公开(公告)号：US20070105883A1

公开(公告)日：2007-05-10

申请号：US10551417

申请日：2004-03-29

申请人： Nobuaki Kushida ,  Naoko Watanabe ,  Takashi Yaguchi ,  Fumikazu Yokoyama ,  Goh Tsujiuchi ,  Takako Okuda

发明人： Nobuaki Kushida ,  Naoko Watanabe ,  Takashi Yaguchi ,  Fumikazu Yokoyama ,  Goh Tsujiuchi ,  Takako Okuda

IPC分类号： A61K31/4747 ,  C07D471/10

CPC分类号： C07D471/20 ,  C12P17/182 ,  C12R1/80

摘要： This invention provides novel PF1270A substance, PF1270B substance and PF1270C substance represented by the following formula (1) or pharmaceutically acceptable salts thereof, a method for producing the same and a pharmaceutical composition which comprises at least one of the same as the active ingredient. Since a group of the PF1270 substances of the invention show high affinity for histamine H3 receptor, they are expected as novel histamine H3 receptor ligands useful as medicaments.

摘要翻译： 本发明提供由下式（1）表示的新型PF1270A物质，PF1270B物质和PF1270C物质或其药学上可接受的盐，其制备方法和含有至少一种作为活性成分的药物组合物。 由于本发明的一组PF1270物质对组胺H 3 H 3受体表现出高亲和力，所以它们被认为是用作药物的新型组胺H 3 N 3受体配体。

公开(公告)号：US07501431B2

公开(公告)日：2009-03-10

申请号：US10551417

申请日：2004-03-29

申请人： Nobuaki Kushida ,  Naoko Watanabe ,  Takashi Yaguchi ,  Fumikazu Yokoyama ,  Goh Tsujiuchi ,  Takako Okuda

发明人： Nobuaki Kushida ,  Naoko Watanabe ,  Takashi Yaguchi ,  Fumikazu Yokoyama ,  Goh Tsujiuchi ,  Takako Okuda

IPC分类号： A61K31/4375 ,  C07D471/20

CPC分类号： C07D471/20 ,  C12P17/182 ,  C12R1/80

摘要： This invention provides novel PF1270A substance, PF1270B substance and PF1270C substance represented by the following formula (1) or pharmaceutically acceptable salts thereof, a method for producing the same and a pharmaceutical composition which comprises at least one of the same as the active ingredient. Since a group of the PF1270 substances of the invention show high affinity for histamine H3 receptor, they are expected as novel histamine H3 receptor ligands useful as medicaments.

摘要翻译： 本发明提供由下式（1）表示的新型PF1270A物质，PF1270B物质和PF1270C物质或其药学上可接受的盐，其制备方法和含有至少一种作为活性成分的药物组合物。 由于本发明的一组PF1270物质对组胺H3受体表现出高亲和力，所以它们被期望作为可用作药物的新型组胺H3受体配体。

公开(公告)号：US10053679B2

公开(公告)日：2018-08-21

申请号：US13638538

申请日：2010-03-31

申请人： Fumikazu Yokoyama

发明人： Fumikazu Yokoyama

IPC分类号： C12N9/24 ,  C12N9/42 ,  C12P19/02 ,  C12P19/14 ,  D06M16/00 ,  D21C5/00 ,  D21C5/02 ,  D21H21/10 ,  A23K20/189 ,  A23K10/14

CPC分类号： C12N9/2437 ,  A23K10/14 ,  A23K20/189 ,  C12N9/2445 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01021 ,  D06M16/003 ,  D21C5/005 ,  D21C5/025 ,  D21H21/10 ,  Y02E50/16 ,  Y02W30/648

摘要： An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and β-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and β-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.

公开(公告)号：US08975057B2

公开(公告)日：2015-03-10

申请号：US13391598

申请日：2010-08-17

申请人： Fumikazu Yokoyama ,  Kengo Yokoyama ,  Nobuko Mazuka

发明人： Fumikazu Yokoyama ,  Kengo Yokoyama ,  Nobuko Mazuka

IPC分类号： C12N15/56 ,  C12P19/14 ,  A23K1/165 ,  D21C5/00 ,  D21H17/00 ,  C12N9/42 ,  D21H17/22 ,  D21H21/10

CPC分类号： C12N9/2445 ,  A23K10/14 ,  A23K10/32 ,  C12P19/14 ,  C12Y302/01021 ,  D06M15/15 ,  D21C5/005 ,  D21H17/005 ,  D21H17/22 ,  D21H21/10

摘要： By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic β-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified β-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high β-glucosidase activity.

摘要翻译： 通过疏水层析和强碱性阴离子交换色谱法的组合，成功鉴定了解淀粉菌（Acremonium cellulolyticus）的新颖高度疏水性的葡萄糖苷酶。 此外，分离与鉴定的β-葡糖苷酶相对应的基因。 当基因的碱基序列引入多个修饰时，该基因在高水平的绿色木霉中成功表达，并且表达产物成功表现出高的葡萄糖苷酶活性。

公开(公告)号：US20130023014A1

公开(公告)日：2013-01-24

申请号：US13638538

申请日：2010-03-31

申请人： Fumikazu Yokoyama

发明人： Fumikazu Yokoyama

IPC分类号： C12N9/42 ,  C12N15/80 ,  C12N1/19 ,  A23K1/165 ,  C12P19/14 ,  C12S11/00 ,  C12S99/00 ,  D21C9/00 ,  C12N15/56 ,  C12N1/15

CPC分类号： C12N9/2437 ,  A23K10/14 ,  A23K20/189 ,  C12N9/2445 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01021 ,  D06M16/003 ,  D21C5/005 ,  D21C5/025 ,  D21H21/10 ,  Y02E50/16 ,  Y02W30/648

摘要： An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and β-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and β-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.

摘要翻译： 目的是通过分离含有纤维素分解酶的纤维素酶基因（含有葡聚糖酶或葡萄糖苷酶）并从其核苷酸序列测序来鉴定内切葡聚糖酶和葡萄糖苷酶基因。 本发明人将已知内切葡聚糖酶和葡萄糖苷酶的氨基酸序列彼此进行了深入比较，以发现解纤维解淀粉中的氨基酸序列的保守区域，并根据该信息设计了各种引物。 使用如此设计的各种引物进行PCR，并将基因组DNA或cDNA作为模板进行。 结果获得了内切葡聚糖酶和葡萄糖苷酶的基因片段。 基于基因片段设计引物，进行PCR扩增9个内切葡聚糖酶和葡萄糖苷酶的基因。 对其核苷酸序列进行测序，完成本发明。

公开(公告)号：US20120148706A1

公开(公告)日：2012-06-14

申请号：US13391598

申请日：2010-08-17

申请人： Fumikazu Yokoyama ,  Kengo Yokoyama ,  Nobuko Mazuka

发明人： Fumikazu Yokoyama ,  Kengo Yokoyama ,  Nobuko Mazuka

IPC分类号： C12N15/63 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  A23K1/165 ,  C12P19/14 ,  C12S11/00 ,  C12S9/00 ,  C12S3/08 ,  C11D3/386 ,  C12N15/56 ,  C12N9/42

CPC分类号： C12N9/2445 ,  A23K10/14 ,  A23K10/32 ,  C12P19/14 ,  C12Y302/01021 ,  D06M15/15 ,  D21C5/005 ,  D21H17/005 ,  D21H17/22 ,  D21H21/10

摘要： By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic β-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified β-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high β-glucosidase activity.

摘要翻译： 通过疏水层析和强碱性阴离子交换色谱法的组合，成功鉴定了解淀粉菌（Acremonium cellulolyticus）的新颖高度疏水性的葡萄糖苷酶。 此外，分离与鉴定的β-葡糖苷酶相对应的基因。 当基因的碱基序列引入多个修饰时，该基因在高水平的绿色木霉中成功表达，并且表达产物成功表现出高的葡萄糖苷酶活性。