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公开(公告)号:US06190898B1
公开(公告)日:2001-02-20
申请号:US08609362
申请日:1996-03-01
IPC分类号: C12N914
CPC分类号: C12N9/2437 , C12Y302/01004
摘要: A method for preparing a crystalline cellulase enzyme is provided which comprises preparing an aqueous solution containing cellulase enzyme and adding to the aqueous solution a salt comprising an anion selected from the group consisting of sulfate, phosphate, formate, acetate, sorbate, chloride, bromide, fluoride or iodide, and a cation selected from the group consisting of sodium, ammonium, magnesium, potassium or calcium, wherein the aqueous solution is at a temperature between 10° C. and 60° C.
摘要翻译: 提供了一种制备结晶纤维素酶的方法,其包括制备含有纤维素酶的水溶液,并向该水溶液中加入包含选自硫酸盐,磷酸盐,甲酸盐,乙酸盐,山梨酸盐,氯化物,溴化物, 氟化物或碘化物,以及选自钠,铵,镁,钾或钙的阳离子,其中所述水溶液的温度为10℃至60℃。
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公开(公告)号:US06431370B1
公开(公告)日:2002-08-13
申请号:US09590371
申请日:2000-06-08
IPC分类号: C11D742
CPC分类号: C11D3/046 , C11D3/386 , C11D3/38672 , C11D11/0082 , C12N9/00 , C12N9/54 , C12N9/98 , C12P1/00 , Y10S435/816
摘要: Detergent formulations are prepared by directly agglomerating a fermentation broth extract, containing a detergent-type enzyme and a nonionic detergent-type surfactant, with a suitable detergent base mixture, without need for prior isolation of the enzyme.
摘要翻译: 通过将含有洗涤剂型酶和非离子洗涤剂型表面活性剂的发酵肉汤提取物与合适的洗涤剂基础混合物直接附聚而制备洗涤剂制剂,而不需要事先分离酶。
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公开(公告)号:US06105786A
公开(公告)日:2000-08-22
申请号:US887494
申请日:1997-07-02
CPC分类号: C11D3/046 , C11D11/0082 , C11D3/386 , C12N9/00 , C12N9/54 , C12P1/00 , Y10S435/816
摘要: A process is disclosed for preparing a detergent powder containing enzymes where the enzymes are extracted from a fermentation broth with a salt and surfactant mixture and directly agglomerated with detergent paste and dried to form the detergent powder. The process results in a two phase system where the enzyme is extracted into the surfactant rich phase and the second phase is salt rich. The process is especially useful for whole or clarified fermentation broths.
摘要翻译: 公开了一种制备含有酶的洗涤剂粉末的方法,其中用盐和表面活性剂混合物从发酵液中提取酶,并用洗涤剂糊剂直接附聚并干燥以形成洗涤剂粉末。 该过程产生两相体系,其中酶被萃取到富表面活性剂相中,第二相是富含盐的。 该方法对于整个或澄清的发酵液特别有用。
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公开(公告)号:US06423517B1
公开(公告)日:2002-07-23
申请号:US09215086
申请日:1998-12-18
IPC分类号: C12N998
摘要: Granules are prepared containing an admixture of protein and salt layered over an inert particle. A preferred amount of salt is about between 63.7 and 84.3% of the total weight of the admixture. Proteins include pharmaceutically important proteins such as hormones, or industrially important proteins such as enzymes including proteases, amylases, lipases and cellulases capable of hydrolyzing substrates such as stains. Inert particles include inorganic salts, sugars, sugar alcohols, small organic molecules such as organic acids or salts, and minerals such as clays or silicates. A binder such as starch or polyethylene oxide may be mixed in with the admixture. A barrier material such as an inorganic salt or organic acid or salt may be in the admixture or coated over the admixture layer. A coating layer of a soluble or water dispersible film-forming polymer may be between the inert particle and admixture layer and/or over the admixture layer. The granules may also contain plasticizers, extenders, lubricants, pigments and anti-agglomeration agents. A preferred method for preparing the granules is by spraying a solution or slurry of the admixture onto the inert particles while fluidized in a fluid-bed coater.
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公开(公告)号:US20120214727A1
公开(公告)日:2012-08-23
申请号:US13361860
申请日:2012-01-30
申请人: Nathaniel T. Becker , Robert I. Christensen, JR. , Alfred L. Gaertner , Mahmood M. Ghani , Douglas A. Dale
发明人: Nathaniel T. Becker , Robert I. Christensen, JR. , Alfred L. Gaertner , Mahmood M. Ghani , Douglas A. Dale
CPC分类号: C12N9/48 , C11D3/38672 , C11D11/0088
摘要: A granule having high stability and low dust is described. The granule includes a hydrated barrier material having moderate or high water activity. Also described are methods of producing the granules.
摘要翻译: 描述了具有高稳定性和低灰尘的颗粒。 颗粒包括具有中等或高水分活性的水合阻隔材料。 还描述了生产颗粒的方法。
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公开(公告)号:US20110300201A1
公开(公告)日:2011-12-08
申请号:US13132069
申请日:2009-11-03
IPC分类号: A01N25/28 , C12N9/42 , C12N9/26 , C12N9/24 , C12N9/18 , A01P1/00 , C12N9/04 , C12N9/20 , C12N9/02 , D06L3/11 , C12S11/00 , A01N37/18 , C12N9/48 , C12N9/08
CPC分类号: C11D17/0039 , C11D3/386 , C11D3/38672 , C11D3/38681
摘要: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.
摘要翻译: 本发明提供包括酶/底物共递送系统的方法,组合物,系统和试剂盒。 液体递送系统包括至少一种包封在水溶性聚合物基质中的酶和在其中聚合物基质不溶性的载体液体中的酶的底物。 当加入水时,聚合物基体溶解,酶从基质中释放出来,允许对基底进行催化作用。
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公开(公告)号:US20090120873A1
公开(公告)日:2009-05-14
申请号:US12209424
申请日:2008-09-12
申请人: Nathaniel T. Becker , Ryszard Braun , Robert I. Christensen, JR. , Stefan Ekbom , Robin Fong , Alexandra Schuler
发明人: Nathaniel T. Becker , Ryszard Braun , Robert I. Christensen, JR. , Stefan Ekbom , Robin Fong , Alexandra Schuler
CPC分类号: B01D65/00 , B01D61/14 , B01D63/10 , B01D65/02 , B01D65/08 , B01D2311/06 , B01D2313/19 , B01D2321/02 , B01D2321/04 , B01D2321/10 , B01D2321/12 , B01D2321/20 , B01D2321/2008 , B01D2321/2083 , B01D2311/14 , B01D2311/165
摘要: Filtration processes and systems are provided for the separation of a filterable fluid stream by a filtration membrane module with uniform transmembrane pressure and flux along the membrane and internal control of membrane fouling via intermittent periodic reduction of the pressure differential between the permeate and retentate sides of the membrane and/or backwashing cycles during separation, recovery, and/or purification of proteins, peptides, nucleic acids, biologically produced polymers and other compounds or materials from aqueous fluids.
摘要翻译: 提供了过滤方法和系统,用于通过过滤膜组件分离可过滤流体流,所述过滤膜组件具有沿着膜的均匀的跨膜压力和通量以及膜污染的内部控制,通过间歇性周期性地减少渗透物和渗余物侧之间的压力差 在从水性流体分离,回收和/或纯化蛋白质,肽,核酸,生物制备的聚合物和其它化合物或其它化合物的过程中,膜和/或反冲洗循环。
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公开(公告)号:US06461672B1
公开(公告)日:2002-10-08
申请号:US09640394
申请日:2000-08-16
申请人: Antti Kosola , Ryszard Braun , Nathaniel T. Becker , Robert L. Christensen, Jr. , Ville Kyläkoski , Alfred L. Gaertner
发明人: Antti Kosola , Ryszard Braun , Nathaniel T. Becker , Robert L. Christensen, Jr. , Ville Kyläkoski , Alfred L. Gaertner
IPC分类号: B05D700
摘要: A fluidized bed coating apparatus is provided and includes a fluid inlet adapted to be connected to a source of fluidizing fluid. The fluid inlet has an entrance opening having a first diameter and an exit opening downstream of the entrance opening. The exit opening has a second diameter that is larger than the first diameter. The inlet also includes an expanding inlet section, between the entrance opening and the exit opening, that has an average diameter that increases in a direction from said entrance opening to said exit opening. A fluid distribution plate is provided adjacent the exit opening and includes a plurality of through-passages to allow the even and distributed passage of a fluidizing fluid therethrough. A chamber having a chamber inlet and a chamber outlet is provided and the fluid distribution plate is positioned between the exit opening and the chamber inlet. The chamber includes at least a first diverging section having an average diameter that increases in the direction from the chamber inlet downstream toward the chamber outlet. The symmetrical design of the coater provides an evenly distributed flow of fluid that enables efficient and complete fluidization and coating of very large particle beds.
摘要翻译: 提供了一种流化床涂覆设备,并包括适于连接到流化流体源的流体入口。 流体入口具有入口,其具有第一直径和入口开口下游的出口。 出口具有大于第一直径的第二直径。 入口还包括在入口和出口之间的扩张入口部分,其具有在从所述入口到所述出口的方向上增加的平均直径。 流体分配板邻近出口设置并且包括多个通道以允许流化流体均匀分布通过其中。 提供具有室入口和室出口的室,并且流体分配板位于出口和室入口之间。 该腔室至少包括第一发散部分,该第一发散部分具有在从腔室入口下游朝向腔室出口的方向上增加的平均直径。 涂布机的对称设计提供均匀分布的流体流动,能够高效,完全地流化和涂覆非常大的颗粒床。
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公开(公告)号:US08834865B2
公开(公告)日:2014-09-16
申请号:US13132069
申请日:2009-11-03
CPC分类号: C11D17/0039 , C11D3/386 , C11D3/38672 , C11D3/38681
摘要: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.
摘要翻译: 本发明提供包括酶/底物共递送系统的方法,组合物,系统和试剂盒。 液体递送系统包括至少一种包封在水溶性聚合物基质中的酶和在其中聚合物基质不溶性的载体液体中的酶的底物。 当加入水时,聚合物基体溶解,酶从基质中释放出来,允许对基底进行催化作用。
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公开(公告)号:US20080206830A1
公开(公告)日:2008-08-28
申请号:US12113422
申请日:2008-05-01
申请人: Nathaniel T. Becker , Robert I. Christensen , Alfred L. Gaertner , Mahmood M. Ghani , Douglas A. Dale
发明人: Nathaniel T. Becker , Robert I. Christensen , Alfred L. Gaertner , Mahmood M. Ghani , Douglas A. Dale
IPC分类号: C12N9/00
CPC分类号: C12N9/48 , C11D3/38672 , C11D11/0088
摘要: A granule having high stability and low dust is described. The granule includes a hydrated barrier material having moderate or high water activity. Also described are methods of producing the granules.
摘要翻译: 描述了具有高稳定性和低灰尘的颗粒。 颗粒包括具有中等或高水分活性的水合阻隔材料。 还描述了生产颗粒的方法。
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