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32 条记录 (耗时 0.026 秒)

    Amino acid sequence of L-phenylalanine ammonia-lyase
    1.
    发明授权
    Amino acid sequence of L-phenylalanine ammonia-lyase 失效
    L-苯丙氨酸氨裂解酶的氨基酸序列

    公开(公告)号：US06472196B1

    公开(公告)日：2002-10-29

    申请号：US08214018

    申请日：1994-03-15

    申请人： Nobuhiro Fukuhara ,  Setsuo Yoshino ,  Kaoru Yamamoto ,  Tomoyuki Se ,  Satori Sone ,  Yoshiyuki Nakajima ,  Maki Suzuki ,  Nobuyoshi Makiguchi

    发明人： Nobuhiro Fukuhara ,  Setsuo Yoshino ,  Kaoru Yamamoto ,  Tomoyuki Se ,  Satori Sone ,  Yoshiyuki Nakajima ,  Maki Suzuki ,  Nobuyoshi Makiguchi

    IPC分类号： C12N988

    CPC分类号： C12P13/222 ,  C12N9/88

    摘要： In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in Rhodosporidium toruloides, and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3′-terminus of the promoter region and the 5′-terminus of the terminator region. Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established. Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.

    摘要翻译： 为了提供利用遗传工程技术生产L-苯丙氨酸氨裂解酶（PAL）所必需的技术信息，已经在Rhodosporidium toruloides中阐明了PAL的结构基因和PAL的氨基酸序列，新的重组DNA质粒 （例如，pSW101，pYtrp6和pKY201）是通过将编码PAL基因的DNA链插入到启动子区域的3'端和终止区域的5'末端而产生的。此外，具有这样一种新颖的转化体 已经建立了重组DNA质粒，并且通过生长这样一种新的转化体以使培养中产生和积累PAL来生产PAL的方法已被建立。此外，已经建立了一种生产新技术 在通过上述新方法制备的PAL存在下，使氨供体与肉桂酸反应，生成L-苯丙氨酸。

    Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign product thereby
    4.
    发明授权
    Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign product thereby 失效
    通过控制培养基中的糖浓度和由此制造异物的方法来调节外源基因的表达的方法

    公开(公告)号：US5139935A

    公开(公告)日：1992-08-18

    申请号：US659472

    申请日：1991-02-25

    申请人： Nobuhiro Fukuhara ,  Setsuo Yoshino ,  Satori Sone ,  Yoshiyuki Nakajima ,  Nobuyoshi Makiguchi

    发明人： Nobuhiro Fukuhara ,  Setsuo Yoshino ,  Satori Sone ,  Yoshiyuki Nakajima ,  Nobuyoshi Makiguchi

    IPC分类号： C12N1/21 ,  C12N9/88 ,  C12N15/60 ,  C12N15/70

    CPC分类号： C12N15/70 ,  C12N9/88

    摘要： Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process so as to supress the suppression of the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

    摘要翻译： 携带通过将期望的外源基因插入到表达载体中以便允许其中表达所需外源基因的杂交质粒的大肠杆菌在含有作为碳源的大肠杆菌可利用的糖成分的培养基中培养 浓度为0.3％以上，能够抑制所希望的外源基因的表达。 将该大肠杆菌（即转化体）在第一种方法中将糖浓度保持在0.3％以上的培养基中培养，以抑制外源基因的抑制并支持足够的细胞生长，此后在少于 在第二过程中超过0.3％以释放表达的抑制，以便有效地产生外源基因产物，这导致外源基因产物在最终培养物中的高浓度。

    Antitheft apparatus for a vehicle
    6.
    发明授权
    Antitheft apparatus for a vehicle 失效
    车辆防盗装置

    公开(公告)号：US5949151A

    公开(公告)日：1999-09-07

    申请号：US932432

    申请日：1997-09-17

    申请人： Yoshiyuki Nakajima

    发明人： Yoshiyuki Nakajima

    IPC分类号： B60R25/01 ,  B60R25/045 ,  B60R25/10 ,  B60R25/104 ,  B60R25/20 ,  B60R25/30 ,  F02D17/04 ,  F02D29/02 ,  F02D45/00 ,  F02P11/04 ,  B60R25/04

    CPC分类号： B60R25/04 ,  B60R25/1004

    摘要： An electronic control member for preventing the stealing of a vehicle and an electronic control member for controlling ignition timing of an ignition device formed integrally and disposed in one package to thereby provide an antitheft apparatus for a vehicle which is small, economical, and highly capable of preventing theft. The antitheft apparatus for a vehicle includes an immobilizer, an antitheft electronic control member, an ignition device, a sensor, a remote control member and an alarm member.

    摘要翻译： 一种用于防止车辆盗窃的电子控制构件和用于控制一体形成并设置在一个封装中的点火装置的点火正时的电子控制构件，从而提供一种小型，经济和高能力的用于车辆的防盗装置 防盗。 用于车辆的防盗装置包括防盗器，防盗电子控制构件，点火装置，传感器，遥控构件和报警构件。

    Driving mechanism for a type drum in a printer
    7.
    发明授权
    Driving mechanism for a type drum in a printer 失效
    打印机类型滚筒的驱动机构

    公开(公告)号：US4602561A

    公开(公告)日：1986-07-29

    申请号：US704426

    申请日：1985-02-22

    申请人： Yoshiyuki Nakajima

    发明人： Yoshiyuki Nakajima

    IPC分类号： B41J7/36 ,  B41J1/48 ,  B41J7/34 ,  B41J23/04 ,  B41J9/10

    CPC分类号： B41J7/34 ,  B41J1/48 ,  B41J23/04 ,  Y10T74/19633

    摘要： An impact printer has a type drum, a plurality of hammers, a type drum gear secured to a shaft of the type drum, and a gear train including a drive gear and an intermediate gear engaged with the drive gear and type drum gear for driving the type drum. A lever is provided to rotate about a shaft of the drive gear. The intermediate gear is rotatably supported on the lever at one end thereof, and a spring is provided for urging the lever in the direction that the intermediate gear engages with the type drum gear. The rotational direction of the type drum gear is such that the rotation of the type drum gear causes the intermediate gear to revolve around the drive gear to brake the type drum.

    摘要翻译： 冲击式打印机具有类型鼓，多个锤，固定到型鼓的轴的类型鼓齿轮和包括驱动齿轮和与驱动齿轮啮合的中间齿轮的齿轮系和用于驱动 型鼓。 提供杠杆以围绕驱动齿轮的轴旋转。 中间齿轮在其一端可旋转地支撑在杠杆上，并且设置弹簧以沿中间齿轮与型鼓齿轮接合的方向推动杆。 类型鼓齿轮的旋转方向使得类型鼓齿轮的旋转使得中间齿轮围绕驱动齿轮旋转以制动型滚筒。

    Photoelectron Measuring Device
    8.
    发明申请
    Photoelectron Measuring Device 审中-公开
    光电子测量装置

    公开(公告)号：US20070205363A1

    公开(公告)日：2007-09-06

    申请号：US10592529

    申请日：2005-03-11

    申请人： Yoshiyuki Nakajima ,  Daisuke Yamashita

    发明人： Yoshiyuki Nakajima ,  Daisuke Yamashita

    IPC分类号： G01N23/227

    CPC分类号： G01N23/227

    摘要： To provide a photoelectron measuring device that enables handling of samples in the atmosphere. A photoelectron measuring device that emits ultraviolet light from an ultraviolet light generating source 1 onto a sample S while changing the wavelength and detects the wavelength at which photoelectrons start to be discharged from the sample S, with the section up to an emission outlet 2 being housed in a non-oxygen environment and a sample stand 4 being disposed so that the distance from the emission outlet 2 to the sample S is 7 mm or less.

    摘要翻译： 提供能够处理大气中的样品的光电子测量装置。 一种光电子测量装置，其在改变波长的同时将紫外光发生源1的紫外光发射到样品S上，并且检测从样品S开始放出光电子的波长，直到发射出口2被容纳 在非氧环境中，并且样品台4设置成使得从排出口2到样品S的距离为7mm以下。

    Image forming apparatus
    10.
    发明授权
    Image forming apparatus 失效
    图像形成装置

    公开(公告)号：US06804473B2

    公开(公告)日：2004-10-12

    申请号：US10447158

    申请日：2003-05-29

    申请人： Tomokazu Nakamura ,  Masaaki Inoo ,  Shinsuke Ubayashi ,  Yusuke Obuchi ,  Yoshiyuki Nakajima ,  Atsuteru Oikawa ,  Hitoshi Kato

    发明人： Tomokazu Nakamura ,  Masaaki Inoo ,  Shinsuke Ubayashi ,  Yusuke Obuchi ,  Yoshiyuki Nakajima ,  Atsuteru Oikawa ,  Hitoshi Kato

    IPC分类号： G03G1500

    CPC分类号： H04N1/00015 ,  B65H29/62 ,  B65H2301/4213 ,  B65H2701/176 ,  B65H2801/09 ,  B65H2801/15 ,  G03G15/6538 ,  H04N1/00002 ,  H04N1/00029 ,  H04N1/0005 ,  H04N1/00068 ,  H04N1/00079 ,  H04N1/00082 ,  H04N1/00092 ,  H04N1/00633

    摘要： An image forming apparatus has an abnormality detecting device for detecting the abnormality of the sheet, an abnormal sheet containing portion for containing therein the sheet of which the abnormality has been detected, a normal sheet containing portion for temporarily containing therein a normal sheet of which the abnormality has not been detected, and a controlling portion for controlling the image forming portion so that the abnormal sheet is contained in the abnormal sheet containing portion, and that an image formed on the abnormal sheet may be formed on a predetermined subsequent sheet subsequent to the abnormal sheet. The normal sheet between the abnormal sheet and the predetermined subsequent sheet is contained in the normal sheet containing portion after the abnormal sheet is contained in the abnormal sheet containing portion, and the normal sheet contained in the normal sheet containing portion is delivered after the predetermined subsequent sheet has been delivered.

    摘要翻译： 图像形成装置具有用于检测纸张的异常的异常检测装置，用于在其中容纳检测到异常的纸张的异常纸张容纳部分，用于暂时容纳其中正常纸张的正常纸张容纳部分， 还没有检测到异常，以及用于控制图像形成部分的控制部分，使得异常片材被包含在异常片材容纳部分中，并且形成在异常片材上的图像可以形成在预定的随后的片材之后 异常表。 在异常片材包含在异常片材容纳部分中之后，正常片材容纳部分中包含异常片材和预定后续片材之间的正常片材，并且在预定的随后的部分之后传送包含在普通片材容纳部分中的标准片材 工作表已交付。