公开(公告)号：US10408787B2

公开(公告)日：2019-09-10

申请号：US13976157

申请日：2011-11-30

申请人： Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

发明人： Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

IPC分类号： G01N27/447 ,  G01N30/60

摘要： In an apparatus for analyzing small number of samples, wasteful consumption of a polymer as a separation Medium is suppressed.The apparatus has a configuration provided with a plurality of capillary units each of which can be attached/detached to/from the apparatus, and performs analysis for capillaries after sealing of a polymer by attaching the capillaries by the number in accordance with the number of samples. Since analysis can be performed by using capillaries by the number identical with that of the samples to be analyzed, wasteful polymer is not used in the capillaries not used for the analysis. In addition, the polymer injection mechanism is unnecessary and the polymer is not necessary for maintenance and upon starting of analysis. An electrophoresis apparatus which is small in the size with no polymer injection mechanism, and with low running cost not wastefully consuming the polymer can be provided.

公开(公告)号：US20130292251A1

公开(公告)日：2013-11-07

申请号：US13976157

申请日：2011-11-30

申请人： Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

发明人： Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

IPC分类号： G01N27/447

摘要： In an apparatus for analyzing small number of samples, wasteful consumption of a polymer as a separation Medium is suppressed.The apparatus has a configuration provided with a plurality of capillary units each of which can be attached/detached to/from the apparatus, and performs analysis for capillaries after sealing of a polymer by attaching the capillaries by the number in accordance with the number of samples. Since analysis can be performed by using capillaries by the number identical with that of the samples to be analyzed, wasteful polymer is not used in the capillaries not used for the analysis. In addition, the polymer injection mechanism is unnecessary and the polymer is not necessary for maintenance and upon starting of analysis. An electrophoresis apparatus which is small in the size with no polymer injection mechanism, and with low running cost not wastefully consuming the polymer can be provided.

摘要翻译： 在分析少量样品的装置中，抑制作为分离介质的聚合物的浪费的消耗。 该装置具有设置有多个毛细管单元的构造，每个毛细管单元可以与该装置相连接/分离，并且在密封聚合物之后对毛细管进行分析，该毛细管通过根据样品数量附加毛细管数量 。 由于可以通过使用与要分析的样品相同数量的毛细管进行分析，所以在不用于分析的毛细管中不使用浪费的聚合物。 此外，聚合物注入机制是不必要的，并且聚合物不需要维护和分析开始时。 可以提供一种尺寸小且无聚合物注射机制，并且运行成本低，不浪费地消耗聚合物的电泳装置。

公开(公告)号：US07883613B2

公开(公告)日：2011-02-08

申请号：US11806889

申请日：2007-06-05

申请人： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

发明人： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

IPC分类号： G01N27/453

CPC分类号： G01N27/44721

摘要： The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.

摘要翻译： 本发明提供了一种毛细管电泳装置，其中毛细管容易地连接到迁移介质填充单元并从迁移介质填充单元中分离而不将杂质混入毛细管中。 当毛细管负极端与存储在容器中的样品接触时，也可以防止诸如灰尘等杂质的混合物。 此外，在毛细管中有效地进行温度控制。 在毛细管电泳装置中，通过用手抓握把手部分可以支撑整个毛细管阵列。 迁移介质填充机构包括相对于毛细管头移动聚合物嵌段的滑动机构。 毛细管电泳装置包括可以同时存储样品和缓冲液的容器。 毛细管和照射和检测单元的温度由恒温装置中提供的温度控制功能控制。

公开(公告)号：US20080217177A1

公开(公告)日：2008-09-11

申请号：US12073296

申请日：2008-03-04

申请人： Ryoji Inaba ,  Motohiro Yamazaki ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

发明人： Ryoji Inaba ,  Motohiro Yamazaki ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

IPC分类号： G01N27/26

CPC分类号： G01N27/447

摘要： There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed.Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.

摘要翻译： 提供了一种毛细管电泳装置，其中激光照射光与毛细管阵列的耦合效率即使去除两个毛细管阵列的任何一个毛细管阵列也不会下降。 通过一个光学检测系统检测由一个辐射来自一侧的激发光的2μs（n是正整数）毛细管阵列的毛细管阵列的毛细管产生的发光。

公开(公告)号：US20060231400A1

公开(公告)日：2006-10-19

申请号：US11397890

申请日：2006-04-05

申请人： Ryoji Inaba ,  Tomoyuki Sakai ,  Motohiro Yamazaki ,  Takashi Gomi ,  Kazumichi Imai

发明人： Ryoji Inaba ,  Tomoyuki Sakai ,  Motohiro Yamazaki ,  Takashi Gomi ,  Kazumichi Imai

IPC分类号： B01D57/02 ,  G01N27/00

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.

公开(公告)号：US07787120B2

公开(公告)日：2010-08-31

申请号：US12146920

申请日：2008-06-26

申请人： Motohiro Yamazaki ,  Ryoji Inaba ,  Kosaku Toyosaki ,  Shintaro Kubo ,  Takashi Gomi ,  Katsunobu Hama ,  Kiyotoshi Mori ,  Hideyuki Akiyama

发明人： Motohiro Yamazaki ,  Ryoji Inaba ,  Kosaku Toyosaki ,  Shintaro Kubo ,  Takashi Gomi ,  Katsunobu Hama ,  Kiyotoshi Mori ,  Hideyuki Akiyama

IPC分类号： G01J3/10 ,  G01J3/36 ,  G01N21/33

CPC分类号： G01J3/10 ,  G02B5/283 ,  G02B27/1006 ,  G02B27/1086 ,  G02B27/145 ,  G02B27/148

摘要： Detection sensitivity is improved by increasing the amount of light of beams that irradiate a sample cell without causing saturation of a detector with ultraviolet beams or visible beams. This spectrophotometer includes a sample cell, which stores a sample to be measured, a visible light source and an ultraviolet light source each for supplying an incident beam that enters the sample, a spectroscope, which disperses a beam that has passed through the sample, an optical detector, which detects beams dispersed from such beam (spectrum), and a dichroic element which reflects or transmits ultraviolet beams from the ultraviolet light source and which transmits or reflects visible beams from the visible light source. Optics are configured such that ultraviolet beams and visible beams that have passed through or have been reflected by the dichroic element enter the sample cell.

摘要翻译： 通过增加照射样品池的光束的光量而不会使紫外线束或可见光束的检测器饱和而提高检测灵敏度。 该分光光度计包括存储待测样品，可见光源和紫外光源的样品池，每个用于提供进入样品的入射光束，分散已经通过样品的光束的分光器， 检测从这种光束（光谱）分散的光束的光检测器，以及从紫外光源反射或透射紫外光束并从可见光源透射或反射可见光束的二向色元件。 光学构造使得已经通过或已被二色性元件反射的紫外光束和可见光束进入样品池。

公开(公告)号：US08545686B2

公开(公告)日：2013-10-01

申请号：US11397890

申请日：2006-04-05

申请人： Ryoji Inaba ,  Tomoyuki Sakai ,  Motohiro Yamazaki ,  Takashi Gomi ,  Kazumichi Imai

发明人： Ryoji Inaba ,  Tomoyuki Sakai ,  Motohiro Yamazaki ,  Takashi Gomi ,  Kazumichi Imai

IPC分类号： G01N27/453 ,  G01N27/447

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.

摘要翻译： 本发明涉及通过将激发光照射到多个电泳路径上并且将电泳路径输出的荧光以大致垂直于电泳方向的方向分散的方式来检测发射光谱。 根据本发明，由于要检测的发射光谱随时间基本上不变化，因此可以使观察到的发射光谱完全对应于各种荧光染料或各种基底。

公开(公告)号：US08262888B2

公开(公告)日：2012-09-11

申请号：US12073296

申请日：2008-03-04

申请人： Ryoji Inaba ,  Motohiro Yamazaki ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

发明人： Ryoji Inaba ,  Motohiro Yamazaki ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/447

摘要： There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed.Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.

摘要翻译： 提供了一种毛细管电泳装置，其中激光照射光与毛细管阵列的耦合效率即使去除两个毛细管阵列的任何一个毛细管阵列也不会下降。 通过一个光学检测系统检测由辐射来自一侧的激发光的2n（n是正整数）毛细管阵列的毛细管阵列的毛细管产生的发光。

公开(公告)号：US07473342B2

公开(公告)日：2009-01-06

申请号：US11395158

申请日：2006-04-03

申请人： Seiichi Ugai ,  Tomonari Morioka ,  Ryoji Inaba ,  Akihiro Suzuki ,  Yoshinori Ookoshi ,  Yoshiyuki Okishima ,  Tsukasa Ohira ,  Motohiro Yamazaki ,  Takashi Gomi

发明人： Seiichi Ugai ,  Tomonari Morioka ,  Ryoji Inaba ,  Akihiro Suzuki ,  Yoshinori Ookoshi ,  Yoshiyuki Okishima ,  Tsukasa Ohira ,  Motohiro Yamazaki ,  Takashi Gomi

IPC分类号： G01N27/00

CPC分类号： G01N27/44708

摘要： In a case where the temperature of capillaries cannot be controlled rapidly in accordance with the change of the amount of heat generated from the capillaries, the phoretic speed of the sample is not controlled constant to lower the analyzing performance for the sample. This invention intends to stabilize the electrophoretic speed. The surface of a substrate on which a plurality of capillaries are arranged is in contact with a member capable of temperature control thereby controlling the temperature of the capillaries. Since a member capable of controlling the temperature and the capillaries are in direct contact with each other according to the invention, the temperature of the capillary can be controlled rapidly at a high accuracy, so that electrophoretic speed is stabilized.

摘要翻译： 在毛细管的温度不能根据毛细管产生的热量的变化而不能快速控制的情况下，不将样品的凝胶速度控制在一定程度以降低样品的分析性能。 本发明旨在稳定电泳速度。 其上布置有多个毛细管的基板的表面与能够进行温度控制的构件接触，从而控制毛细管的温度。 由于根据本发明能够控制温度和毛细管的构件彼此直接接触，所以可以高精度地快速地控制毛细管的温度，使得电泳速度稳定。

公开(公告)号：US20080110756A1

公开(公告)日：2008-05-15

申请号：US11979570

申请日：2007-11-06

申请人： Motohiro Yamazaki ,  Ryoji Inaba ,  Satoshi Takahashi ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

发明人： Motohiro Yamazaki ,  Ryoji Inaba ,  Satoshi Takahashi ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

IPC分类号： B01D57/02

CPC分类号： B01D57/02 ,  C07K1/26 ,  G01N27/44721 ,  G01N27/44782

摘要： The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased.An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.

摘要翻译： 本发明的目的是在电泳单元中扩展动态范围，同时增加多个样本之间的浓度差。 在分析过程中调整样品的照射时间，而不改变采样时间。 通过缩短照射时间，减少样品的荧光量，使得由检测器检测出的信号强度物理降低。 如果照射时间非常短（几百毫秒），照射时间和荧光强度成正比关系。 已知如果照射时间减少到1 / n，则荧光强度即被检测信号强度为1 / n。 因此，对于在分析期间照射时间减少到1 / n的数据，将通过将基本上测量的值乘以n获得的数据用作数据分析，作为最初获取的真实值。