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    NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF
    4.
    发明申请
    NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF 审中-公开
    正规化的核酸图书馆及其生产方法

    公开(公告)号：US20120071354A1

    公开(公告)日：2012-03-22

    申请号：US13214976

    申请日：2011-08-22

    申请人： Wu-Bo Li ,  Paul E. Nisson ,  Joel Jessee

    发明人： Wu-Bo Li ,  Paul E. Nisson ,  Joel Jessee

    IPC分类号： C40B40/02 ,  C40B50/06 ,  C40B40/06 ,  C40B50/00

    CPC分类号： G10L21/01

    摘要： The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.

    摘要翻译： 本发明一般涉及用于产生归一化核酸文库的方法，其中文库的每个成员可以以大致等同的概率分离。 特别地，本发明的方法包括核酸文库与与文库的一个或多个核酸分子互补的半抗原化（例如，生物素化，吸入或链霉亲和的）核酸分子的消减杂交，使得 文库中各个核酸分子的丰度降低。 本发明还涉及其中污染性核酸分子被还原或消除的标准化核酸文库（特别是cDNA文库）以及通过这些方法产生的标准化的核酸文库。

    NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF
    6.
    发明申请
    NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF 审中-公开

    公开(公告)号：US20090264304A1

    公开(公告)日：2009-10-22

    申请号：US11851353

    申请日：2007-09-06

    申请人： Wu-Bo Li ,  Paul E. Nisson ,  Joel Jessee

    发明人： Wu-Bo Li ,  Paul E. Nisson ,  Joel Jessee

    IPC分类号： C40B30/04

    CPC分类号： C12N15/1093 ,  C07H21/00 ,  C12N15/1096 ,  C12Q2563/131 ,  C12Q2600/158 ,  C40B30/06 ,  C40B30/08

    摘要： The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.

    Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules
    7.
    发明授权
    Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules 有权
    通过增强编码核酸分子的合成产生多肽的方法

    公开(公告)号：US07344863B2

    公开(公告)日：2008-03-18

    申请号：US10899139

    申请日：2004-07-27

    申请人： Wu-Bo Li ,  Joel A. Jessee ,  David Schuster ,  Jiulin Xia ,  Gulilat Gebeyehu

    发明人： Wu-Bo Li ,  Joel A. Jessee ,  David Schuster ,  Jiulin Xia ,  Gulilat Gebeyehu

    IPC分类号： C12P21/00 ,  C12P19/34

    CPC分类号： C12P19/34 ,  C07H21/00 ,  C12Q1/6848 ,  C12Q2527/125

    摘要： The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

    摘要翻译： 本发明涉及用于增强核酸分子，特别是富含GC的核酸分子的合成的组合物和方法。 具体地说，本发明提供包含一种或多种具有选自式I和式II（或其盐或衍生物），优选4-甲基吗啉N-氧化物或甜菜碱（羧甲基三甲基铵）的式的含氮有机化合物的组合物， 并且还包含一种或多种选自脯氨酸和N-烷基咪唑化合物，更优选脯氨酸，1-甲基咪唑或4-甲基咪唑的化合物。 本发明还涉及用于核酸分子增强，高保真合成的方法，包括经由扩增（特别是PCR），逆转录和测序方法。 本发明还涉及通过这些方法合成的核酸分子，其片段或衍生物以及包含该核酸分子，片段或衍生物的载体和宿主细胞。 本发明还涉及用于合成，扩增，逆转录或测序包含一种或多种本发明组合物的核酸分子的试剂盒。

    Compositions and methods for enhanced synthesis of nucleic acid molecules
    8.
    发明授权
    Compositions and methods for enhanced synthesis of nucleic acid molecules 有权
    用于增强核酸分子合成的组合物和方法

    公开(公告)号：US06787305B1

    公开(公告)日：2004-09-07

    申请号：US09266935

    申请日：1999-03-12

    申请人： Wu-Bo Li ,  Joel A. Jessee ,  David Schuster ,  Jiulin Xia ,  Gulilat Gebeyehu

    发明人： Wu-Bo Li ,  Joel A. Jessee ,  David Schuster ,  Jiulin Xia ,  Gulilat Gebeyehu

    IPC分类号： C12Q168

    CPC分类号： C12P19/34 ,  C07H21/00 ,  C12Q1/6848 ,  C12Q2527/125

    摘要： The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methyliimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

    摘要翻译： 本发明涉及用于增强核酸分子，特别是富含GC的核酸分子的合成的组合物和方法。 具体地说，本发明提供包含一种或多种具有选自式I和式II（或其盐或衍生物），优选4-甲基吗啉N-氧化物或甜菜碱（羧甲基三甲基铵）的式的含氮有机化合物的组合物， 并且还包含一种或多种选自脯氨酸和N-烷基咪唑化合物的化合物，更优选脯氨酸，1-甲基咪唑或4-甲基咪唑。 本发明还涉及用于核酸分子增强，高保真合成的方法，包括经由扩增（特别是PCR），逆转录和测序方法。 本发明还涉及通过这些方法合成的核酸分子，其片段或衍生物以及包含该核酸分子，片段或衍生物的载体和宿主细胞。 本发明还涉及用于合成，扩增，逆转录或测序包含一种或多种本发明组合物的核酸分子的试剂盒。

    Normalized nucleic acid libraries and methods of production thereof
    9.
    发明授权
    Normalized nucleic acid libraries and methods of production thereof 有权

    公开(公告)号：US06399334B1

    公开(公告)日：2002-06-04

    申请号：US09159496

    申请日：1998-09-23

    申请人： Wu-Bo Li ,  Paul E. Nisson ,  Joel Jessee

    发明人： Wu-Bo Li ,  Paul E. Nisson ,  Joel Jessee

    IPC分类号： C12P1934

    CPC分类号： C12N15/1093 ,  C07H21/00 ,  C12N15/1096 ,  C12Q2563/131 ,  C12Q2600/158 ,  C40B30/06 ,  C40B30/08

    摘要： The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.

    NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF
    10.
    发明申请
    NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF 审中-公开
    正规化的核酸图书馆及其生产方法

    公开(公告)号：US20100279898A1

    公开(公告)日：2010-11-04

    申请号：US12762866

    申请日：2010-04-19

    申请人： Wu-Bo Li ,  Paul E. Nisson ,  Joel JESSEE

    发明人： Wu-Bo Li ,  Paul E. Nisson ,  Joel JESSEE

    IPC分类号： C40B50/00

    CPC分类号： G10L21/01

    摘要： The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.

    摘要翻译： 本发明一般涉及用于产生归一化核酸文库的方法，其中文库的每个成员可以以大致等同的概率分离。 特别地，本发明的方法包括核酸文库与与文库的一个或多个核酸分子互补的半抗原化（例如，生物素化，吸入或链霉亲和的）核酸分子的消减杂交，使得 文库中各个核酸分子的丰度降低。 本发明还涉及其中污染性核酸分子被还原或消除的标准化核酸文库（特别是cDNA文库）以及通过这些方法产生的标准化的核酸文库。