公开(公告)号：US20170307440A1

公开(公告)日：2017-10-26

申请号：US15514084

申请日：2015-09-25

Applicant: Northwestern University

Inventor： Ben Urban ,  Hao F. Zhang ,  Cheng Sun ,  Biqin Dong

IPC: G01J1/42 ,  G01N33/483 ,  G01N21/84

Abstract: Certain examples disclose systems and methods for imaging a target. An example method includes: a) activating a subset of light-emitting molecules in a wide field area of a target using an excitation light; b) capturing one or more images of the light emitted from the subset of the molecules illuminated with the excitation light; c) localizing one or more activated light emitting molecules using one or more single molecule microscopic methods to obtain localization information; d) simultaneously capturing spectral information for the same localized activated light emitting molecules using one or more spectroscopic methods; e) resolving one or more non-diffraction limited images of the area of the target using a combination of the localization and spectral information for the localized activated light emitting molecules; and 0 displaying the one or more non-diffraction limited images.

公开(公告)号：US20190025476A1

公开(公告)日：2019-01-24

申请号：US16068819

申请日：2017-01-09

Applicant: Northwestern University

Inventor： Cheng Sun ,  Hao F. Zhang ,  Biqin Dong ,  Wenzhong Liu ,  Kieren J. Patel

IPC: G02B5/00 ,  G02B19/00

Abstract: Systems and methods to generate spatially coherent electromagnetic radiation are disclosed. An example method includes receiving two or more incident wavelengths of electromagnetic radiation; applying the two or more incident wavelengths of electromagnetic radiation to an array of features; generating two or more spatially coherent optical resonating modes through the interaction of the one or more incident wavelengths of electromagnetic radiation and the array of features; and coupling the two or more spatially coherent optical resonating modes to two or more spatially coherent propagating wavelengths of electromagnetic radiation, wherein the spatially coherent propagating wavelengths of electromagnetic radiation are identical to the two or more incident wavelengths of electromagnetic radiation. An example system includes an array of features configured to receive wavelengths of electromagnetic radiation; medium(s) configured to generate spatially coherent optical resonating mode(s); and medium(s) configured to generate spatially coherent propagating wavelength(s) of electromagnetic radiation.

公开(公告)号：US20180088048A1

公开(公告)日：2018-03-29

申请号：US15584018

申请日：2017-05-01

Applicant: Northwestern University

Inventor： Biqin Dong ,  Janel L. Davis ,  Cheng Sun ,  Hao F. Zhang ,  Kieren J. Patel ,  Ben Urban ,  Vadim Backman ,  Luay Almassalha ,  Yolanda Stypula-Cyrus ,  The-Quyen Nguyen

IPC: G01N21/64 ,  C12Q1/6825 ,  G01N33/53

CPC classification number: G01N21/6428 ,  C12Q1/6825 ,  C12Q1/6841 ,  G01N21/6458 ,  G01N33/5302 ,  G01N2021/6441

Abstract: The devices, methods, and systems of the present disclosure provide for spectroscopic super-resolution microscopic imaging. In some examples, spectroscopic super-resolution microscopic imaging may be referred to or comprise spectroscopic photon localization microscopy (SPLM), a method which may employ the use of extrinsic labels or tags in a test sample suitable for imaging. In some examples spectroscopic super-resolution microscopic or spectroscopic photon localization microscopy (SPLM) may not employ extrinsic labels and be performed using the intrinsic contrast of the test sample or test sample material.Generally, spectroscopic super-resolution microscopic imaging may comprise resolving one or more non-diffraction limited images of an area of a test sample by acquiring both localization information of a subset of molecules using microscopic methods known in the art, and simultaneously or substantially simultaneously, acquiring spectral data about the same or corresponding molecules in the subset. This method maybe useful to detect a variety of features in cellular material for the molecular characterization of cells and disease.

公开(公告)号：US10830639B2

公开(公告)日：2020-11-10

申请号：US15514084

申请日：2015-09-25

Applicant: Northwestern University

Inventor： Ben Urban ,  Hao F. Zhang ,  Cheng Sun ,  Biqin Dong

IPC: G01N21/64 ,  G01J1/42 ,  G02B21/36 ,  G01N21/65 ,  G02B21/00 ,  G02B27/58 ,  G01N21/84 ,  G01N33/483

Abstract: Certain examples disclose systems and methods for imaging a target. An example method includes: a) activating a subset of light-emitting molecules in a wide field area of a target using an excitation light; b) capturing one or more images of the light emitted from the subset of the molecules illuminated with the excitation light; c) localizing one or more activated light emitting molecules using one or more single molecule microscopic methods to obtain localization information; d) simultaneously capturing spectral information for the same localized activated light emitting molecules using one or more spectroscopic methods; e) resolving one or more non-diffraction limited images of the area of the target using a combination of the localization and spectral information for the localized activated light emitting molecules; and f) displaying the one or more non-diffraction limited images.

公开(公告)号：US10524664B2

公开(公告)日：2020-01-07

申请号：US15583615

申请日：2017-05-01

Applicant: Northwestern University

Inventor： Wenzhong Liu ,  Lian Duan ,  Hao F. Zhang ,  Kieren J. Patel ,  Hao Li ,  Biqin Dong ,  Amani A. Fawzi

IPC: G06T7/00 ,  A61B5/00 ,  A61B5/145

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine a flow rate of fluid in the target; angiography is also performed using one or more fast scanning methods to determine a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

公开(公告)号：US20180020922A1

公开(公告)日：2018-01-25

申请号：US15583615

申请日：2017-05-01

Applicant: Northwestern University

Inventor： Wenzhong Liu ,  Lian Duan ,  Hao F. Zhang ,  Kieren J. Patel ,  Hao Li ,  Biqin Dong ,  Amani A. Fawzi

IPC: A61B5/00 ,  G06T7/00

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine a flow rate of fluid in the target; angiography is also performed using one or more fast scanning methods to determine a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.