂层的膨胀或膨胀可以是可逆的，使得当气体或蒸汽从涂层扩散时，其膨胀或膨胀。 使用在微腔内循环的光能的涂层变形和/或一种或多种光学特性的变化来检测气体或蒸汽或其分子或颗粒的存在
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10.发明申请公开(公告)号：US20120107177A1
公开(公告)日：2012-05-03
申请号：US13346484
申请日：2012-01-09
IPC分类号： G01N33/53
CPC分类号： G01N21/7746 , G01N33/54353 , G01N2021/7789
摘要： Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
摘要翻译： 微腔谐振传感器具有使用点击化学功能化的外表面，例如涉及炔官能团和叠氮官能团的环加成反应。 第一聚合物连接元件与微腔的外表面结合并具有叠氮化物官能团，其结合环加成反应的结果与第二聚合物连接元素的炔官能团结合。 用于感测靶分子的功能化元件如抗体，抗原或蛋白质被结合到第二连接元件。
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