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公开(公告)号：US20120295269A1
公开(公告)日：2012-11-22
申请号：US13552950
申请日：2012-07-19
Applicant: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
Inventor： Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
CPC classification number: C12N15/1017 , B01D15/00 , B01L7/52 , B01L2400/0622 , B01L2400/0644 , C12M47/06 , G01N1/34 , Y10T436/255
Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
Abstract translation: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。
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公开(公告)号：US20110165562A1
公开(公告)日：2011-07-07
申请号：US13042203
申请日：2011-03-07
Applicant: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
Inventor： Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
CPC classification number: C12N15/1017 , B01D15/00 , B01L7/52 , B01L2400/0622 , B01L2400/0644 , C12M47/06 , G01N1/34 , Y10T436/255
Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
Abstract translation: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。
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公开(公告)号：US20100068706A1
公开(公告)日：2010-03-18
申请号：US12370527
申请日：2009-02-12
Applicant: Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
Inventor： Farzad Pourahmadi , William A. McMillan , Jesus Ching , Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , M. Allen Northrup , Kurt E. Petersen
CPC classification number: C12N15/1017 , B01D15/00 , B01L7/52 , B01L2400/0622 , B01L2400/0644 , C12M47/06 , G01N1/34 , Y10T436/255
Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
Abstract translation: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。
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4.发明授权公开(公告)号：US07297313B1
公开(公告)日：2007-11-20
申请号：US08482933
申请日：1995-06-07
Applicant: M. Allen Northrup , Richard M. White
Inventor： M. Allen Northrup , Richard M. White
CPC classification number: B01L7/52 , B01L3/502707 , B01L7/525 , B01L9/527 , B01L2300/1827 , B01L2400/0406 , B01L2400/0436 , G01N9/24 , G01N11/16
Abstract: An integrated microfabricated instrument for manipulation, reaction and detection of microliter to picoliter samples. The instrument is suited for biochemical reactions, particularly DNA-based reactions such as the polymerase chain reaction, that require thermal cycling since the inherently small size of the instrument facilitates rapid cycle times. The integrated nature of the instrument provides accurate, contamination-free processing. The instrument may include reagent reservoirs, agitators and mixers, heaters, pumps, and optical or electromechanical sensors. Ultrasonic Lamb-wave devices may be used as sensors, pumps and agitators.
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公开(公告)号：US06979424B2
公开(公告)日：2005-12-27
申请号：US09929270
申请日：2001-08-13
Applicant: M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs
Inventor： M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs
CPC classification number: G01N27/44743 , B01J19/0093 , B01L3/502738 , B01L3/502753 , B01L2300/1883 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0633 , B01L2400/0655 , G01N30/6095
Abstract: An analysis device comprises a body having a reaction chamber for chemically reacting a sample, a separation region for separating components of the sample, and a transition region connecting the reaction chamber to the separation region. The transition region includes at least one valve for controlling the flow of fluid between the reaction chamber and the separation region. Further, the transition region thermally isolates the reaction chamber from the separation region. In a preferred embodiment, the reaction chamber is an amplification chamber for amplifying nucleic acid in the sample, and the separation region comprises an electrophoresis channel containing a suitable matrix material, such as electrophoresis gel or buffer, for separating nucleic acid fragments. Electrodes are embedded in the body for separation of sample components. The body may also be surrounded by external, functional components such as an optical detector for detecting separated components of the sample.
Abstract translation: 分析装置包括具有用于使样品化学反应的反应室的主体，用于分离样品的组分的分离区域和将反应室连接到分离区域的过渡区域。 过渡区域包括用于控制反应室和分离区域之间的流体流动的至少一个阀。 此外，过渡区域将反应室与分离区域热隔离。 在一个优选的实施方案中，反应室是用于扩增样品中的核酸的扩增室，分离区域包含一个电泳通道，其中含有合适的基质材料，如电泳凝胶或缓冲液，用于分离核酸片段。 电极被嵌入在体内用于分离样品组分。 身体也可以被外部功能部件包围，例如用于检测样品的分离成分的光学检测器。
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Patent Agency Ranking
公开(公告)号：US06940598B2
公开(公告)日：2005-09-06
申请号：US10119228
申请日：2002-04-08
Applicant: Lee A. Christel , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs , Steven J. Young , Ronald Chang , Douglas B. Dority , Raymond T. Hebert , Gregory J. Kintz
Inventor： Lee A. Christel , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs , Steven J. Young , Ronald Chang , Douglas B. Dority , Raymond T. Hebert , Gregory J. Kintz
IPC: B01J19/00 , B01L3/00 , B01L3/14 , B01L7/00 , F28F3/12 , F28F21/04 , G01N21/03 , G01N35/00 , G01N21/64
CPC classification number: B01L3/508 , B01J19/0093 , B01L3/5082 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
Abstract: An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel [2] having a chamber [10] for holding the mixture. The apparatus also includes a heat-exchanging module [37] having a pair of opposing thermal plates [34A, 34B] for receiving the vessel [2] between them and for heating/and or cooling the mixture contained in the vessel. The module [37] also includes optical excitation and detection assemblies [46,48] positioned to optically interrogate the mixture. The excitation assembly [46] includes multiple light sources [100] and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly [48] includes multiple detectors [102] and a second set of filters for detecting light emitted from the chamber [10] in multiple emission wavelength ranges. The optics assemblies [46,48] thus provide a multi-channel system for detecting a plurality of different target analytes in the mixture.
Abstract translation: 用于热控制和光学询问反应混合物的装置包括具有用于保持混合物的室[10]的容器[2]。 该装置还包括具有一对相对的热板[34A，34B]的热交换模块[37]，用于在它们之间接收容器[2]并用于加热和/或冷却容器中所含的混合物。 模块[37]还包括定位成光学询问混合物的光学激发和检测组件[46,48]。 激发组件[46]包括多个光源[100]和一组用于在多个激发波长范围内用激发光束顺序地照射混合物中的标记分析物的滤光器。 检测组件[48]包括多个检测器[102]和第二组滤波器，用于在多个发射波长范围内检测从腔室[10]发射的光。 因此，光学组件[46,48]提供用于检测混合物中的多个不同目标分析物的多通道系统。
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公开(公告)号：US06524532B1
公开(公告)日：2003-02-25
申请号：US08763465
申请日：1996-12-11
Applicant: M. Allen Northrup
Inventor： M. Allen Northrup
IPC: B01L300
CPC classification number: B01L7/52 , B01J19/0046 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00824 , B01J2219/00828 , B01J2219/00853 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/00912 , B01J2219/00936 , B01J2219/00945 , B01J2219/00952 , B01L3/5027 , B01L3/502707 , B01L9/527 , B01L2200/027 , B01L2200/028 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0654 , B01L2300/0867 , B01L2300/087 , B01L2300/1822 , B01L2300/1827 , B01L2400/0415 , B01L2400/0421 , B01L2400/0439 , B01L2400/0487 , B81C3/00 , G01N21/0332 , G01N21/05 , G01N21/69 , G01N21/76 , G01N2021/0346
Abstract: A silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and non-silicon based materials to provide the thermal properties desired. For example, the chamber may combine a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.
Abstract translation: 一种硅基套管型化学反应室，其结合加热器，例如用于加热的掺杂多晶硅和用于对流冷却的体硅。 反应室结合硅和非硅基材料的临界比以提供所需的热性质。 例如，室可将硅和氮化硅的临界比与待加热材料（例如液体）的体积组合，以便提供均匀的加热，但低功率要求。 反应室还将允许将二次管（例如塑料）引入到包含反应混合物的反应套筒中，从而减轻任何潜在的材料不相容性问题。 反应室可用于任何用于合成或加工有机，无机或生物化学反应的化学反应体系，例如聚合酶链式反应（PCR）和/或其它DNA反应，例如连接酶链式反应，其为实施例 的合成，热循环反应。 反应室也可用于合成仪器，特别是用于DNA扩增和合成的仪器。
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公开(公告)号：US5819749A
公开(公告)日：1998-10-13
申请号：US898032
申请日：1997-07-22
Applicant: Abraham P. Lee , Peter A. Krulevitch , M. Allen Northrup , Jimmy C. Trevino
Inventor： Abraham P. Lee , Peter A. Krulevitch , M. Allen Northrup , Jimmy C. Trevino
CPC classification number: A61B17/12022 , G01L1/18 , A61B17/29 , A61B2017/00292 , A61B2017/00318 , A61B2017/00345 , A61B2017/1205 , A61B2017/2926
Abstract: Micromachined thin film cantilever actuators having means for individually controlling the deflection of the cantilevers, valve members, and rudders for steering same through blood vessels, or positioning same within a blood vessel, for example. Such cantilever actuators include tactile sensor arrays mounted on a catheter or guide wire tip for navigation and tissues identification, shape-memory alloy film based catheter/guide wire steering mechanisms, and rudder-based steering devices that allow the selective actuation of rudders that use the flowing blood itself to help direct the catheter direction through the blood vessel. While particularly adapted for medical applications, these cantilever actuators can be used for steering through piping and tubing systems.
Abstract translation: 微加工薄膜悬臂致动器具有用于单独控制悬臂，阀构件和用于通过血管转向其的方向舵的偏转的装置，或者例如将其定位在血管内。 这种悬臂致动器包括安装在用于导航和组织识别的导管或导丝尖端的触觉传感器阵列，基于形状记忆合金膜的导管/导丝线转向机构和基于方向舵的转向装置，其允许选择性地致动使用 流血本身帮助导管导管通过血管。 虽然这些悬臂式致动器特别适用于医疗应用，但可用于通过管道和管道系统进行转向。
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公开(公告)号：US09789481B2
公开(公告)日：2017-10-17
申请号：US11977697
申请日：2007-10-24
Applicant: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
Inventor： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
CPC classification number: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
Abstract: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
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公开(公告)号：US09156032B2
公开(公告)日：2015-10-13
申请号：US13081060
申请日：2011-04-06
Applicant: Kurt E. Peterson , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas P. Dority , Philip Belgrader , M. Allen Northrup
Inventor： Kurt E. Peterson , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas P. Dority , Philip Belgrader , M. Allen Northrup
CPC classification number: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
Abstract: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
Abstract translation: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体，裂解细胞或病毒以从其中分离分析物（例如核酸），以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断，生命科学研究，环境或法医应用，用于确定样品中一种或多种分析物的存在或不存在。
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