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公开(公告)号：US20230092310A1
公开(公告)日：2023-03-23
申请号：US17799323
申请日：2021-02-15
发明人： Michael Chun Hao Chen , Sihong Chen , Christopher Dulson , Sumit Kalsi , Gordon Ross McInroy , Stephanie Reikine
IPC分类号： B01L3/00
摘要： Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.
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公开(公告)号：US20220127588A1
公开(公告)日：2022-04-28
申请号：US17407509
申请日：2021-08-20
IPC分类号： C12N9/12
摘要： The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Polμ, Polβ, Polλ, and Polθ of any species or the homologous amino acid sequence of X family polymerases of any species and uses thereof.
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公开(公告)号：US20230372939A1
公开(公告)日：2023-11-23
申请号：US17918871
申请日：2021-04-14
发明人： Michael Chun Hao Chen , Sumit Kalsi , Laurence Livingstone Bell , Gordon Ross McInroy , David Zhitomirsky , Luke M Slominski , RicK J Paolini, JR. , Cristina Visani
IPC分类号： B01L3/00
CPC分类号： B01L3/502792 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/161 , B01L2400/0427
摘要： A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the conformal layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the matrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.
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公开(公告)号：US20230167477A1
公开(公告)日：2023-06-01
申请号：US17982080
申请日：2022-11-07
CPC分类号： C12P21/00 , B01L3/502761 , C12M21/18 , C12M41/32 , C12M47/10 , B01L2400/043 , B01L2400/0427 , B01L2200/04 , B01L2200/0652 , B01L2200/16
摘要： The invention provided herein relates to methods for protein synthesis, characterisation and purification on a digital microfluidic device.
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公开(公告)号：US20230151046A1
公开(公告)日：2023-05-18
申请号：US17916862
申请日：2021-04-06
摘要： The present invention relates to a compound according to Formula (1c) or (1d): wherein, R1; R2; R3 and R6 are defined herein, and their use in methods of nucleic acid synthesis.
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公开(公告)号：US20230175030A1
公开(公告)日：2023-06-08
申请号：US17610900
申请日：2020-05-14
摘要： Disclosed an improved process for synthesising a nucleic acid strand using cycles of template independent enzyme extension. Having one or more of the amino groups on the base heterocyclic groups masked with protecting groups helps to prevent secondary structure in the extended strand, thereby improving access of the enzyme to the 3′ OH terminus for extension.
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公开(公告)号：US20230057330A1
公开(公告)日：2023-02-23
申请号：US17965750
申请日：2022-10-13
发明人： Michael Chun Hao Chen , Sumit Kalsi , Laurence Livingstone Bell , Gordon Ross McInroy , David Zhitomirsky , Luke Slominski , Rick Paolini, JR. , Cristina Visani
IPC分类号： B01L3/00
摘要： A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.
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公开(公告)号：US20220259632A1
公开(公告)日：2022-08-18
申请号：US17436314
申请日：2020-03-09
申请人： Nuclera Nucleics Ltd
摘要： The invention relates to methods and kits for the synthesis of oligonucleotides via controlled, localised deprotection of 3′-ONH2 groups on a solid support.
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公开(公告)号：US20230117083A1
公开(公告)日：2023-04-20
申请号：US17794320
申请日：2021-01-22
发明人： Tobias William Barr Ost , Gordon Ross McInroy , Zachary Beyer Gaber , Harold Swerdlow , Cecilia Tognoloni
摘要： The invention relates to improved methods of enzymatic solid-supported nucleic acid synthesis that make use of terminal deoxynucleotidyl transferase (TdT) enzymes or modified terminal deoxynucleotidyl transferase (TdT) enzymes on polyacrylamide type supports. The invention further relates to the use of kits comprising said enzymes in a method of solid-supported nucleic acid synthesis.
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公开(公告)号：US20230042211A1
公开(公告)日：2023-02-09
申请号：US17969323
申请日：2022-10-19
发明人： Michael Chun Hao Chen , Sihong Chen , Christopher Dulson , Sumit Kalsi , Gordon Ross McInroy , Stephanie Reikine
摘要： Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.
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