Transgenic plants with elevated thioredoxin levels
    1.
    发明授权
    Transgenic plants with elevated thioredoxin levels 失效
    具有升高的硫氧还蛋白水平的转基因植物

    公开(公告)号:US07179964B2

    公开(公告)日:2007-02-20

    申请号:US10194885

    申请日:2002-07-12

    IPC分类号: C12N15/82 A01H5/00

    摘要: The present invention is directed to a transgenic plant wherein at least a part of said plant includes a recombinant nucleic acid with a promoter active in the part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein the promoter is a seed or grain maturation-specific promoter and the thioredoxin polypeptide includes the amino acid sequence WCGPC. The present invention is further directed to transgenic plants that overexpress thioredoxin in seed wherein the overexpression of thioredoxin h effects a significant increase in the reduction of proteins (—SH as compared to S—S) of the albumin fraction of the seed.

    摘要翻译: 本发明涉及一种转基因植物,其中所述植物的至少一部分包括具有启动子活性的启动子的重组核酸,其与编码硫氧还蛋白多肽的核酸可操作地连接,其中所述启动子是种子或谷物成熟特异性 启动子和硫氧还蛋白多肽包括氨基酸序列WCGPC。 本发明进一步涉及在种子中过表达硫氧还蛋白的转基因植物,其中硫氧还蛋白的过表达h与种子的白蛋白部分的蛋白质的降低(-SH)相比显着增加。

    Compositions and methods for plant transformation and regeneration
    7.
    发明授权
    Compositions and methods for plant transformation and regeneration 失效
    植物转化和再生的组成和方法

    公开(公告)号:US06235529B1

    公开(公告)日:2001-05-22

    申请号:US08845939

    申请日:1997-04-29

    IPC分类号: C12N504

    CPC分类号: A01H4/005

    摘要: Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.

    摘要翻译: 公开了用于从胚性愈伤组织转化和再生植物的改进的组合物和方法,其包括例如:在愈伤组织诱导后使用中间培养培养基以增加转化细胞的再生能力; 选择早期阶段的昏暗条件; 使用绿色愈伤组织作为微粒轰击的目标; 和具有优化水平的植物激素和铜浓度的培养基。

    Compositions and methods for plant transformation and regeneration
    9.
    发明授权
    Compositions and methods for plant transformation and regeneration 有权
    植物转化和再生的组成和方法

    公开(公告)号:US07102056B1

    公开(公告)日:2006-09-05

    申请号:US09552252

    申请日:2000-04-18

    CPC分类号: C12N15/8207 C12N15/8206

    摘要: Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.

    摘要翻译: 公开了用于从胚性愈伤组织转化和再生植物的改进的组合物和方法,其包括例如:在愈伤组织诱导后使用中间培养培养基以增加转化细胞的再生能力; 选择早期阶段的昏暗条件; 使用绿色愈伤组织作为微粒轰击的目标; 和具有优化水平的植物激素和铜浓度的培养基。

    Method for preparing barley green regenerative tissue
    10.
    发明授权
    Method for preparing barley green regenerative tissue 失效
    制备大麦绿色再生组织的方法

    公开(公告)号:US06541257B2

    公开(公告)日:2003-04-01

    申请号:US09825217

    申请日:2001-04-03

    IPC分类号: C12N504

    CPC分类号: A01H4/005

    摘要: A method of preparing green regenerative tissue of barley suitable for transformation is presented. The method includes incubating barley tissue on a callus induction medium under dim light. The dim light conditions are sufficient to produce green regenerative tissue. The callus induction media includes auxin and copper.

    摘要翻译: 提出了一种制备适合转化的大麦绿色再生组织的方法。 该方法包括在暗光下在愈伤组织诱导培养基上培养大麦组织。 昏暗的光线条件足以产生绿色再生组织。 愈伤组织诱导培养基包括生长素和铜。