公开(公告)号：US20170067076A1

公开(公告)日：2017-03-09

申请号：US15348839

申请日：2016-11-10

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

IPC: C12N15/82 ,  C12N15/113

Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

Abstract translation: 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

公开(公告)号：US20210207155A1

公开(公告)日：2021-07-08

申请号：US17206113

申请日：2021-03-18

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Larry A. Gilbertson ,  Sergey I. Ivashuta ,  James K. Roberts

IPC: C12N15/82

Abstract: This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.

公开(公告)号：US10301623B2

公开(公告)日：2019-05-28

申请号：US15052062

申请日：2016-02-24

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Liang Guo ,  Sara E. Heisel ,  Sergey I. Ivashuta ,  Yuanji Zhang

IPC: C12N15/82 ,  C12N15/113

Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.

公开(公告)号：US09528121B2

公开(公告)日：2016-12-27

申请号：US13746500

申请日：2013-01-22

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

IPC: A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  A01N63/02 ,  C12N15/113

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C12N15/113 ,  C12N15/8285 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531 ,  Y02A40/162 ,  Y02A40/164

Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

Abstract translation: 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

公开(公告)号：US20180237793A1

公开(公告)日：2018-08-23

申请号：US15827886

申请日：2017-11-30

Applicant: Monsanto Technology LLC

Inventor： Eric D. Aasen ,  Mark Scott Abad ,  Edwards Allen ,  Thandoni Rao Ambika ,  Veena S. Anil ,  Alice Clara Augustine ,  Stephanie L. Back ,  Pranesh Badami ,  Amarjit Basra ,  Kraig L. Brustad ,  Molian Deng ,  Todd Dezwaan ,  Charles Dietrich ,  Stephen M. Duff ,  Karen K. Gabbert ,  Steve He ,  Bill L. Hendrix ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Balasulojini Karunanandaa ,  Kevin R. Kosola ,  Kempagangaiah Krishnamurthy ,  Ganesh Kumar ,  Saritha V. Kuriakose ,  Shantala Lakkanna ,  Mark E. Leibman ,  Linda Lutfiyya ,  Savitha Madappa ,  Sivalinganna Manjunath ,  Robert J. Meister ,  Bellur Narasimha Prasad ,  Sasha Preuss ,  Qungang Qi ,  Dhanalakshmi Ramachandra ,  Aniruddha Raychaudhuri ,  S. Sangeetha ,  Scott Saracco ,  Chitresh Sharma ,  Vijay K. Sharma ,  Monnanda S. Rajani ,  Padmini Sudarshana ,  Rebecca L. Thompson-Mize ,  Srikanth Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Jingrui Wu ,  Xiaoyun Wu ,  Xiaofeng Sean Yang ,  Qin Zeng ,  Jianmin Zhao

IPC: C12N15/82

CPC classification number: C12N15/8251 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

公开(公告)号：US20160068852A1

公开(公告)日：2016-03-10

申请号：US14945091

申请日：2015-11-18

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Larry A. Gilbertson ,  Sergey I. Ivashuta ,  James K. Roberts

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  C12N15/8216 ,  C12N15/8274 ,  C12N15/8285 ,  C12N15/8286

Abstract: This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.

Abstract translation: 本发明提供了一种通过从来自至少一个sRNA沉默途径基因的DNA的转基因和RNA的DNA同时转录RNA来自动调节对sRNA沉默敏感的转基因的表达的方法。 本发明的一个方面提供了包含sRNA沉默调节子的转基因DNA和DNA的重组DNA构建体的用途。 还公开了在其基因组中具有重组DNA构建体的转基因细胞和生物体，其包含转基因的DNA和sRNA沉默调节子的DNA。

公开(公告)号：US09192112B2

公开(公告)日：2015-11-24

申请号：US13677607

申请日：2012-11-15

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Larry A Gilbertson ,  James K Roberts ,  Nancy M Houmard ,  Shihshieh Huang ,  Sergey Ivashuta

IPC: A01H1/02 ,  C12N15/82 ,  C12N15/113 ,  A01H5/00 ,  A01H5/10

CPC classification number: C12N15/8218 ,  A01H1/02 ,  A01H5/00 ,  A01H5/10 ,  C12N15/8287 ,  C12N15/8289 ,  C12N15/829

Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

Abstract translation: 本发明提供了生产非天然杂种种子的方法。 还公开了可用于在作物植物上赋予诱导型不育性的特异性miRNA和miRNA识别位点，以及包含这种外源miRNA识别位点的重组DNA构建体。

公开(公告)号：US10570414B2

公开(公告)日：2020-02-25

申请号：US15348839

申请日：2016-11-10

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

IPC: C12N15/82 ,  A01N63/02 ,  C12N15/113

Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

公开(公告)号：US20160060648A1

公开(公告)日：2016-03-03

申请号：US14939691

申请日：2015-11-12

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards Allen ,  Veena S. Anil ,  Amarjit Basra ,  Christopher T. Bauer ,  Prasanna R. Bhat ,  Raghunatha P. Chari ,  Jaishree Chittoor ,  Paul Chomet ,  Karen K. Gabbert ,  Cara Griffith ,  Bill Hendrix ,  Alberto Iandolino ,  Hongwu Jia ,  Kevin R. Kosola ,  Saritha V. Kuriakose ,  Paul J. Loida ,  Linda L. Lutfiyya ,  Mingsheng Peng ,  Ying Peng ,  Bellur Narasimha Prasad ,  Monnanda S. Rajani ,  Monica P. Ravanello ,  Donald Todd ,  Srikanth Babu Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  K. R. Vidya ,  Huai Wang

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C07K14/415 ,  C12N15/8271 ,  C12N15/8273

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of Arabidopsis thaliana heat stress transcription factor A-2, which is useful for imparting enhanced agronomic traits) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

Abstract translation: 本发明提供具有用于表达拟南芥热胁迫转录因子A-2的重组DNA的转基因植物细胞，其可用于赋予改良的农艺性状）转基因作物植物。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20130165498A1

公开(公告)日：2013-06-27

申请号：US13746500

申请日：2013-01-22

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

IPC: C12N15/113

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C12N15/113 ,  C12N15/8285 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531 ,  Y02A40/162 ,  Y02A40/164

Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

Abstract translation: 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供了抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种目标基因的方法，包括提供包含本发明的非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。