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    Bacterial host strain
    3.
    发明授权
    Bacterial host strain 有权
    细菌宿主菌株

    公开(公告)号：US09109216B2

    公开(公告)日：2015-08-18

    申请号：US13497987

    申请日：2010-09-23

    申请人： Mark Ellis ,  David Paul Humphreys

    发明人： Mark Ellis ,  David Paul Humphreys

    IPC分类号： C12P21/02 ,  C12N9/52 ,  C12N15/70 ,  C07K16/24

    CPC分类号： C12N1/20 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/55 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19 ,  Y02P20/52

    摘要： A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

    摘要翻译： 包含一个或多个以下突变蛋白酶基因的重组革兰氏阴性细菌细胞：a）突变的Tsp基因，其中突变的Tsp基因编码具有降低的蛋白酶活性的Tsp蛋白或是敲除突变的Tsp基因; b）突变的ptr基因，其中突变的ptr基因编码具有降低的蛋白酶活性的蛋白酶III蛋白质或是敲除突变的ptr基因; 和c）编码具有伴侣活性和降低的蛋白酶活性的DegP蛋白的突变DegP基因; 其中所述细胞与野生型细菌细胞是同基因的，除了突变的Tsp基因和/或突变的ptr基因和/或突变的DegP基因和任选的编码目的蛋白质的多核苷酸序列。

    Behavioral profiles in sourcing and recruiting as part of a hiring process
    5.
    发明授权
    Behavioral profiles in sourcing and recruiting as part of a hiring process 有权
    作为招聘过程的一部分，采购和招聘中的行为概况

    公开(公告)号：US08204778B2

    公开(公告)日：2012-06-19

    申请号：US11771704

    申请日：2007-06-29

    申请人： Jason-Jno Engene Taylor ,  Mark Ellis

    发明人： Jason-Jno Engene Taylor ,  Mark Ellis

    IPC分类号： G06Q10/00

    CPC分类号： G06Q10/1053 ,  G06Q10/063118 ,  G06Q10/0639 ,  G06Q10/06393 ,  G06Q10/10 ,  G06Q30/02 ,  G06Q30/0203

    摘要： A method of selecting potential hires for progression through a hiring process is provided. The method comprises selecting at least one performance indicator for a position, obtaining the at least one performance indicator for a plurality of incumbents in the position, and placing each incumbent in the plurality of incumbents along a performance continuum according to their performance indicator. The method also comprises surveying incumbents from at least both halves of the performance continuum, generating an ideal profile for the position based upon the incumbent survey and the at least one performance indicator of the incumbents, using a survey to generate a candidate profile of a potential hire, comparing the candidate profile of the potential hire with the ideal profile for the position to determine a result for the potential hire, and determining whether to progress the potential hire to a next stage in the hiring process based upon the result.

    摘要翻译： 提供了通过招聘过程选择潜在雇员进行进展的方法。 该方法包括为位置选择至少一个绩效指标，获得位于该位置的多个在职者的至少一个绩效指标，以及根据其绩效指标将每个在职者放置在执行连续统计中。 该方法还包括从绩效连续体的至少两个角度对在职人员进行调查，根据现任调查和现任人员的至少一个绩效指标，为职位创造理想的档案，使用调查来生成潜在的候选人资料 雇用，将潜在雇用的候选人资料与职位的理想档案进行比较，以确定潜在雇用的结果，以及根据结果确定是否将潜在雇用晋升到招聘过程中的下一阶段。

    Method For Producing Proteins
    6.
    发明申请
    Method For Producing Proteins 有权
    生产蛋白质的方法

    公开(公告)号：US20120040401A1

    公开(公告)日：2012-02-16

    申请号：US13203182

    申请日：2010-02-25

    申请人： Mark Ellis ,  Laura Ellen Newnham

    发明人： Mark Ellis ,  Laura Ellen Newnham

    IPC分类号： C12P21/00 ,  C12N1/19 ,  C07K16/46 ,  C12N5/10 ,  C12N15/63 ,  C12N15/64

    CPC分类号： C07K16/00

    摘要： The invention relates to a transcriptionally active recombinant linear polynucleotide encoding a multimeric protein comprising in the following order, a first promoter sequence, a first encoding polynucleotide sequence, a bidirectional regulatory sequence, a second encoding polynucleotide sequence and a second promoter sequence, wherein the first and second encoding polynucleotide sequences are in convergent transcriptional orientation, each encoding polynucleotide sequence encodes a component of the multimeric protein and the bidirectional regulatory sequence is operably linked to the first and second encoding polynucleotide sequences and the multimeric protein is an antibody or fragment thereof and each encoding polynucleotide sequence encodes one or more antibody domains or fragments thereof.

    摘要翻译： 本发明涉及编码多聚蛋白的转录活性重组线性多核苷酸，其包含以下顺序，第一启动子序列，第一编码多核苷酸序列，双向调节序列，第二编码多核苷酸序列和第二启动子序列，其中第一 并且第二编码多核苷酸序列处于会聚转录取向，每个编码多核苷酸序列编码多聚体蛋白的组分，并且双向调节序列可操作地连接到第一和第二编码多核苷酸序列，多聚体蛋白是抗体或其片段，并且每个 编码多核苷酸序列编码一个或多个抗体结构域或其片段。

    SYSTEM AND METHOD FOR DETECTING AND REMEDIATING CONTAMINATION
    7.
    发明申请
    SYSTEM AND METHOD FOR DETECTING AND REMEDIATING CONTAMINATION 有权
    用于检测和补救污染的系统和方法

    公开(公告)号：US20070131864A1

    公开(公告)日：2007-06-14

    申请号：US11548559

    申请日：2006-10-11

    申请人： Mark Ellis ,  Son Le ,  Larry Davis

    发明人： Mark Ellis ,  Son Le ,  Larry Davis

    IPC分类号： G01J5/02

    CPC分类号： G01N21/05 ,  G01N21/3504 ,  G01N21/6428 ,  G01N21/783

    摘要： A system for detecting and remediating a discharge of fuel from a fuel source facility is disclosed. The system can comprise a contamination detection subsystem configured to detect a discharge of fuel from the fuel source facility and a remediation subsystem configured to remediate contamination due to the discharge of fuel. A controller can automatically activate the remediation subsystem upon detection of a discharge by the contamination detection subsystem. The controller can automatically send a notification that a contamination has been detected and the remediation system has been activated. Alternatively or in addition, the controller can automatically send a notification that a contamination has been detected and the remediation system has been activated.

    摘要翻译： 公开了一种用于从燃料源设备检测和补救燃料排放的系统。 该系统可以包括被配置为检测来自燃料源设备的燃料排放物的污染检测子系统和被配置为修复由于燃料排放引起的污染物的修复子系统。 检测到污染检测子系统的放电时，控制器可以自动激活补救子系统。 控制器可以自动发送已经检测到污染的通知，并且修复系统已被激活。 或者或另外，控制器可以自动发送已经检测到污染并且补救系统已被激活的通知。

    Behavioral profiles in sourcing and recruiting as part of a hiring process
    9.
    发明授权
    Behavioral profiles in sourcing and recruiting as part of a hiring process 有权
    作为招聘过程的一部分，采购和招聘中的行为概况

    公开(公告)号：US08484072B1

    公开(公告)日：2013-07-09

    申请号：US13460117

    申请日：2012-04-30

    申请人： Jason-Jno Eugene Taylor ,  Mark Ellis

    发明人： Jason-Jno Eugene Taylor ,  Mark Ellis

    IPC分类号： G06Q10/00

    CPC分类号： G06Q10/1053 ,  G06Q10/063118 ,  G06Q10/0639 ,  G06Q10/06393 ,  G06Q10/10 ,  G06Q30/02 ,  G06Q30/0203

    摘要： A method of selecting potential hires for progression through a hiring process is provided. The method comprises selecting at least one performance indicator for a position, obtaining the at least one performance indicator for a plurality of incumbents in the position, and placing each incumbent in the plurality of incumbents along a performance continuum according to their performance indicator. The method also comprises surveying incumbents from at least both halves of the performance continuum, generating an ideal profile for the position based upon the incumbent survey and the at least one performance indicator of the incumbents, using a survey to generate a candidate profile of a potential hire, comparing the candidate profile of the potential hire with the ideal profile to determine a result for the potential hire, and determining whether to progress the potential hire to a next stage in the hiring process based upon the result.

    摘要翻译： 提供了通过招聘过程选择潜在雇员进行进展的方法。 该方法包括为位置选择至少一个绩效指标，获得位于该位置的多个在职者的至少一个绩效指标，以及根据其绩效指标将每个在职者放置在执行连续统计中。 该方法还包括从绩效连续体的至少两个角度对在职人员进行调查，根据现任调查和现任人员的至少一个绩效指标，为职位创造理想的档案，使用调查来生成潜在的候选人资料 雇用，将潜在雇用的候选人资料与理想档案进行比较，以确定潜在雇用的结果，以及根据结果确定是否将招聘过程中的潜在雇用晋升到下一阶段。