公开(公告)号：US20130281404A1

公开(公告)日：2013-10-24

申请号：US13824518

申请日：2011-09-26

申请人： Kazunaga Yazawa ,  Tomoyuki Susa ,  Shoji Gotoh ,  Yasuhito Tashiro ,  Junichi Kawashima ,  Shigeyuki Imamura

发明人： Kazunaga Yazawa ,  Tomoyuki Susa ,  Shoji Gotoh ,  Yasuhito Tashiro ,  Junichi Kawashima ,  Shigeyuki Imamura

IPC分类号： C12P13/06 ,  A61K31/685

CPC分类号： C12P13/06 ,  A61K31/685 ,  C12P7/6481

摘要： The present invention provides a method for producing a composition containing 2-acyl-lysophosphatidylserine, including (a) a step of obtaining a composition containing phosphatidylserine by allowing phospholipase D to act on a raw material containing phosphatidylcholine in the presence of serine and (b) a step of obtaining a composition containing 2-acyl-lysophosphatidylserine by allowing phospholipase A1 to act on the composition containing phosphatidylserine in the presence of one or more additives selected from the group consisting of the following (I), (II), and (III), in which (I) one or more salts selected from the group consisting of sulfate salts, phosphate salts, nitrate salts, citrate salts, and tartarate salts of monovalent cations, (II) a gum, and (III) an emulsifier.

摘要翻译： 本发明提供一种含有2-酰基 - 溶血磷脂酰丝氨酸的组合物的制造方法，其包括（a）在丝氨酸存在下使磷脂酶D作用于含有磷脂酰胆碱的原料，得到含有磷脂酰丝氨酸的组合物的工序，（b） 在一种或多种选自以下（I），（II）和（III）中的添加剂的存在下，通过使磷脂酶A1作用于含有磷脂酰丝氨酸的组合物，获得含有2-酰基 - 溶血磷脂酰丝氨酸的组合物的步骤 ），其中（I）选自一价阳离子的硫酸盐，磷酸盐，硝酸盐，柠檬酸盐和酒石酸盐中的一种或多种盐，（II）树胶和（III）乳化剂。

公开(公告)号：US5801178A

公开(公告)日：1998-09-01

申请号：US597983

申请日：1996-02-07

申请人： Kazunaga Yazawa ,  Kazuo Watanabe ,  Yasuharu Ijuin ,  Mayumi Shikano ,  Yasuji Soda ,  Tetsuya Kosaka ,  Naoto Matsuyama ,  Koji Mizuno

发明人： Kazunaga Yazawa ,  Kazuo Watanabe ,  Yasuharu Ijuin ,  Mayumi Shikano ,  Yasuji Soda ,  Tetsuya Kosaka ,  Naoto Matsuyama ,  Koji Mizuno

IPC分类号： C07C233/09 ,  C07C233/15 ,  C07C233/27 ,  C07C233/38 ,  C07C275/28 ,  C07C275/40 ,  C07D295/18 ,  A61K31/495 ,  A61K31/165 ,  C07D295/185

CPC分类号： C07C233/38 ,  C07C233/09 ,  C07C233/15 ,  C07C233/27 ,  C07C275/28 ,  C07C275/40 ,  C07D295/18

摘要： An aniline derivative of the formula (1) ##STR1## wherein R.sup.1 is an eicosapentaenoyl or docosahexaenoyl; R.sup.2 and R.sup.3 are each independently an alkyl or alkoxy having 1 to 6 carbon atoms, or a halogen atom; R.sup.4 is a hydrogen atom, an alkyl or alkoxy having 1 to 6 carbon atoms, or a halogen atom; and A is a single bond, --C(.dbd.O)NH--(CH.sub.2).sub.n --NH-- wherein n is 2 or 3, or a bivalent group of the following formula ##STR2## wherein m and p are each independently 0 or 1. The aniline derivative of the present invention has high inhibitory activity against and high selectivity for ACAT derived from macrophage and is useful as an agent for the prophylaxis and treatment of arteriosclerosis.

摘要翻译： 式（1）的苯胺衍生物其中R 1是二十碳五烯酰基或二十二碳六烯酰基; R2和R3各自独立地为具有1至6个碳原子的烷基或烷氧基或卤素原子; R4是氢原子，具有1至6个碳原子的烷基或烷氧基或卤素原子; 并且A是单键，-C（= O）NH-（CH 2）n -NH-，其中n是2或3，或下式的二价基团，其中m和p各自独立地 0或1.本发明的苯胺衍生物对来自巨噬细胞的ACAT具有高的抑制活性和高选择性，可用作预防和治疗动脉硬化的药剂。

公开(公告)号：US5798259A

公开(公告)日：1998-08-25

申请号：US752929

申请日：1996-11-20

申请人： Kazunaga Yazawa ,  Akiko Yamada ,  Seishi Kato ,  Kiyosi Kondo

发明人： Kazunaga Yazawa ,  Akiko Yamada ,  Seishi Kato ,  Kiyosi Kondo

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12P7/64 ,  C12N15/52

CPC分类号： C12P7/6472 ,  C12N9/93 ,  C12P7/6427

摘要： There is provided an advantageous process for production of EPA by a gene recombinant technique wherein genes coding for biosynthesis enzymes for eicosapentaenoic acid (EPA) useful as pharmaceuticals, agrochemicals, foods, feeds or the like is obtained from microorganisms. EPA is produced by obtaining genes coding for eicosapentaenoic acid (EPA) biosynthesis enzymes, constructing a plasmid by joining the genes to a vector, transforming E. coli with the plasmid, and culturing the transformed E. coli.

摘要翻译： 提供了通过基因重组技术生产EPA的有利方法，其中可从微生物获得编码二十碳五烯酸（EPA）的生物合成酶的基因，其用作药物，农业化学品，食品，饲料等。 EPA通过获得编码二十碳五烯酸（EPA）生物合成酶的基因产生，通过将基因连接到载体构建质粒，用质粒转化大肠杆菌，并培养转化的大肠杆菌。

公开(公告)号：US5436269A

公开(公告)日：1995-07-25

申请号：US87708

申请日：1993-11-08

申请人： Kazunaga Yazawa ,  Tomohito Hamasaki ,  Hirofumi Taki ,  Kiyosi Kondo ,  Mitihiro Sugano ,  Ikuo Ikeda

发明人： Kazunaga Yazawa ,  Tomohito Hamasaki ,  Hirofumi Taki ,  Kiyosi Kondo ,  Mitihiro Sugano ,  Ikuo Ikeda

IPC分类号： A61K31/20 ,  A61K31/225

CPC分类号： A61K31/20

摘要： A drug for hepatic diseases, particularly an ameliorant for hepatic functions and a drug for hepatitis, containing docosahexaenoic acid or a derivative thereof (DHA) as an active ingredient. The DHA as the active drug ingredient, though also taking part in the metabolism of cholesterol formed in vivo and exuding into the plasma, mainly metabolizes that cholesterol specifically which is accumulated in hepatic microsomes as a result of hypometabolism or dysbolism to thereby bring about the recovery of flexibility of a microsome lipid membrane, thus enhancing the enzymatic activity, and to significantly reduce a triglyceride, thus being prospective as an ameliorant for hepatic functions in treating fatty liver, hepatic cirrhosis, etc. The invention also provides a drug for hepatitis, which can inhibit the induction of hepatitis which is one of the factor causative of chronic hepatitis, hepatocirrhosis, hepatocellular carcinoma, hepatic insufficiency, etc.

摘要翻译： PCT No.PCT / JP92 / 01486 Sec。 371日期：1993年11月8日 102（e）日期1993年11月8日PCT提交1992年11月13日PCT公布。 公开号WO93 / 09772 日期：1993年5月27日。一种肝脏疾病药物，特别是肝功能改善剂和含有二十二碳六烯酸或其衍生物（DHA）作为活性成分的肝炎药物。 作为活性成分的DHA虽然也参与了在体内形成的胆固醇代谢并渗入血浆中，但主要代谢胆固醇特异性地代谢由于代谢异常或排尿困难导致的肝微粒体中累积的胆固醇，从而导致恢复 微粒体脂质膜的柔韧性，从而增强酶活性，并显着降低甘油三酯，因此作为治疗脂肪肝，肝硬化等肝功能的改善剂是预期的。本发明还提供了一种用于肝炎的药物，其中 可以抑制肝炎的诱导，这是导致慢性肝炎，肝硬化，肝细胞癌，肝功能不全等的因素之一。

公开(公告)号：US5246841A

公开(公告)日：1993-09-21

申请号：US781659

申请日：1991-10-25

申请人： Kazunaga Yazawa ,  Keiko Araki ,  Noriko Okazaki ,  Naganori Numao ,  Kiyosi Kondo

发明人： Kazunaga Yazawa ,  Keiko Araki ,  Noriko Okazaki ,  Naganori Numao ,  Kiyosi Kondo

IPC分类号： C12P7/64

CPC分类号： C12P7/6427 ,  C12P7/6472 ,  Y10S435/822 ,  Y10S435/876

摘要： A process for the production of eicosapentaenoic acid comprising the steps of a) culturing a microorganism capable of producing lipid containing eicosapentaenoic acid belonging to a genus selected from the group of Pseudomonas, Alteromonas and Shewanella, to produce lipid containing eicosapentaenoic acid, and b) recovering the eicosapentaenoic acid from the cultured product; a process for production of a lipid containing eicosapentaenoic acid comprising the steps of a) culturing a microorganism capable of producing lipid containing eicosapentaenoic acid belonging to a genus selected from the group of Pseudomonas, Alteromonas and Shewanella, to produce lipid containing eicosapentaenoic acid, and b) recovering the lipid containing eicosapentaenoic acid; and microorganisms capable of producing eicosapentaenoic acid belonging to a genus selected from the group consisting of Pseudomonas, Alteromonas, and Shewanella.

摘要翻译： 一种生产二十碳五烯酸的方法，包括以下步骤：a）培养能够产生属于选自假单胞菌属，异黄酮和希瓦氏菌属的属于二十碳五烯酸的脂质的微生物，以产生含有二十碳五烯酸的脂质，和b）回收 来自培养产物的二十碳五烯酸; 一种生产含有二十碳五烯酸的脂质的方法，包括以下步骤：a）培养能够产生属于选自假单胞菌属，异黄酮和希瓦氏菌属的属于二十碳五烯酸的脂质的微生物，以产生含有二十碳五烯酸的脂质，b ）回收含有二十碳五烯酸的脂质; 以及能够产生属于选自假单胞菌属，变形单子菌属和希瓦氏菌属的属的二十碳五烯酸的微生物。

公开(公告)号：US5189189A

公开(公告)日：1993-02-23

申请号：US689622

申请日：1991-04-23

申请人： Yoshihisa Misawa ,  Hisao Kondo ,  Tetsuya Tsutsumi ,  Masahiro Hayashi ,  Daisuke Sugimori ,  Yorishige Matsuba ,  Masashi Isozaki ,  Hiroharu Nishigaki ,  Kazunaga Yazawa ,  Kiyosi Kondo

发明人： Yoshihisa Misawa ,  Hisao Kondo ,  Tetsuya Tsutsumi ,  Masahiro Hayashi ,  Daisuke Sugimori ,  Yorishige Matsuba ,  Masashi Isozaki ,  Hiroharu Nishigaki ,  Kazunaga Yazawa ,  Kiyosi Kondo

IPC分类号： C07C51/487 ,  C07C67/60

CPC分类号： C07C51/487 ,  C07C67/60

摘要： [Object] A fatty acid-related polyunsaturated compound can be selectively purified using an industrially operable successive method under moderate conditions. [Content] A highly purified fatty acid-related polyunsaturated compound is obtained by a process comprising steps of forming a complex with silver on account of the high degree of unsaturation of the fatty acid-related polyunsaturated compound, isolating the complex from other fats on account of the hydrophilicity of the complex and then applying dissociating procedures to the complex to obtain the fatty acid-related polyunsaturated compound as a target substance on account of the hydrophobicity of the fatty acid-related polyunsaturated compound. As to the procedures for dissociation, addition of a dissociating agent and reduction of silver are disclosed. As to the dissociating agent, various compounds which produce insoluble composition with silver other then water are disclosed. Furthermore, an apparatus appropriately used to carry out this method is also disclosed.

公开(公告)号：US07993887B2

公开(公告)日：2011-08-09

申请号：US11920856

申请日：2006-04-11

申请人： Masaaki Nishihara ,  Masazumi Kamata ,  Kohji Yamaguchi ,  Kazunaga Yazawa

发明人： Masaaki Nishihara ,  Masazumi Kamata ,  Kohji Yamaguchi ,  Kazunaga Yazawa

IPC分类号： C12N1/20 ,  C12P7/64 ,  C12N9/20

CPC分类号： C12N9/20 ,  A23J7/00 ,  A23L33/135 ,  C12P7/6481 ,  C12R1/38 ,  C12Y301/01032

摘要： The present invention provides a new supply source of phospholipase A1, which is useful for phospholipid modification and lipid biochemical research, and offers a method capable of efficiently manufacturing a large amount of high DHA content phospholipids and lysophospholipids. A novel microorganism HFKI-0020 (FERM AP-20545) of the genus Pseudomonas which produces enzymes with phospholipase A1 activity can be used as a new supply source of enzymes with the phospholipase A1 activity. A lipid-modifying agent containing an effective dose of enzymes with the phospholipase A1 activity produced by those novel microorganisms allows the efficient mass-production of lysophospholipids.

摘要翻译： 本发明提供磷脂酶A1的新供应源，其可用于磷脂修饰和脂质生物化学研究，并提供能够有效地制造大量高DHA含量磷脂和溶血磷脂的方法。 可以使用产生具有磷脂酶A1活性的酶的假单胞菌属的新型微生物HFKI-0020（FERM AP-20545）作为具有磷脂酶A1活性的酶的新供应源。 含有有效剂量的由这些新型微生物产生的磷脂酶A1活性的酶的脂质改性剂允许有效量产生溶血磷脂。

公开(公告)号：US5683898A

公开(公告)日：1997-11-04

申请号：US375709

申请日：1995-01-20

申请人： Kazunaga Yazawa ,  Akiko Yamada ,  Seishi Kato ,  Kiyosi Kondo

发明人： Kazunaga Yazawa ,  Akiko Yamada ,  Seishi Kato ,  Kiyosi Kondo

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12P7/64 ,  C07H19/00 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/93 ,  C12P7/6427 ,  C12P7/6472

摘要： There is provided an advantageous process for production of EPA by a gene recombinant technique wherein genes coding for biosynthesis enzymes for eicosapentaenoic acid (EPA) useful as pharmaceuticals, agrochemicals, foods, feeds or the like is obtained from microorganisms. EPA is produced by obtaining genes coding for eicosapentaenoic acid (EPA) biosynthesis enzymes, constructing a plasmid by joining the genes to a vector, transforming E. coli with the plasmid, and culturing the transformed E. coli.

摘要翻译： 提供了通过基因重组技术生产EPA的有利方法，其中可从微生物获得编码二十碳五烯酸（EPA）的生物合成酶的基因，其用作药物，农业化学品，食品，饲料等。 EPA通过获得编码二十碳五烯酸（EPA）生物合成酶的基因产生，通过将基因连接到载体构建质粒，用质粒转化大肠杆菌，并培养转化的大肠杆菌。

公开(公告)号：US4812441A

公开(公告)日：1989-03-14

申请号：US827500

申请日：1986-02-07

申请人： Yasuo Kawai ,  Kazunaga Yazawa

发明人： Yasuo Kawai ,  Kazunaga Yazawa

IPC分类号： C07K14/195 ,  A61K35/74 ,  A61K38/00 ,  A61P3/06 ,  A61P9/10 ,  C07K14/005 ,  C07K14/315 ,  C07K14/41 ,  C12P21/00 ,  C12R1/44 ,  C12R1/46 ,  A61K37/02

CPC分类号： C12R1/46 ,  C07K14/315 ,  A61K38/00 ,  Y10S435/885 ,  Y10S514/824 ,  Y10S530/825

摘要： A hypocholesterolemically active protein capable of reducing the blood cholesterol in mammals having the following characteristics:(a) Molecular weight by gel filtration: 30,000.+-.7,000(b) Isoelectric point: 7.9.+-.0.2(c) Amino acid composition (mole %):______________________________________ Glycine 25.23 Alanine 10.98 Glutamate 10.34 Asparaginate 8.20 Lysine 6.39 Leucine 5.58 Valine 5.41 Isoleucine 5.18 Threonine 4.23 Tyrosine 4.19 Serine 3.46 Proline 2.62 Arginine 2.60 Phenylalanine 2.51 Methionine 1.56 Histidine 1.30 Cysteine 0.16 ______________________________________ (d) Pattern of electrophoresis: a sharp band on the anode side on polyacrylamide gel electrophoresis. This hypocholesterolemically active protein is derived from a microorganism belonging to the genus Streptococcus.

摘要翻译： 具有以下特征的能够降低哺乳动物血液胆固醇的降胆固醇活性蛋白质：（a）凝胶过滤分子量：30,000 +/- 7000（b）等电点：7.9 +/- 0.2（c）氨基酸组成 ％）： - 甘氨酸25.23丙氨酸10.98-谷氨酸盐10.34天冬酰胺酸盐8.20 -Lysine 6.39亮氨酸5.58-碱性5.41异亮氨酸5.18-苏氨酸4.23酪氨酸4.19-丝氨酸3.46脯氨酸2.62-精氨酸2.60苯丙氨酸2.51--乙硫醇1.56组氨酸1.30-半胱氨酸0.16 - （d） 电泳图案：在聚丙烯酰胺凝胶电泳上阳极侧的尖带。 该低胆固醇活性蛋白质来自属于链球菌属的微生物。

公开(公告)号：US09290781B2

公开(公告)日：2016-03-22

申请号：US13824518

申请日：2011-09-26

申请人： Kazunaga Yazawa ,  Tomoyuki Susa ,  Shoji Gotoh ,  Yasuhito Tashiro ,  Junichi Kawashima ,  Shigeyuki Imamura

发明人： Kazunaga Yazawa ,  Tomoyuki Susa ,  Shoji Gotoh ,  Yasuhito Tashiro ,  Junichi Kawashima ,  Shigeyuki Imamura

IPC分类号： C12P13/06 ,  A61K31/685 ,  C12P7/64

CPC分类号： C12P13/06 ,  A61K31/685 ,  C12P7/6481

摘要： The present invention provides a method for producing a composition containing 2-acyl-lysophosphatidylserine, including (a) a step of obtaining a composition containing phosphatidylserine by allowing phospholipase D to act on a raw material containing phosphatidylcholine in the presence of serine and (b) a step of obtaining a composition containing 2-acyl-lysophosphatidylserine by allowing phospholipase A1 to act on the composition containing phosphatidylserine in the presence of one or more additives selected from the group consisting of the following (I), (II), and (III), in which (I) one or more salts selected from the group consisting of sulfate salts, phosphate salts, nitrate salts, citrate salts, and tartarate salts of monovalent cations, (II) a gum, and (III) an emulsifier.

摘要翻译： 本发明提供一种含有2-酰基 - 溶血磷脂酰丝氨酸的组合物的制造方法，其包括（a）在丝氨酸存在下使磷脂酶D作用于含有磷脂酰胆碱的原料而获得含有磷脂酰丝氨酸的组合物的工序，（b） 在一种或多种选自以下（I），（II）和（III）中的添加剂的存在下，通过使磷脂酶A1作用于含有磷脂酰丝氨酸的组合物，获得含有2-酰基 - 溶血磷脂酰丝氨酸的组合物的步骤 ），其中（I）选自一价阳离子的硫酸盐，磷酸盐，硝酸盐，柠檬酸盐和酒石酸盐中的一种或多种盐，（II）树胶和（III）乳化剂。