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5 条记录 (耗时 0.03 秒)

    Cyclic depsipeptide synthetase and method for recombinant production
    1.
    发明授权
    Cyclic depsipeptide synthetase and method for recombinant production 失效
    循环depipipeptide合成酶和重组生产方法

    公开(公告)号：US07285404B1

    公开(公告)日：2007-10-23

    申请号：US10070387

    申请日：2000-09-07

    申请人： Naoki Midoh ,  Kaoru Okakura ,  Koichi Miyamoto ,  Manabu Watanabe ,  Koji Yanai ,  Tetsuya Yasutake ,  Sato Aihara ,  Takafumi Futamura ,  Horst Kleinkauf ,  Takeshi Murakami

    发明人： Naoki Midoh ,  Kaoru Okakura ,  Koichi Miyamoto ,  Manabu Watanabe ,  Koji Yanai ,  Tetsuya Yasutake ,  Sato Aihara ,  Takafumi Futamura ,  Horst Kleinkauf ,  Takeshi Murakami

    IPC分类号： C12N9/00

    CPC分类号： C12N9/00 ,  C12P21/00

    摘要： The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.

    摘要翻译： 本发明提供合成环状缩肽，特别是物质1022及其基因的酶。 根据本发明的循环倍肽合成酶包含（a）SEQ ID NO：2的氨基酸序列或（b）SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列，其具有一个或多个修饰选择 从取代，缺失，添加和插入，并具有循环衰老肽合成酶活性。 根据本发明的环状缩肽酶合成酶基因包含编码环状倍肽合成酶的核苷酸序列。 本发明还提供了用于表达环状缩酚酸肽合成酶的重组载体和转化体，以及用于环状缩酚酸肽的批量生产系统。 本发明还提供了一种用于制备环状缩酚酸肽合成酶的方法。

    (R)-2-hydroxy-3-phenylpropionate (d-phenyllactate) dehydrogenase and gene encoding the same
    2.
    发明授权
    (R)-2-hydroxy-3-phenylpropionate (d-phenyllactate) dehydrogenase and gene encoding the same 失效
    （R）-2-羟基-3-苯基丙酸酯（d-苯基乳酸）脱氢酶及其编码基因

    公开(公告)号：US06916641B2

    公开(公告)日：2005-07-12

    申请号：US10258472

    申请日：2001-04-26

    申请人： Koichi Miyamoto ,  Naomi Sumida ,  Naoki Midoh ,  Takeshi Murakami ,  Rainer Zocher ,  Horst Kleinkauf

    发明人： Koichi Miyamoto ,  Naomi Sumida ,  Naoki Midoh ,  Takeshi Murakami ,  Rainer Zocher ,  Horst Kleinkauf

    IPC分类号： C12N1/21 ,  C12N9/02 ,  C12P7/42 ,  C12N9/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04

    CPC分类号： C12N9/0008 ,  C12P7/42

    摘要： An objective of the present invention is to provide a D-phenyllactic acid dehydrogenase. Another objective of the present invention is to provide a gene which encodes the D-phenyllactic acid dehydrogenase. A D-phenyllactic acid dehydrogenase according to the present invention is a protein comprising an amino acid sequence of the amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that has one or more modifications selected from a substitution, a deletion, an addition and an insertion and has D-phenyllactic acid dehydrogenase activity. A D-phenyllactic acid dehydrogenase gene according to the present invention comprises a nucleotide sequence which encodes the D-phenyllactic acid dehydrogenase, for example the nucleotide sequence of SEQ ID NO: 1.

    摘要翻译： 本发明的目的是提供D-苯基乳酸脱氢酶。 本发明的另一个目的是提供编码D-苯基乳酸脱氢酶的基因。 根据本发明的D-苯基乳酸脱氢酶是包含SEQ ID NO：2的氨基酸序列的氨基酸序列或SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列的蛋白质，其具有一个 或更多选自取代，缺失，添加和插入的修饰，并具有D-苯基乳酸脱氢酶活性。 根据本发明的D-苯基乳酸脱氢酶基因包含编码D-苯基乳酸脱氢酶的核苷酸序列，例如SEQ ID NO：1的核苷酸序列。