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40 条记录 (耗时 0.016 秒)

    Construction of barley with reduced gel protein content
    1.
    发明授权
    Construction of barley with reduced gel protein content 失效
    建立具有降低凝胶蛋白含量的大麦

    公开(公告)号：US07074986B1

    公开(公告)日：2006-07-11

    申请号：US10048987

    申请日：2000-08-16

    申请人： Naohiko Hirota ,  Makoto Kihara ,  Kazutoshi Ito

    发明人： Naohiko Hirota ,  Makoto Kihara ,  Kazutoshi Ito

    IPC分类号： A01H5/00 ,  C12N15/82

    CPC分类号： C12N15/8242 ,  C07K14/415 ,  C12N15/8251

    摘要： In barley, the formation of D-hordein constituting gel protein is to be reduced. This reduction in the formation of D-hordein causes a reduction of gel protein because D-hordein fraction decreases in the gel protein; and in addition, it brings about the transformation (reduction) of proteins other than D-hordein which constitute the gel protein from the gel protein. Therefore, by introducing into barley D-hordein expression suppressing vector 1 capable of suppressing the D-hordein formation and transforming the barley therewith, it is possible to attain the reduction of gel protein based on the reduction in the D-hordein formation.

    摘要翻译： 在大麦中，减少构成凝胶蛋白的D-大麦醇溶蛋白的形成。 这种形成D-大麦醇溶蛋白的减少导致凝胶蛋白质的减少，因为D-大麦醇溶蛋白部分在凝胶蛋白中降低; 此外，还引起了来自凝胶蛋白质的构成凝胶蛋白质的除了大麦醇溶蛋白以外的蛋白质的转化（降低）。 因此，通过将能够抑制D-大麦芽糖苷形成和大麦转化的大麦醇溶蛋白表达抑制载体1导入，可以基于D-大麦醇溶蛋白形成的降低而获得凝胶蛋白质的还原。

    Gene expression regulatory DNA, expression cassette, expression vector and transgenic plant
    2.
    发明授权
    Gene expression regulatory DNA, expression cassette, expression vector and transgenic plant 失效
    基因表达调控DNA，表达盒，表达载体和转基因植物

    公开(公告)号：US5955649A

    公开(公告)日：1999-09-21

    申请号：US899336

    申请日：1997-07-23

    申请人： Naohiko Hirota ,  Makoto Kihara ,  Hisao Kuroda ,  Kazutoshi Ito

    发明人： Naohiko Hirota ,  Makoto Kihara ,  Hisao Kuroda ,  Kazutoshi Ito

    IPC分类号： A01H5/00 ,  C07K14/415 ,  C12N5/10 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  C12N5/04

    CPC分类号： C07K14/415 ,  C12N15/8234

    摘要： The present invention provides new gene expression regulatory DNA. This gene expression regulatory DNA comprises a promotor region derived from the barley D-hordein gene enabling the expression of a desired gene, and a regulatory region for regulating the expression of said gene based on said promoter region. This regulatory region consists at least of an activating region to activate the expression of said gene and a suppressing region to suppress the same.Use of this gene expression regulatory DNA enables controlling as desired the expression of gene within a plant cell such as barley.

    摘要翻译： 本发明提供新的基因表达调控DNA。 该基因表达调控DNA包含来源于能够表达所需基因的大麦D-大麦尔多蛋白基因的启动子区域，以及用于基于所述启动子区域调节所述基因表达的调节区域。 该调节区域至少包含激活所述基因表达的活化区域和抑制基因的抑制区域。 使用这种基因表达调控DNA可以根据需要控制植物细胞如大麦中基因的表达。

    Method for identifying a barley variety and a barley having a brewing property
    5.
    发明授权
    Method for identifying a barley variety and a barley having a brewing property 失效
    识别大麦品种的方法和具有酿造性能的大麦

    公开(公告)号：US06492576B2

    公开(公告)日：2002-12-10

    申请号：US09104536

    申请日：1998-06-26

    申请人： Makoto Kihara ,  Takafumi Kaneko ,  Kensuke Fukuda ,  Kazutoshi Ito

    发明人： Makoto Kihara ,  Takafumi Kaneko ,  Kensuke Fukuda ,  Kazutoshi Ito

    IPC分类号： A01H500

    CPC分类号： C12N9/2411 ,  A01H1/04 ,  A01H5/10 ,  C12N9/2422 ,  C12Q1/40 ,  G01N33/68 ,  G01N2333/415 ,  G01N2333/926

    摘要： A method for identifying barley with good brewing properties using the thermostability of the barley &bgr;-amylase as an indicator. The thermostability of the barley &bgr;-amylase significantly affects the degree of the apparent attenuation limit. A method for determining the enzyme activity of a extract solution from one barley seed, an indirect method by an isoelectric point, and an indirect identifying method by DNA polymorphisms of the region containing the &bgr;-amylase structural gene have been developed as a method for determining the type of thermostability for a barley &bgr;-amylase. The selection method is not affected by environmental or climatic conditions.

    摘要翻译： 使用大麦β-淀粉酶的热稳定性作为指标来鉴定具有良好酿造性质的大麦的方法。 大麦β-淀粉酶的热稳定性显着影响表观衰减极限的程度。 已经开发了确定来自一个大麦种子的提取物溶液的酶活性，通过等电点的间接方法以及通过含有β-淀粉酶结构基因的区域的DNA多态性的间接鉴定方法的方法作为测定方法 大麦β-淀粉酶的热稳定性类型。 选择方法不受环境或气候条件的影响。

    PROCESSED BARLEY PRODUCT AND PROCESSING METHOD THEREFOR
    6.
    发明申请
    PROCESSED BARLEY PRODUCT AND PROCESSING METHOD THEREFOR 审中-公开
    加工的BARLEY产品及其加工方法

    公开(公告)号：US20090208631A1

    公开(公告)日：2009-08-20

    申请号：US12293964

    申请日：2007-03-22

    申请人： Makoto Kihara ,  Kazutoshi Ito ,  Brian G. Rossnagel

    发明人： Makoto Kihara ,  Kazutoshi Ito ,  Brian G. Rossnagel

    IPC分类号： A23L2/38 ,  A21D6/00

    CPC分类号： A23L33/175 ,  A23F3/34 ,  A23L2/38 ,  A23L7/198 ,  A23V2002/00 ,  C12H6/02 ,  A23V2250/038

    摘要： The object of the present invention is to provide processed barley products which are rich in GABA even after milling of barley seeds, and a processing method therefore. The present invention provides a method for processing barley comprising a step in which barley seeds, whose GABA residue when processed to a pearling degree of 60% is 50% or greater compared to that of the unprocessed seeds, are milled without germination.

    摘要翻译： 本发明的目的是提供即使在研磨大麦种子之后也富含GABA的加工大麦制品，因此加工方法。 本发明提供了一种加工大麦的方法，该方法包括以下步骤：其中加工成具有60％的珠光度的GABA残余物的大麦种子与未加工的种子相比为50％或更大，其中GABA残留物没有发芽而被研磨。