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公开(公告)号：US20080032408A1
公开(公告)日：2008-02-07
申请号：US11562548
申请日：2006-11-22
IPC分类号： G01N33/00
CPC分类号： C12Q1/6837 , Y10T436/13 , C12Q2525/161
摘要： The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.
摘要翻译： 本发明提供了一组独特的核酸序列，其适用于需要核酸标签的各种应用。 因此，本发明的序列标签可用于例如在基因分型应用中和各种其它分析中标记生物和非生物材料。
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公开(公告)号：US07157564B1
公开(公告)日：2007-01-02
申请号：US09827383
申请日：2001-04-04
IPC分类号： C07H21/02
CPC分类号： C12Q1/6837 , Y10T436/13 , C12Q2525/161
摘要： The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.
摘要翻译： 本发明提供了一组独特的核酸序列，其适用于需要核酸标签的各种应用。 因此，本发明的序列标签可用于例如在基因分型应用中和各种其它分析中标记生物和非生物材料。
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公开(公告)号：US06806047B2
公开(公告)日：2004-10-19
申请号：US09776770
申请日：2001-02-06
申请人： Martin J. Goldberg , Govinda Rao S. Yelagalawadi , Eugene Yuji Tanimoto , Huu Minh Tran , Helin Dong , David Lockhart , Thomas B. Ryder , Janet A. Warrington , Jody Beecher
发明人： Martin J. Goldberg , Govinda Rao S. Yelagalawadi , Eugene Yuji Tanimoto , Huu Minh Tran , Helin Dong , David Lockhart , Thomas B. Ryder , Janet A. Warrington , Jody Beecher
IPC分类号： C12Q168
CPC分类号： C12Q1/682 , C12Q1/6804 , Y10S436/807 , C12Q2565/501 , C12Q2563/131 , C12Q2527/125 , C12Q2563/107 , C12Q2563/125 , C12Q2563/143 , C12Q2537/125
摘要： Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.
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公开(公告)号：US06203989B1
公开(公告)日：2001-03-20
申请号：US09276774
申请日：1999-03-25
申请人： Martin J. Goldberg , Govinda Rao S. Yelagalawadi , Eugene Yuji Tanimoto , Huu Minh Tran , Helin Dong , David Lockhart , Thomas B. Ryder , Janet A. Warrington , Jody Beecher
发明人： Martin J. Goldberg , Govinda Rao S. Yelagalawadi , Eugene Yuji Tanimoto , Huu Minh Tran , Helin Dong , David Lockhart , Thomas B. Ryder , Janet A. Warrington , Jody Beecher
IPC分类号： C12Q168
CPC分类号： C12Q1/682 , C12Q1/6804 , Y10S436/807 , C12Q2565/501 , C12Q2563/131 , C12Q2527/125 , C12Q2563/107 , C12Q2563/125 , C12Q2563/143 , C12Q2537/125
摘要： Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.
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公开(公告)号：US06582938B1
公开(公告)日：2003-06-24
申请号：US09854317
申请日：2001-05-11
申请人： Xing Su , Helin Dong , Thomas B. Ryder
发明人： Xing Su , Helin Dong , Thomas B. Ryder
IPC分类号： C12P1934
CPC分类号： C12Q1/6865
摘要： The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction.
摘要翻译： 本发明涉及核酸，优选mRNA的扩增。 将引物和启动子加入要扩增的靶序列中，然后在体外转录反应中扩增靶。
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公开(公告)号：US5856088A
公开(公告)日：1999-01-05
申请号：US462646
申请日：1995-06-05
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type from other viruses.
摘要翻译： 扩增寡核苷酸和杂交测定探针，将人类免疫缺陷病毒类型与其他病毒区分开来。
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公开(公告)号：US06252059B1
公开(公告)日：2001-06-26
申请号：US09013406
申请日：1998-01-26
IPC分类号： C07H2104
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.
摘要翻译： 扩增寡核苷酸和杂交测定探针，将1型人类免疫缺陷病毒与其他病毒区分开来。
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公开(公告)号：US5786183A
公开(公告)日：1998-07-28
申请号：US421471
申请日：1995-04-14
CPC分类号： C12Q1/6844 , C12Q1/686 , C12Q1/6865
摘要： A method for amplification of a nucleic acid strand in a test sample. The method includes contacting the nucleic acid strand from the test sample simultaneously with at least three oligonucleotide primers. At least one primer is a promoter-primer, and at least one other primer is complementary to the nucleic acid strand, and one other primer is complementary to a strand complementary to the nucleic acid strand. The method further includes contacting the nucleic acid strand and primers with one or more proteins having RNA-directed and/or DNA-directed DNA polymerase activities, an RNA polymerase activity, and an RNAse H activity under primer-extension conditions to allow amplification of a target region in the nucleic acid strand at essentially constant temperature.
摘要翻译： 一种用于在测试样品中扩增核酸链的方法。 该方法包括使来自测试样品的核酸链与至少三种寡核苷酸引物同时接触。 至少一个引物是启动子 - 引物，并且至少一个其它引物与核酸链互补，并且另外一个引物与与核酸链互补的链互补。 该方法还包括在引物延伸条件下使核酸链和引物与一种或多种具有RNA指导和/或DNA指导的DNA聚合酶活性，RNA聚合酶活性和RNA酶H活性的蛋白质接触，以允许扩增 核酸链中的靶区域基本恒定。
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公开(公告)号：US5712385A
公开(公告)日：1998-01-27
申请号：US479852
申请日：1995-06-07
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.
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公开(公告)号：US06649749B2
公开(公告)日：2003-11-18
申请号：US09766095
申请日：2001-01-20
IPC分类号： C07H2104
CPC分类号： C12Q1/703 , Y10S435/81
摘要： Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.
摘要翻译： 扩增寡核苷酸和杂交测定探针，将1型人类免疫缺陷病毒与其他病毒区分开来。
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