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    Microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method
    1.
    发明申请
    Microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method 审中-公开
    基于微阵列的单核苷酸多态性，测序和基因表达测定方法

    公开(公告)号：US20060240443A1

    公开(公告)日：2006-10-26

    申请号：US11110630

    申请日：2005-04-20

    申请人： Michael Lodes ,  Dominic Suciu ,  Andy McShea

    发明人： Michael Lodes ,  Dominic Suciu ,  Andy McShea

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6874

    摘要： There is disclosed a microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method. Specifically, there is disclosed a method using a microarray device wherein a plurality of hybridized structures is formed by contacting the microarray under a hybridizing condition to a hybridizing solution comprising a plurality of tagged targets and a plurality of detection sequences. The detection sequences of each hybridized structure is extended using an extension-ligation solution and an extension-ligation condition. After extension, ligation of the extended sequence occurs to a probe if the terminal nucleotide of a probe is complementary to the hybridized tagged targets. Non-bound material is removed by using a washing solution and a washing method. The target nucleotide and the target sequence of the tagged targets is determined by which probe is ligated to the detection sequences.

    摘要翻译： 公开了基于微阵列的单核苷酸多态性，测序和基因表达测定方法。 具体地，公开了使用微阵列装置的方法，其中通过在杂交条件下将微阵列与包含多个标记的靶和多个检测序列的杂交溶液接触而形成多个杂交结构。 使用延伸连接溶液和延伸连接条件扩展每个杂交结构的检测序列。 延伸后，如果探针的末端核苷酸与杂交标记的靶标互补，则延伸序列的连接发生在探针上。 通过使用洗涤溶液和洗涤方法除去未结合的物质。 通过将哪个探针连接到检测序列来确定标记的靶标的靶核苷酸和靶序列。

    Microarray for pathogen identification
    2.
    发明申请
    Microarray for pathogen identification 审中-公开
    微阵列用于病原体鉴定

    公开(公告)号：US20070092871A1

    公开(公告)日：2007-04-26

    申请号：US11584379

    申请日：2006-10-20

    申请人： Michael Lodes ,  Dominic Suciu

    发明人： Michael Lodes ,  Dominic Suciu

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/689 ,  C12Q1/6837 ,  C12Q1/705

    摘要： There is disclosed a microarray device for pathogen identification and for subtyping influenza A. Pools of primers are disclosed and used to amplify any subtype of influenza A. Pathogen identification includes influenza A, influenza B, parainfluenza virus, adenovirus, enterovirus, rhinovirus, human metapneumovirus, respiratory syncytal virus, herpes simplex viruses, SARS coronavirus, Epstein-Barr virus, human herpes virus, pan bacteria, Chlamydia, Mycoplasma, streptococcus, Bacillus anthracis, Streptococcuspyogenes, Mycoplasmapneumoniae, Chlamydiapneumoniae, Bacillus thuringiensis, Bacillus subtilis, Bacillus cereus, and B. anthracis. The probes are preferably selected from the first 500 nt of a gene from the 5′ end. The primers are preferably selected from bases in the 500 to 600 nt range from the 5′ end.

    摘要翻译： 披露了用于病原体鉴定和亚型流感病毒A的微阵列装置。公开了引物池，并用于扩增流感A的任何亚型。病原体鉴定包括甲型流感，乙型流感，副流感病毒，腺病毒，肠病毒，鼻病毒，人类偏肺病毒 ，呼吸道合胞病毒，单纯疱疹病毒，SARS冠状病毒，爱泼斯坦 - 巴尔病毒，人类疱疹病毒，泛菌，衣原体，支原体，链球菌，炭疽芽孢杆菌，链球菌瘟病毒，支原体肺炎支原体，衣原体肺炎链球菌，苏云金芽孢杆菌，枯草芽孢杆菌，蜡状芽孢杆菌和B 炭疽病 探针优选选自5'末端的基因的前500nt。 引物优选选自5'末端的500至600nt范围内的碱基。