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公开(公告)号：US11091767B2
公开(公告)日：2021-08-17
申请号：US16516025
申请日：2019-07-18
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, Jr. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells, such as corn plant immature embryo for bacterial-mediated transformation comprising contacting the plant cells, such as corn plant immature embryo with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells, such as corn plant immature embryo for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells, such as corn plant immature embryo in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号：US11473094B2
公开(公告)日：2022-10-18
申请号：US17317255
申请日：2021-05-11
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号：US09648814B2
公开(公告)日：2017-05-16
申请号：US14314963
申请日：2014-06-25
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
CPC classification number: A01H5/00 , A01G7/00 , A01H4/001 , A01H4/003 , A01H4/008 , A01H5/10 , C12N15/82 , C12N15/8201 , C12N15/8205
Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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Patent Agency Ranking
公开(公告)号：US11212972B2
公开(公告)日：2022-01-04
申请号：US16553911
申请日：2019-08-28
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US10918032B2
公开(公告)日：2021-02-16
申请号：US16106342
申请日：2018-08-21
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US11477954B2
公开(公告)日：2022-10-25
申请号：US17124032
申请日：2020-12-16
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US10433503B2
公开(公告)日：2019-10-08
申请号：US15452519
申请日：2017-03-07
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
Abstract: The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US10091957B2
公开(公告)日：2018-10-09
申请号：US14339934
申请日：2014-07-24
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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