公开(公告)号：US4395487A

公开(公告)日：1983-07-26

申请号：US256194

申请日：1981-04-21

申请人： Narimasa Saito ,  Tatsuo Horiuchi

发明人： Narimasa Saito ,  Tatsuo Horiuchi

IPC分类号： C12Q1/40

CPC分类号： C12Q1/40

摘要： A method for the assay of .alpha.-amylase activity, which comprises adding an .alpha.-amylase-containing sample to maltohexaitol or maltohexaonic acid used as substrate, reacting, at the same time or subsequent to the addition, .alpha.-glucosidase with the resulting mixture, and determining the reaction product to assay the .alpha.-amylase activity.

摘要翻译： 一种测定α-淀粉酶活性的方法，其包括将含有α-淀粉酶的样品添加到用作底物的麦芽六糖醇或麦芽六糖酸中，同时或之后加入α-葡糖苷酶与所得混合物， 并测定反应产物以测定α-淀粉酶活性。

公开(公告)号：US4332897A

公开(公告)日：1982-06-01

申请号：US895602

申请日：1978-04-12

申请人： Eiichi Nakano ,  Narimasa Saito ,  Danji Fukushima

发明人： Eiichi Nakano ,  Narimasa Saito ,  Danji Fukushima

IPC分类号： C12N15/09 ,  C12N7/00 ,  C12N15/00 ,  C12R1/91

CPC分类号： C12N15/00

摘要： A novel bacteriophage whose DNA molecule has endonuclease-sensitivity only in the DNA region carrying genetic information for the production of phage coat proteins can be obtained by isolating an endonuclease-resistant mutant from one of the lambdoid bacteriophages and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins.

摘要翻译： 一种新的噬菌体，其DNA分子仅在携带用于产生噬菌体外壳蛋白的遗传信息的DNA区域中具有核酸内切酶敏感性，可以通过从其中一个羊瘟噬菌体中分离内切核酸酶抗性突变体并将所得噬菌体与羊瘟噬菌体 在携带用于产生外壳蛋白的遗传信息的DNA区域中具有核酸内切酶敏感性。

公开(公告)号：US4039384A

公开(公告)日：1977-08-02

申请号：US669757

申请日：1976-03-24

申请人： Masaru Suzuki ,  Narimasa Saito

发明人： Masaru Suzuki ,  Narimasa Saito

IPC分类号： C12N9/78 ,  C12N9/86 ,  C12Q1/34 ,  C12D13/10 ,  C07G7/02 ,  C12K1/00

CPC分类号： C12Q1/34 ,  C12N9/78 ,  C12N9/86 ,  G01N2333/978 ,  G01N2333/986 ,  Y10S435/815 ,  Y10S435/843 ,  Y10S435/85 ,  Y10S435/859

摘要： Creatinine amidohydrolase and creatine amidinohydrolase can be obtained by culturing a strain belonging to Genus Flavobacterium, Genus Micrococcus or Genus Corynebacterium and having an ability to produce creatinine amidohydrolase and creatine amidinohydrolase in the presence of creatinine, creatine or a mixture thereof and separating creatinine amidohydrolase and creatine amidinohydrolase from the cultured fluid.

摘要翻译： 肌酸酰氨基水解酶和肌酸酰氨基水解酶可以通过培养属于黄杆菌属，微球菌属或棒状杆菌属的菌株，并且在肌酸酐，肌酸或其混合物存在下具有产生肌酸酐酰胺水解酶和肌酸脒基水解酶的能力并分离肌酸酐酰胺水解酶和肌酸 来自培养液的脒基水解酶。

公开(公告)号：US4348478A

公开(公告)日：1982-09-07

申请号：US87167

申请日：1979-10-22

申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12R1/91

CPC分类号： C12N15/00

摘要： A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.

摘要翻译： 一种制备新的重组DNA的方法，其包括（1）用核酸内切酶切割温和的噬菌体DNA，所述温带噬菌体DNA具有参与噬菌体DNA复制的DNA片段中不具有内切核酸酶敏感区域，并将噬菌体DNA整合到 宿主染色体，但至少在DNA片段中携带外壳蛋白生产的遗传信息和携带预期遗传信息的另一个DNA，（2）向裂解DNA的混合物中加入DNA连接酶，和（3）从混合物中回收噬菌体 通过用携带预期信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段，缺失其外壳蛋白产生能力的DNA。

公开(公告)号：US4348477A

公开(公告)日：1982-09-07

申请号：US87166

申请日：1979-10-22

申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12R1/91

CPC分类号： C12N15/00

摘要： A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.

摘要翻译： 一种制备新型重组DNA的方法，其包括（1）用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA，其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中，但在其他DNA （2）将由所述切割产生的所有片段混合在一起，（3）将所述切割产生的所有片段混合在一起，其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA， 向混合物中加入DNA连接酶，（4）从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA，通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。