公开(公告)号：US09073053B2

公开(公告)日：2015-07-07

申请号：US11233629

申请日：2005-09-22

申请人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching

发明人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching

IPC分类号： C12N1/06 ,  B01L3/00 ,  C12M1/00

CPC分类号： B01L3/502 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  C12M47/06

摘要： An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

摘要翻译： 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：US08268603B2

公开(公告)日：2012-09-18

申请号：US11234656

申请日：2005-09-22

申请人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Jesus Ching

发明人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Jesus Ching

IPC分类号： C12N13/00 ,  C12M1/33 ,  C12M3/08

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06

摘要： An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

摘要翻译： 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：US20120171758A1

公开(公告)日：2012-07-05

申请号：US11977615

申请日：2007-10-24

申请人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

发明人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

IPC分类号： C12M1/34 ,  C12M1/00

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

摘要： The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.

摘要翻译： 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体，裂解细胞或病毒以从其中分离分析物（例如核酸），以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断，生命科学研究，环境或法医应用，用于确定样品中一种或多种分析物的存在或不存在。

公开(公告)号：US20120009667A1

公开(公告)日：2012-01-12

申请号：US13081060

申请日：2011-04-06

申请人： KURT E. PETERSON ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

发明人： KURT E. PETERSON ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

IPC分类号： C12M1/34 ,  C12M1/12 ,  C12M1/00

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

摘要： The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.

摘要翻译： 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体，裂解细胞或病毒以从其中分离分析物（例如核酸），以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断，生命科学研究，环境或法医应用，用于确定样品中一种或多种分析物的存在或不存在。

公开(公告)号：US08580559B2

公开(公告)日：2013-11-12

申请号：US11977615

申请日：2007-10-24

申请人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

发明人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

IPC分类号： C12M1/34

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

摘要： The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.

摘要翻译： 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体，裂解细胞或病毒以从其中分离分析物（例如核酸），以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断，生命科学研究，环境或法医应用，用于确定样品中一种或多种分析物的存在或不存在。

公开(公告)号：US06881541B2

公开(公告)日：2005-04-19

申请号：US09800590

申请日：2001-03-06

申请人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

发明人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

IPC分类号： C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12Q1/68

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

摘要： A method for extracting nucleic acid from a fluid sample comprises the steps of introducing the sample into a cartridge having a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for separating cells or viruses from the sample. The sample is forced to flow through the lysing chamber to capture the cells or viruses with the filter, while used sample fluid flows to waste. The captured cells or viruses are disrupted to release their nucleic acid, the nucleic acid is eluted from the lysing chamber, and optionally the nucleic acid is amplified and detected in a reaction chamber of the cartridge.

摘要翻译： 从流体样品中提取核酸的方法包括以下步骤：将样品引入具有样品流动路径中的样品流动路径和裂解室的盒中。 裂解室含有至少一个用于从样品中分离细胞或病毒的过滤器。 样品被迫流过裂解室以用过滤器捕获细胞或病毒，而使用的样品流体流入废物。 捕获的细胞或病毒被破坏以释放其核酸，核酸从裂解室洗脱，并且任选地在核心的反应室中扩增和检测核酸。

公开(公告)号：US09789481B2

公开(公告)日：2017-10-17

申请号：US11977697

申请日：2007-10-24

申请人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

发明人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12N1/06 ,  C12N13/00

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

摘要： The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.

公开(公告)号：US06391541B1

公开(公告)日：2002-05-21

申请号：US09583807

申请日：2000-05-30

申请人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

发明人： Kurt E. Petersen ,  Michael T. Taylor ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching ,  Douglas B. Dority ,  Phillip Belgrader ,  M. Allen Northrup

IPC分类号： G01N3353

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

摘要： A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

摘要翻译： 用于从流体样品分离所需分析物的滤筒具有样品流动路径中的样品流动路径和裂解室。 裂解室包含至少一个用于在样品流过裂解室时从样品捕获细胞或病毒的过滤器。 珠也设置在裂解室中，用于破裂细胞或病毒以从其中释放分析物。 分析物流动路径从裂解室延伸并从样品流动路径发散。 分析物流动路径优选地导致用于化学反应和光学检测分析物的反应室。 该盒还包括至少一个流量控制器（例如阀），用于在样品流过裂解室之后将样品引入废物室，并将从样品分离的分析物引导到分析物流动路径中。

公开(公告)号：US06987018B2

公开(公告)日：2006-01-17

申请号：US10208976

申请日：2002-07-30

申请人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Douglas B. Dority

发明人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Douglas B. Dority

IPC分类号： C12M1/34

CPC分类号： B01L3/502 ,  B01L3/50825 ,  B01L7/52 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/14 ,  B01L2400/0439 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06

摘要： A container for holding cells or viruses for disruption comprises a chamber defined by two spaced apart, opposing major walls and side walls connecting the major walls to each other. At least one of the major walls has an external surface to which the transducer may be coupled and is sufficiently flexible to flex in response to vibratory motion of the transducer. The container also has at least one port for introducing the cells or viruses into the chamber. In some embodiments, the chamber contains beads for aiding the disruption of the cells or viruses.

公开(公告)号：US06431476B1

公开(公告)日：2002-08-13

申请号：US09469724

申请日：1999-12-21

申请人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Douglas B. Dority

发明人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Douglas B. Dority

IPC分类号： B02C1900

CPC分类号： B01L3/502 ,  B01L3/50825 ,  B01L7/52 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/14 ,  B01L2400/0439 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06

摘要： The present invention provides an apparatus and method for disrupting cells or viruses to release the nucleic acid therefrom. The apparatus includes a container having a chamber for holding the cells or viruses. The apparatus also includes an ultrasonic transducer for contacting a wall of the chamber and for transmitting ultrasonic energy into the chamber through the wall. A support structure holds the container and the transducer against each other such that the transducer contacts the wall of the chamber. The support structure includes an elastic body, such as a spring, for applying to the container or to the transducer a substantially constant force to press together the transducer and the wall. The chamber also preferably contains beads for enhancing the disruption of the cells or viruses. The apparatus performs rapid and consistent lysis of cells or viruses, often in as little time as 5 to 10 seconds.

摘要翻译： 本发明提供了用于破坏细胞或病毒从其释放核酸的装置和方法。 该装置包括具有用于保持细胞或病毒的室的容器。 该装置还包括用于接触室的壁并用于通过壁将超声波能量传递到室中的超声换能器。 支撑结构将容器和换能器彼此抵靠，使得换能器接触室的壁。 支撑结构包括诸如弹簧的弹性体，用于向容器或换能器施加基本恒定的力以将换能器和壁压在一起。 该室还优选含有用于增强细胞或病毒破坏的珠粒。 该装置通常在5至10秒的短时间内快速且一致地裂解细胞或病毒。