摘要:
The method for the prevention, reduction and/or removal of asphaltene-paraffin-vax precipitates on surfaces in contact with crude oil within oil-wells from bottom hole to well head, in flow lines and pipelines by the use of biocolloid suspensions comprises the steps of: adding together (a) microorganism(s) capable of reacting with, solubilizing of and/or resistant to the components of crude oil, (b) organic or inorganic additives, or (c) a mixture thereof into the pipes; and allowing the so formed suspension containing the microorganisms to act for a desired time. The suspension is used for the decomposition, washing-off and removal of solid hydrocarbons and mixtures of long chain hydrocarbons, as well as for the prevention of formation thereof.
摘要:
The method for preventing the formation of oil-win-water and/or water-in-oil emulsions and/or for breaking up emulsions already formed, comprises the steps of: (a) adding tensides, materials for increasing viscosity, industrial surfactants, and microorganisms capable of breaking down crude oil components or derivatives and producing at least one type of tenside, to the emulsion already formed or into the device containing the crude oil in which the formation of the emulsion to be prevented, optionally together with additives required for the reproduction of said microorganisms; (b) providing an appropriate temperature for the microorganisms after the addition of the materials listed above; (c) allowing the microorganisms to reproduce and act for a predetermined period of time; (d) checking the results of the treatment; and (e) optionally repeating steps (a) to (d) at least once more, preferably at least three more times.
摘要:
The invention relates to methods for land improvement (soil reclamation), preferably for soil remediation comprising the steps of (i) placing a material useful for improving soil, and an explosive in the soil, and (ii) mixing the materials useful for reclaiming the soil and the polluted soil by explosion. The invention also relates to methods methods for providing microorganisms useful for decomposing hydrophobic pollutants, mineral oil components and derivatives by selection and isolation from soil, the microorganisms, their uses and kits for land improvement and soil remediation. The invention is useful in particular for remediation of soil polluted with oil components and derivatives.
摘要:
A structural gene coding for authentic human serum albumin, --optionally supplemented by an upstream triplet coding for methionine and optionally extended by a synthetic prepro.sup.x -leader-coding sequence--, wherein the codons of the nucleotide sequence have been selected with regard to a non-human host, e.g. yeast, chosen for expression of authentic human serum albumin, is disclosed.Additionally there is disclosed a method of producing said gene.There are also disclosed a recombinant DNA molecule comprising said strucural gene inserted into a vector, and a host transformed with said recombinant DNA molecule.Furthermore there are disclosed a method of producing authentic human serum albumin, an authentic human serum albumin resulting from said method, and a pharmaceutical composition comprising said resulting human serum albumin.
摘要:
The invention relates to a process for the preparation of oligo- and polydeoxyribonucleotides by synthesizing the complementary strand of a single-stranded DNA piece enzymatically, in the presence of deoxyribonucleoside 5'-triphosphates in a cloning vector.
摘要:
The invention relates to a process for the immobilization of compounds comprising nucleophilic groups.According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273.degree.-343.degree. K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260.degree.-313.degree. K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing.