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1.发明公开公开(公告)号：US20230323426A1
公开(公告)日：2023-10-12
申请号：US18074790
申请日：2022-12-05
IPC分类号： C12Q1/6806 , C12N15/10 , C12Q1/6869 , C12Q1/6874
CPC分类号： C12Q1/6806 , C12N15/1093 , C12N15/1065 , C12Q1/6869 , C12Q1/6874
摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.
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公开(公告)号：US20180355348A1
公开(公告)日：2018-12-13
申请号：US16000505
申请日：2018-06-05
IPC分类号： C12N15/10
CPC分类号： C12N15/1065 , C12N15/1093 , C12Q1/6806 , C12Q1/6874 , C12Q2523/125 , C40B50/18 , C12Q2523/101 , C12Q2525/191 , C12Q2535/122 , C12Q2537/143 , C12Q2537/159 , C12Q2563/159 , C12Q2563/179
摘要： Provided herein are methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells. The present methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques to characterize the methylation profiles of large numbers of single cells quickly, accurately and inexpensively.
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3.发明申请公开(公告)号：US20180023119A1
公开(公告)日：2018-01-25
申请号：US15656947
申请日：2017-07-21
IPC分类号： C12Q1/68
摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.
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公开(公告)号：US11535883B2
公开(公告)日：2022-12-27
申请号：US15656947
申请日：2017-07-21
IPC分类号： C40B50/00 , C12Q1/6806 , C12Q1/6874 , C12N15/10 , C12Q1/6869
摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.
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公开(公告)号：US11999945B2
公开(公告)日：2024-06-04
申请号：US17305230
申请日：2021-07-01
申请人： Illumina, Inc.
CPC分类号： C12N15/1006 , B01L3/502761 , C12N15/1065 , C40B30/00 , C40B60/02 , C40B80/00
摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
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公开(公告)号：US11965158B2
公开(公告)日：2024-04-23
申请号：US17189916
申请日：2021-03-02
申请人： Illumina, Inc.
IPC分类号： C12N15/10 , C12N9/22 , C12Q1/6806
CPC分类号： C12N15/1093 , C12N9/22 , C12Q1/6806 , C12Q1/6806 , C12Q2521/507 , C12Q2565/514 , C12N15/1093 , C12Q2521/101 , C12Q2535/122 , C12Q2563/179
摘要： Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, sequencing the template library, and capturing the contiguity information.
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公开(公告)号：US20240018584A1
公开(公告)日：2024-01-18
申请号：US18359299
申请日：2023-07-26
申请人： ILLUMINA, INC.
IPC分类号： C12Q1/6874 , C12Q1/6858 , B01L3/00
CPC分类号： C12Q1/6874 , C12Q1/6858 , B01L3/502761 , C12Q1/6806
摘要： Embodiments of systems, methods, and compositions provided herein relate to on bead tagmentation and droplet indexing. Some embodiments include performing co-assays on partitioned beads, including nucleic acid sequencing, indexed PCR, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.
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公开(公告)号：US20210277388A1
公开(公告)日：2021-09-09
申请号：US17189916
申请日：2021-03-02
申请人： Illumina, Inc.
IPC分类号： C12N15/10 , C12Q1/6806 , C12N9/22
摘要： Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, sequencing the template library, and capturing the contiguity information.
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公开(公告)号：US11085036B2
公开(公告)日：2021-08-10
申请号：US16663060
申请日：2019-10-24
申请人： Illumina, Inc.
发明人： Steven Norberg , Dmitry Pokholok , Ramesh Ramji , Frank J. Steemers , Fan Zhang
摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
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公开(公告)号：US20210102194A1
公开(公告)日：2021-04-08
申请号：US16625100
申请日：2019-06-04
发明人： Frank J. Steemers , Jay Shendure , Junyue Cao
IPC分类号： C12N15/10
摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes nucleic acids that represent the whole transcriptomes from the plurality of single cells. In one embodiment, the nucleic acids include three index sequences. Also provided herein are compositions, such as compositions that include the nucleic acids having three index sequences.
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