公开(公告)号：US20080180673A1

公开(公告)日：2008-07-31

申请号：US11701292

申请日：2007-01-31

申请人： Nicholas M. Sampas ,  Rene P. Helbing ,  Bo U. Curry ,  Julie E. Fouquet ,  Carol T. Schembri

发明人： Nicholas M. Sampas ,  Rene P. Helbing ,  Bo U. Curry ,  Julie E. Fouquet ,  Carol T. Schembri

IPC分类号： G01N21/75

CPC分类号： G01N21/648 ,  G01N21/7703 ,  G01N2021/7709 ,  G01N2021/7786

摘要： A test system includes an optical medium, a binding agent capable of capturing a target complex, and a light detector. The optical medium provides a light path, and the binding agent is positioned to hold the target complex in an evanescent field created by propagation of light along the light path. The complex interacts with the evanescent field and emits light that the detector positioned to detect. The optical medium and the detector can be included in an optical integrated circuit where detected light passes through the optical medium transverse to the direction of the light path.

摘要翻译： 测试系统包括光学介质，能够捕获目标复合物的粘合剂和光检测器。 光学介质提供光路，并且粘合剂被定位成将目标复合体保持在通过沿着光路的光的传播产生的消逝场中。 复合体与ev逝场相互作用并发射检测器定位以检测的光。 光学介质和检测器可以包括在光学集成电路中，其中检测到的光穿过光学介质横向于光路的方向。

公开(公告)号：US08570518B2

公开(公告)日：2013-10-29

申请号：US11701292

申请日：2007-01-31

申请人： Nicholas M. Sampas ,  Rene P. Helbing ,  Bo U. Curry ,  Julie E. Fouquet ,  Carol T. Schembri

发明人： Nicholas M. Sampas ,  Rene P. Helbing ,  Bo U. Curry ,  Julie E. Fouquet ,  Carol T. Schembri

IPC分类号： G01N21/00

CPC分类号： G01N21/648 ,  G01N21/7703 ,  G01N2021/7709 ,  G01N2021/7786

摘要： A test system includes an optical medium, a binding agent capable of capturing a target complex, and a light detector. The optical medium provides a light path, and the binding agent is positioned to hold the target complex in an evanescent field created by propagation of light along the light path. The complex interacts with the evanescent field and emits light that the detector positioned to detect. The optical medium and the detector can be included in an optical integrated circuit where detected light passes through the optical medium transverse to the direction of the light path.

摘要翻译： 测试系统包括光学介质，能够捕获目标复合物的粘合剂和光检测器。 光学介质提供光路，并且粘合剂被定位成将目标复合体保持在通过沿着光路的光的传播产生的消逝场中。 复合体与ev逝场相互作用并发射检测器定位以检测的光。 光学介质和检测器可以包括在光学集成电路中，其中检测到的光穿过光学介质横向于光路的方向。

公开(公告)号：US10001486B2

公开(公告)日：2018-06-19

申请号：US11500626

申请日：2006-08-08

申请人： Bo U. Curry ,  Rene P. Helbing

发明人： Bo U. Curry ,  Rene P. Helbing

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G06F19/00 ,  G01N33/58 ,  G01N33/558

CPC分类号： G01N33/582 ,  G01N33/558

摘要： A method to calibrate measurements of a test analyte in a test sample including measuring at least one test-light level responsive to reactions of at least one reagent group and at least one reactive test analyte in the test sample and measuring at least one control-light level responsive to reactions of at least one reagent group and at least one control analyte in a control sample. Each control analyte is a known amount of at least one reactive test analyte. The method further includes determining a presence of the reactive test analyte in the test sample based on the measured test-light levels and control-light levels. The reagent group and the reactive test analyte react by attaching to each other.

公开(公告)号：US20080038711A1

公开(公告)日：2008-02-14

申请号：US11500626

申请日：2006-08-08

申请人： Bo U. Curry ,  Rene P. Helbing

发明人： Bo U. Curry ,  Rene P. Helbing

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G06F19/00

CPC分类号： G01N33/582 ,  G01N33/558

摘要： A method to calibrate measurements of a test analyte in a test sample including measuring at least one test-light level responsive to reactions of at least one reagent group and at least one reactive test analyte in the test sample and measuring at least one control-light level responsive to reactions of at least one reagent group and at least one control analyte in a control sample. Each control analyte is a known amount of at least one reactive test analyte. The method further includes determining a presence of the reactive test analyte in the test sample based on the measured test-light levels and control-light levels. The reagent group and the reactive test analyte react by attaching to each other.

摘要翻译： 校准测试样品中测试分析物的测量的方法，包括测量响应于至少一种试剂组和至少一种反应性测试分析物在测试样品中的反应的至少一种测试光级，并测量至少一种对照光 响应于对照样品中至少一个试剂组和至少一个对照分析物的反应。 每个对照分析物是已知量的至少一种反应性测试分析物。 所述方法还包括基于测量的测试光水平和控制光水平来确定测试样品中反应性测试分析物的存在。 试剂组和反应性试验分析物通过彼此连接而反应。

公开(公告)号：US20110301854A1

公开(公告)日：2011-12-08

申请号：US13073766

申请日：2011-03-28

申请人： Bo U. Curry ,  Nicholas M. Sampas

发明人： Bo U. Curry ,  Nicholas M. Sampas

IPC分类号： G06F19/00 ,  C40B30/04

CPC分类号： C12Q1/683 ,  C12Q1/6827 ,  G16B20/00 ,  G16B40/00 ,  C12Q2537/16 ,  C12Q2537/165

摘要： A method of estimating the allele-specific copy number of a SNP in a test genome is provided. In certain embodiments, the method involves: a) calculating a plurality of probability distribution functions that fit a plurality of log ratios indicating which alleles of a plurality of single nucleotide polymorphisms (SNPs) are present in diploid regions of a test and reference genome; and b) estimating the allele-specific copy number of a SNP of the test genome using said plurality of probability distribution functions.

摘要翻译： 提供了一种估计测试基因组中SNP等位基因特异性拷贝数的方法。 在某些实施方案中，所述方法包括：a）计算多个拟合多个对数比的概率分布函数，所述多个对数比指示在测试和参考基因组的二倍体区域中存在多个单核苷酸多态性（SNP）的哪些等位基因; 以及b）使用所述多个概率分布函数估计所述测试基因组的SNP的等位基因特异性拷贝数。

公开(公告)号：US20090062138A1

公开(公告)日：2009-03-05

申请号：US11897613

申请日：2007-08-31

申请人： Bo U. Curry ,  Nicholas M. Sampas ,  Robert A. Ach

发明人： Bo U. Curry ,  Nicholas M. Sampas ,  Robert A. Ach

IPC分类号： C40B30/04 ,  C40B40/06

CPC分类号： C12Q1/6837 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2525/161 ,  C12Q2523/113

摘要： An array-based method for performing SNP analysis is provided. In certain embodiments, the method may comprise: a) contacting a labeled genomic sample with an array comprising a first SNP-detecting oligonucleotide and a second SNP-detecting oligonucleotide that differ from each other by a single nucleotide, under hybridization conditions that provide binding equilibrium; and b) evaluating a SNP of said labeled genomic sample by comparing: i. binding of the labeled genomic sample to the first SNP-detecting oligonucleotide and ii. binding of the labeled genomic sample to said second SNP-detecting oligonucleotide.

摘要翻译： 提供了一种用于执行SNP分析的基于阵列的方法。 在某些实施方案中，所述方法可以包括：a）在提供结合平衡的杂交条件下，使标记的基因组样品与包含第一SNP检测寡核苷酸和第二SNP检测寡核苷酸的阵列接触，所述第一SNP检测寡核苷酸与单个核苷酸彼此不同 ; 和b）通过比较来评估所述标记的基因组样品的SNP： 标记的基因组样品与第一SNP检测寡核苷酸的结合，和ii。 标记的基因组样品与所述第二SNP检测寡核苷酸的结合。

公开(公告)号：US08043867B2

公开(公告)日：2011-10-25

申请号：US12409871

申请日：2009-03-24

申请人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

发明人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

IPC分类号： G01N33/53

CPC分类号： G01N33/54386 ,  G01N21/17 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/8483 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/062 ,  G01N2201/067 ,  Y10S435/805 ,  Y10S435/823 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/823 ,  Y10T436/25125

摘要： An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.

摘要翻译： 测定试片包括流路，样品接收区，标签，包括感兴趣区域的检测区和至少一个位置标记。 所述至少一个位置标记相对于所述感兴趣区域对齐，使得所述至少一个位置标记的位置指示所述感兴趣区域的位置。 诊断测试系统包括从测试条的暴露区域获得光强度测量的读取器，以及数据分析器，其执行以下至少一个：（a）基于至少一个（a）识别从测试区域获得的光强度测量中的一个 测量从所述至少一个参考特征获得的，以及（b）基于从所述至少一个参考特征获得的至少一个测量值生成修改所述读取器的至少一个操作参数的控制信号。

公开(公告)号：US20090325813A1

公开(公告)日：2009-12-31

申请号：US12215302

申请日：2008-06-26

申请人： Hui Wang ,  Bo U. Curry

发明人： Hui Wang ,  Bo U. Curry

IPC分类号： C40B30/04 ,  C40B40/06

CPC分类号： C12Q1/6837 ,  C12Q2545/113 ,  C12Q2525/207

摘要： Aspects of the disclosure are generally directed to methods, probes, probe compositions and kits for detecting or quantifying target oligonucleotides. In some embodiments, there are provided methods for determining the level of target oligonucleotides, such as a small RNA (e.g., miRNA), in a sample. In some embodiments, the methods comprise analyzing hybridization of target oligonucleotides to a test microarray; analyzing hybridization of a known amount of reference oligonucleotides (having the same sequences as the target oligonucleotides) to a calibration microarray; and determining the level of the target oligonucleotides in the sample by comparing the hybridization of the target oligonucleotides with the hybridization of the reference oligonucleotides.

摘要翻译： 本公开的方面通常涉及用于检测或定量靶寡核苷酸的方法，探针，探针组合物和试剂盒。 在一些实施方案中，提供了用于确定样品中靶寡核苷酸水平的方法，例如小RNA（例如miRNA）。 在一些实施方案中，所述方法包括分析靶寡核苷酸与测试微阵列的杂交; 分析已知量的参考寡核苷酸（具有与靶寡核苷酸相同的序列）与校准微阵列的杂交; 并通过比较靶寡核苷酸的杂交与参考寡核苷酸的杂交来确定样品中靶寡核苷酸的水平。

公开(公告)号：US20090180926A1

公开(公告)日：2009-07-16

申请号：US12409871

申请日：2009-03-24

申请人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

发明人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

IPC分类号： G01N31/22

CPC分类号： G01N33/54386 ,  G01N21/17 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/8483 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/062 ,  G01N2201/067 ,  Y10S435/805 ,  Y10S435/823 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/823 ,  Y10T436/25125

摘要： An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.

公开(公告)号：US06870166B2

公开(公告)日：2005-03-22

申请号：US10087619

申请日：2002-02-28

申请人： Bo U. Curry ,  Andreas N. Dorsel ,  Kyle J. Schleifer ,  Debra A. Sillman

发明人： Bo U. Curry ,  Andreas N. Dorsel ,  Kyle J. Schleifer ,  Debra A. Sillman

IPC分类号： G01N21/64 ,  G01J1/20

CPC分类号： G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6484

摘要： A maximum sensitivity optical scanning system is disclosed. It finds use in a variety of applications, including the reading of biopolymeric arrays. It operates by scanning sample at a setting selected to result in signal saturation for some, but not all available data. Subsequent scans of the same area are taken at lower sensitivity settings (in terms of detector gain and/or excitation light source gain or attenuation) and data from at least the previously saturated regions is obtained. If system sensitivity is set too low to produce useful results, optional features may adjust sensitivity upward and follow with an increased sensitivity scan as a remedial measure. Full signal sensitivity is better preserved as most needed in taking data for the weakest signals first with the high-level scan. Data for sample producing stronger signals that can better tolerate photobleaching is then taken in one way or another.

摘要翻译： 公开了一种最大灵敏度的光学扫描系统。 它可用于各种应用，包括读取生物聚合物阵列。 它通过在选定的设置上扫描样品来产生一些信号饱和而不是所有可用数据进行操作。 在较低的灵敏度设置（在检测器增益和/或激发光源增益或衰减方面）进行相同区域的后续扫描，并且获得来自至少先前饱和区域的数据。 如果系统灵敏度设置太低而无法产生有用的结果，可选功能可以向上调整灵敏度，并采用增加的灵敏度扫描作为补救措施。 完全的信号灵敏度更好地保留，因为首先采用高级扫描为最弱信号采集数据最为需要。 然后以一种或另一种方式获取产生更好的耐光漂白信号的样品的数据。