利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.036 秒)

    METHOD FOR IDENTIFYING AND SELECTING DRUG CANDIDATES FOR COMBINATORIAL DRUG PRODUCTS
    4.
    发明申请
    METHOD FOR IDENTIFYING AND SELECTING DRUG CANDIDATES FOR COMBINATORIAL DRUG PRODUCTS 审中-公开
    识别和选择用于组合药物制品的药物候选药物的方法

    公开(公告)号：US20110224094A1

    公开(公告)日：2011-09-15

    申请号：US13122713

    申请日：2009-10-06

    申请人： Mikkel Wandahl Pedersen ,  Per-Johan Meijer ,  Allan Jensen

    发明人： Mikkel Wandahl Pedersen ,  Per-Johan Meijer ,  Allan Jensen

    IPC分类号： C40B30/04 ,  C40B30/06 ,  C40B30/00 ,  C40B30/08

    CPC分类号： G01N33/6854 ,  A61K2039/507 ,  C07K16/00 ,  C07K16/2863 ,  G01N33/6845

    摘要： A method for identifying and selecting chemical entities that contributes to a functional effect in the development of new combinatorial drugs. The combinations of two or more chemical compounds show a synergistic effect. The compounds can be e.g. antibodies, antibiotics, anti-cancer agents, anti-AIDS agents, anti-growth factors, antiviral agents, soluble receptors, cytokines, RNAi's, vaccines and mixtures thereof. The method comprises a) providing n samples each comprising a chemical entity, b) mixing 2 or more of the n samples in all possible combinations, c) subjecting this mixture to a functional assay in order to identify entities contributing to the functional effect. The steps a-c are repeated on the chemical entities from step c which contribute to the functional effect.

    摘要翻译： 用于鉴定和选择有助于开发新组合药物的功能效应的化学物质的方法。 两种或更多种化合物的组合显示出协同效应。 化合物可以是例如 抗体，抗生素，抗癌剂，抗艾滋病剂，抗生长因子，抗病毒剂，可溶性受体，细胞因子，RNAi，疫苗及其混合物。 该方法包括a）提供每个包含化学实体的n个样品，b）以所有可能的组合混合2个或更多个n个样品，c）使该混合物进行功能测定，以鉴定有助于功能效应的实体。 对来自步骤c的有助于功能效应的化学实体重复步骤a-c。

    Method for cloning cognate antibodies
    7.
    发明申请
    Method for cloning cognate antibodies 有权
    克隆同源抗体​​的方法

    公开(公告)号：US20080227660A1

    公开(公告)日：2008-09-18

    申请号：US12074066

    申请日：2008-02-29

    申请人： Jesper Kastrup ,  Lars S. Nielsen ,  Per-Johan Meijer

    发明人： Jesper Kastrup ,  Lars S. Nielsen ,  Per-Johan Meijer

    IPC分类号： C40B40/08 ,  C40B50/06

    CPC分类号： C12N15/1093 ,  C07K16/005 ,  C07K16/1282 ,  C07K16/2863 ,  C07K2317/24 ,  C07K2317/73

    摘要： The invention relates to a procedure for linking cognate pairs of VH and VL encoding sequences from a population of cells enriched in particular surface antigen markers. The linking procedure involves a multiplex molecular amplification procedure capable of linking nucleotide sequences of interest in connection with the amplification, in particular polymerase chain reaction (multiplex PCR). The method is particularly advantageous for the generation of cognate pair libraries as well as combinatorial libraries of variable region encoding sequences from immunoglobulins. The invention also relates to methods for generation of chimeric human/non-human antibodies and expression libraries generated by such methods.

    摘要翻译： 本发明涉及从富集特定表面抗原标记的细胞群中连接编码序列的同源对的步骤。 连接方法涉及能够连接与扩增有关的核苷酸序列的多重分子扩增方法，特别是聚合酶链式反应（多重PCR）。 该方法特别有利于产生同源对文库以及来自免疫球蛋白的可变区编码序列的组合文库。 本发明还涉及用于产生嵌合人/非人抗体的方法和通过这些方法产生的表达文库。

    Method for Cloning Cognate Antibodies
    8.
    发明申请
    Method for Cloning Cognate Antibodies 审中-公开
    克隆认知抗体的方法

    公开(公告)号：US20130023447A1

    公开(公告)日：2013-01-24

    申请号：US13617778

    申请日：2012-09-14

    申请人： Jesper KASTRUP ,  Lars S. Nielsen ,  Per-Johan Meijer

    发明人： Jesper KASTRUP ,  Lars S. Nielsen ,  Per-Johan Meijer

    IPC分类号： C12P19/34 ,  C40B40/08

    CPC分类号： C12N15/1093 ,  C07K16/005 ,  C07K16/1282 ,  C07K16/2863 ,  C07K2317/24 ,  C07K2317/73

    摘要： The invention relates to a procedure for linking cognate pairs of VH and VL encoding sequences from a population of cells enriched in particular surface antigen markers. The linking procedure involves a multiplex molecular amplification procedure capable of linking nucleotide sequences of interest in connection with the amplification, in particular polymerase chain reaction (multiplex PCR). The method is particularly advantageous for the generation of cognate pair libraries as well as combinatorial libraries of variable region encoding sequences from immunoglobulins. The invention also relates to methods for generation of chimeric human/non-human antibodies and expression libraries generated by such methods.

    摘要翻译： 本发明涉及从富集特定表面抗原标记的细胞群中连接VH和VL编码序列的同源对的方法。 连接方法涉及能够连接与扩增有关的核苷酸序列的多重分子扩增方法，特别是聚合酶链式反应（多重PCR）。 该方法特别有利于产生同源对文库以及来自免疫球蛋白的可变区编码序列的组合文库。 本发明还涉及用于产生嵌合人/非人抗体的方法和通过这些方法产生的表达文库。

    Screening for expressible transfectants in a eukaryotic system
    9.
    发明申请
    Screening for expressible transfectants in a eukaryotic system 审中-公开
    筛选真核系统中可表达的转染子

    公开(公告)号：US20080299581A1

    公开(公告)日：2008-12-04

    申请号：US12153804

    申请日：2008-05-23

    申请人： Lars Soegaard Nielsen ,  Per-Johan Meijer

    发明人： Lars Soegaard Nielsen ,  Per-Johan Meijer

    IPC分类号： C12Q1/68 ,  C12P21/04

    CPC分类号： C12N15/1093

    摘要： The present invention relates to the field of molecular cloning and to the field of expression cloning in higher, eukaryotic cells. In particular, the present invention relates to a method for fast and reliable identification of vectors and vector DNA which will be capable of providing a desired expression product if a eukaryotic host cell is transfected with the vector DNA.

    摘要翻译： 本发明涉及分子克隆领域以及较高真核细胞中表达克隆领域。 特别地，本发明涉及一种用于快速可靠地鉴定载体和载体DNA的方法，如果用载体DNA转染真核宿主细胞，其能够提供所需的表达产物。

    Germination-specific plant promoters
    10.
    发明授权
    Germination-specific plant promoters 失效
    萌发特异性植物启动子

    公开(公告)号：US06359196B1

    公开(公告)日：2002-03-19

    申请号：US09404390

    申请日：1999-09-23

    申请人： Finn Lok ,  Ole Olsen ,  Per-Johan Meijer ,  Verena Cameron-Mills

    发明人： Finn Lok ,  Ole Olsen ,  Per-Johan Meijer ,  Verena Cameron-Mills

    IPC分类号： A01H100

    CPC分类号： C12Y302/01031 ,  C07K14/8139 ,  C12N9/2408 ,  C12N15/8234 ,  C12N15/8238 ,  C12N15/8242 ,  C12Y302/0102 ,  C12Y302/01073

    摘要： Tissue-specific regulatory sequences, including promoters from barley (Hordeum vulgare) genes encoding &agr;-glucosidase and cystatin-1 (cysteine protease inhibitor) have been identified and isolated. The gene promoters have particular utility in directing high level, germination preferred expression of heterologous proteins that impart increased agronomic and malting characteristics to a given plant. In addition, the invention relates to methods of producing transgenic plants by using chimeric genes, cassette vectors, kits, cells and methods comprising the promoter and signal sequence coding region of the gene for barley &agr;-glucosidase and the gene for barley cystatin-1.

    摘要翻译： 已经鉴定并分离了组织特异性调节序列，包括来自编码α-葡糖苷酶和半胱氨酸蛋白酶抑制剂（半胱氨酸蛋白酶抑制剂）的大麦（Hordeum vulgare）基因的启动子。 基因启动子在指导异源蛋白质的高水平发芽优选表达方面具有特别的用途，赋予给定植物更多的农艺和麦芽特征。 此外，本发明涉及通过使用嵌合基因，盒载体，试剂盒，细胞和包含大麦α-葡糖苷酶基因的启动子和信号序列编码区和大麦半胱氨酸蛋白酶抑制蛋白-1基因的方法来生产转基因植物的方法。