Method for the treatment and prevention of asphaltene-paraffin-vax precipitates in oil-wells, wellheads and pipelines by the use of biocolloid suspensions
    1.
    发明申请
    Method for the treatment and prevention of asphaltene-paraffin-vax precipitates in oil-wells, wellheads and pipelines by the use of biocolloid suspensions 审中-公开
    通过使用生物胶体悬浮液来处理和预防油井,井口和管道中沥青质 - 石蜡 - 血沉淀物的方法

    公开(公告)号:US20060060350A1

    公开(公告)日:2006-03-23

    申请号:US10530765

    申请日:2003-10-07

    IPC分类号: E21B41/02

    CPC分类号: C12P1/00 B09C1/10 C09K8/524

    摘要: The method for the prevention, reduction and/or removal of asphaltene-paraffin-vax precipitates on surfaces in contact with crude oil within oil-wells from bottom hole to well head, in flow lines and pipelines by the use of biocolloid suspensions comprises the steps of: adding together (a) microorganism(s) capable of reacting with, solubilizing of and/or resistant to the components of crude oil, (b) organic or inorganic additives, or (c) a mixture thereof into the pipes; and allowing the so formed suspension containing the microorganisms to act for a desired time. The suspension is used for the decomposition, washing-off and removal of solid hydrocarbons and mixtures of long chain hydrocarbons, as well as for the prevention of formation thereof.

    摘要翻译: 通过使用生物胶体悬浮液从底孔到井口,流管线和管道中的油井中与原油接触的表面上预防,还原和/或去除沥青质 - 石蜡 - 沉淀物的方法包括以下步骤 (a)能够与原油组分反应,增溶和/或耐受的微生物,(b)有机或无机添加剂或(c)其混合物加入到管道中; 并允许含有微生物的如此形成的悬浮液起作用期望的时间。 悬浮液用于固体烃和长链烃的混合物的分解,洗脱和除去,以及用于防止其形成。

    Petrol-biochemical method for the treatment and prevention of oil-in-water and water-in-oil emulsions in oil-wells and surface equipment
    2.
    发明申请
    Petrol-biochemical method for the treatment and prevention of oil-in-water and water-in-oil emulsions in oil-wells and surface equipment 审中-公开
    用于油井和地面设备中油包水和油包水乳液的处理和预防的汽油生化方法

    公开(公告)号:US20060060527A1

    公开(公告)日:2006-03-23

    申请号:US10530773

    申请日:2003-10-07

    IPC分类号: C02F3/00

    CPC分类号: B09C1/10 B09C1/00 C09K8/524

    摘要: The method for preventing the formation of oil-win-water and/or water-in-oil emulsions and/or for breaking up emulsions already formed, comprises the steps of: (a) adding tensides, materials for increasing viscosity, industrial surfactants, and microorganisms capable of breaking down crude oil components or derivatives and producing at least one type of tenside, to the emulsion already formed or into the device containing the crude oil in which the formation of the emulsion to be prevented, optionally together with additives required for the reproduction of said microorganisms; (b) providing an appropriate temperature for the microorganisms after the addition of the materials listed above; (c) allowing the microorganisms to reproduce and act for a predetermined period of time; (d) checking the results of the treatment; and (e) optionally repeating steps (a) to (d) at least once more, preferably at least three more times.

    摘要翻译: 防止形成油 - 水和/或油包水乳液和/或破碎已经形成的乳液的方法包括以下步骤:(a)添加表面活性剂,增加粘度的材料,工业表面活性剂, 以及能够分解原油组分或衍生物并产生至少一种类型的表面活性剂的微生物,已经形成的乳液或含有待预防乳液形成的原油的装置,任选地与所需的添加剂一起 所述微生物的繁殖; (b)在添加上述材料之后为微生物提供适当的温度; (c)允许微生物繁殖并起作用一段预定的时间; (d)检查治疗结果; 和(e)任选地重复步骤(a)至(d)至少一次,优选至少三次以上。

    Artificial gene coding for authentic human serum albumin, use thereof
and method
    4.
    发明授权
    Artificial gene coding for authentic human serum albumin, use thereof and method 失效
    人造基因编码真正的人血清白蛋白,其用途和方法

    公开(公告)号:US5879907A

    公开(公告)日:1999-03-09

    申请号:US884274

    申请日:1992-05-13

    CPC分类号: C07K14/765

    摘要: A structural gene coding for authentic human serum albumin, --optionally supplemented by an upstream triplet coding for methionine and optionally extended by a synthetic prepro.sup.x -leader-coding sequence--, wherein the codons of the nucleotide sequence have been selected with regard to a non-human host, e.g. yeast, chosen for expression of authentic human serum albumin, is disclosed.Additionally there is disclosed a method of producing said gene.There are also disclosed a recombinant DNA molecule comprising said strucural gene inserted into a vector, and a host transformed with said recombinant DNA molecule.Furthermore there are disclosed a method of producing authentic human serum albumin, an authentic human serum albumin resulting from said method, and a pharmaceutical composition comprising said resulting human serum albumin.

    摘要翻译: 编码真正的人血清白蛋白的结构基因,可选地用编码甲硫氨酸的上游三联体补充,并任选地通过合成的前引物编码序列扩展,其中核苷酸序列的密码子相对于非 - 人类主机,例如 公开了用于表达真实人血清白蛋白的酵母。 另外公开了一种生产所述基因的方法。 还公开了包含插入载体中的所述结构基因和用所述重组DNA分子转化的宿主的重组DNA分子。 此外,还公开了生产真正的人血清白蛋白,由所述方法得到的可靠的人血清白蛋白的方法和包含所述得到的人血清白蛋白的药物组合物。

    Process for the immobilization of compounds comprising nucleophilic
groups
    6.
    发明授权
    Process for the immobilization of compounds comprising nucleophilic groups 失效
    用于固定包含亲核基团的化合物的方法

    公开(公告)号:US4670390A

    公开(公告)日:1987-06-02

    申请号:US526035

    申请日:1983-08-24

    摘要: The invention relates to a process for the immobilization of compounds comprising nucleophilic groups.According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273.degree.-343.degree. K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260.degree.-313.degree. K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing.

    摘要翻译: 本发明涉及一种固定包含亲核基团的化合物的方法。 根据本发明的方法,包含酰氨基的聚合物在缓冲液中溶胀。 为此,任何在分子中不含氨基,巯基和/或羟基的缓冲液是合适的。 向溶胀的聚合物中加入醌(优选对苯醌)在水混溶性有机溶剂中的溶液,并将混合物在273℃-343℃下活化0.5-48小时,优选24小时。 将未反应的醌用水混溶性有机溶剂的水溶液洗涤,然后用水和/或缓冲液洗涤。 在pH值为3至11之间,优选6至8之间的纯化凝胶中,加入包含亲核基团的化合物的缓冲溶液,将悬浮液在260-313℃下孵育24小时,将凝胶 分离,通过洗涤除去包含亲核基团的非结合化合物。