公开(公告)号：US20080160598A1

公开(公告)日：2008-07-03

申请号：US11881428

申请日：2007-07-27

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： C12M1/00

CPC分类号： G01N21/76

摘要： The present invention provides an organic monolith reactor and the preparation method thereof, wherein the reactor is possible to manufacture even into a small size and even at room temperature, and the manufacturing process is simple and relatively short period of time is required.In an organic monolith reactor according to the present invention, a horseradish peroxidase is embedded onto a microscopic area with a photopolymerized polymer. The method of preparing an organic monolith reactor according to the present invention comprises (a) dispersing a horseradish peroxidase in a mixed solution of a photopolymerizable monomer and a photopolymerizing agent, (b) introducing the dispersed solution into a microscopic area, and (c) performing a photo-irradiation onto the microscopic area at room temperature.

摘要翻译： 本发明提供一种有机整料反应器及其制备方法，其中反应器甚至可以制造成小尺寸甚至室温，制造工艺简单，需要较短的时间。 在根据本发明的有机整料反应器中，将辣根过氧化物酶用光聚合物包埋在微观区域上。 根据本发明的制备有机整料反应器的方法包括（a）将辣根过氧化物酶分散在可光聚合单体和光聚合剂的混合溶液中，（b）将分散溶液引入微观区域，和（c） 在室温下在微观区域进行光照射。

公开(公告)号：US20090045059A1

公开(公告)日：2009-02-19

申请号：US12218320

申请日：2008-07-14

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： G01N27/44721

摘要： The present invention provides a method and apparatus for measuring the membrane potential of red blood cells using electrophoretic analysis that analyzes red blood cells whose membrane potentials are reversed due to red blood cell agglutination.The method includes: dipping an electrophoresis cell into an electrophoresis tank that contains an electrophoresis solution such that a current can flow at the middle of the electrophoresis tank; introducing a specimen for measuring red blood cells to a zero point S (base point) at the center of the electrophoresis cell; capturing the image of the electrophoresis tank with time; performing RGB (red, green, and blue) analysis on image data of each of the captured images; measuring the moving coordinates of the brightness of each B component from the base point for each pixel, thereby analyzing the images; and measuring variation in the electrophoresis direction indicated by the accumulation result of the moving coordinates of the analysis data toward a cathode and an anode. The apparatus includes: an electrophoresis device A that includes an electrophoresis tank 1a having three tanks 101a, 10a, and 101a and an electrophoresis cell 3a dipped into the central tank 10a; an image capturing device 6a that captures the image of the electrophoresis cell 3a with time; and an analyzing device 7a that analyzes image data of the captured image. The electrophoresis solution 2a is contained in the three tanks such that it can flow through the three tanks, and a current can flow through the electrophoresis solution 2a.

摘要翻译： 本发明提供一种使用电泳分析来测量红细胞的膜电位的方法和装置，其分析由于红细胞凝集而膜电位反转的红细胞。 该方法包括：将电泳细胞浸入含有电泳溶液的电泳槽中，使电流可以在电泳槽的中间流动; 将用于测量红细胞的样本引入电泳池中心的零点S（基点）; 随时间捕捉电泳槽的图像; 对每个拍摄图像的图像数据执行RGB（红，绿和蓝）分析; 从每个像素的基点测量每个B分量的亮度的移动坐标，从而分析图像; 并且测量由分析数据向阴极和阳极移动的坐标的累积结果所指示的电泳方向的变化。 该装置包括：电泳装置A，其具有电泳槽1a，具有浸渍在中央箱10a中的三个槽101a，10a，101a和电泳槽3a; 拍摄电泳池3a的图像的图像拍摄装置6a; 以及分析摄像图像的图像数据的分析装置7a。 电泳液2a包含在三个罐中，使得其可以流过三个罐，并且电流可以流过电泳溶液2a。

公开(公告)号：US20080027222A1

公开(公告)日：2008-01-31

申请号：US11494991

申请日：2006-07-28

申请人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

IPC分类号： C07D473/04 ,  C07D207/444

CPC分类号： G01N33/582 ,  C07D207/444 ,  C07D473/34

摘要： Disclosed herein are novel chemiluminescent substances prepared by freezing or lyophilizing a solution of a peroxidized nitrogen-containing five-membered cyclic compound.The chemiluminescent substances have a sensitivity comparable to that of luminol chemiluminescence, are highly water soluble, have no incorporation of impurities, are not degraded during storage, have high reproducibility in measurement results, and are very suitable for use in the detection and quantification of various substances in many applications, e.g., clinical applications.

摘要翻译： 本文公开了通过将过氧化的含氮五元环化合物的溶液冷冻或冻干而制备的新型化学发光物质。 化学发光物质具有与鲁米诺化学发光相当的灵敏度，高度水溶性，不掺杂杂质，在储存过程中不降解，测量结果重现性高，非常适合用于检测和定量各种 许多应用中的物质，如临床应用。

公开(公告)号：US07288412B2

公开(公告)日：2007-10-30

申请号：US10903077

申请日：2004-07-30

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： G01N33/48

CPC分类号： G01N33/80 ,  G01N27/44786

摘要： A method for determining the distribution of glycation of erythrocytic membranes includes steps of bonding magnetic beads having a boric acid group with sugars on the surfaces of erythrocytic membranes, and moving the erythrocytes bonded with the boric acid-magnetic beads under an influence of an electromagnetic force. A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline. Subsequently, a specimen of erythrocytes, which are bonded with the boric acid-magnetic beads, is infused into the container through the filling hole.

摘要翻译： 确定红细胞膜糖基化分布的方法包括将具有硼酸基团的磁珠与红细胞膜表面的糖结合的步骤，以及在电磁力的影响下移动与硼酸 - 磁珠结合的红细胞 。 用于测定红细胞膜糖化分布的装置包括：作为电磁泳用细胞的容器，与直流电源连接的阴极射线和阳极射线，以及位于 容器 容器具有包含阴极孔，阳极孔，阴极孔和用于试样的阳极孔之间的填充孔的上表面和用于释放气泡的排泄孔。 将生理盐水倒入容器后，将阴极射线和阳极射线分别插入孔中以浸泡在盐水中。 随后，与硼酸 - 磁珠结合的红细胞样品通过填充孔注入容器。

公开(公告)号：US20050026241A1

公开(公告)日：2005-02-03

申请号：US10903077

申请日：2004-07-30

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： G01N33/483 ,  G01N27/00 ,  G01N27/447 ,  G01N33/53 ,  G01N33/80 ,  G01N1/30 ,  G01N33/48

CPC分类号： G01N33/80 ,  G01N27/44786

摘要： A method for determining the distribution of glycation of erythrocytic membranes includes steps of bonding magnetic beads having a boric acid group with sugars on the surfaces of erythrocytic membranes, and moving the erythrocytes bonded with the boric acid-magnetic beads under an influence of an electromagnetic force. A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline. Subsequently, a specimen of erythrocytes, which are bonded with the boric acid-magnetic beads, is infused into the container through the filling hole.

摘要翻译： 确定红细胞膜糖基化分布的方法包括将具有硼酸基团的磁珠与红细胞膜表面的糖结合的步骤，以及在电磁力的影响下移动与硼酸 - 磁性珠粒结合的红细胞 。 用于测定红细胞膜糖化分布的装置包括：作为电磁泳用细胞的容器，与直流电源连接的阴极射线和阳极射线，以及位于 容器 容器具有包含阴极孔，阳极孔，阴极孔和用于试样的阳极孔之间的填充孔的上表面和用于释放气泡的排泄孔。 将生理盐水倒入容器后，将阴极射线和阳极射线分别插入孔中以浸泡在盐水中。 随后，与硼酸 - 磁珠结合的红细胞样品通过填充孔注入容器。

公开(公告)号：US07977493B2

公开(公告)日：2011-07-12

申请号：US11494991

申请日：2006-07-28

申请人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

IPC分类号： C07D233/70

CPC分类号： G01N33/582 ,  C07D207/444 ,  C07D473/34

摘要： Chemiluminescent substances are prepared by freezing or lyophilizing a solution of a peroxidized nitrogen-containing five-membered cyclic compound.The chemiluminescent substances have a sensitivity comparable to that of luminol chemiluminescence, are highly water-soluble, have no incorporation of impurities, are not degraded during storage, have high reproducibility in measurement results, and are very suitable for use in the detection and quantification of various substances in many applications, e.g., clinical applications.

摘要翻译： 通过将过氧化的含氮五元环化合物的溶液冷冻或冻干来制备化学发光物质。 化学发光物质具有与鲁米诺化学发光相当的灵敏度，高度水溶性，不含杂质，储存期间不降解，测量结果重现性高，非常适合用于检测和定量 许多应用中的各种物质，如临床应用。

公开(公告)号：US20080026452A1

公开(公告)日：2008-01-31

申请号：US11862232

申请日：2007-09-27

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： C12M1/34

CPC分类号： G01N33/80 ,  G01N27/44786

摘要： A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline. Subsequently, a specimen of erythrocytes, which are bonded with the boric acid-magnetic beads, is infused into the container through the filling hole.

摘要翻译： 用于测定红细胞膜糖化分布的装置包括：作为电磁泳用细胞的容器，与直流电源连接的阴极射线和阳极射线，以及位于 容器 容器具有包含阴极孔，阳极孔，阴极孔和用于试样的阳极孔之间的填充孔的上表面和用于释放气泡的排泄孔。 将生理盐水倒入容器后，将阴极射线和阳极射线分别插入孔中以浸泡在盐水中。 随后，与硼酸 - 磁珠结合的红细胞样品通过填充孔注入容器。

公开(公告)号：US20070292890A1

公开(公告)日：2007-12-20

申请号：US11455474

申请日：2006-06-19

申请人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

IPC分类号： G01N33/53 ,  C12M3/00

CPC分类号： G01N21/76 ,  G01N21/05 ,  G01N21/6428 ,  G01N2021/0346

摘要： Disclosed herein are a chemiluminescence chip, which is used to detect and quantify various kinds of materials in the case of performing the enzyme labeling of a material to be measured and performing the chemiluminescence measurement of the activity of the labeled enzyme, and a chemiluminescence detecting apparatus using the same. The chemiluminescence chip is constructed such that a filling unit, in which an immobilized enzyme is filled, is mounted in a space having an inlet port, through which a material to be measured is introduced, and an outlet port, through which the material to be measured is discharged, on a supporting structure. The chemiluminescence detecting apparatus is constructed such that the above-described chemiluminescence chip is mounted in a dark box, a cooled CCD camera is mounted above the chemiluminescence chip, an inlet pipe, which is disposed outside the dark box, is connected to the inlet port of the filling unit of the chemiluminescence chip, and an outlet pipe, which is also disposed outside the dark box, is connected to the outlet port of the filling unit.

摘要翻译： 本发明公开了一种化学发光芯片，其用于在进行待测材料的酶标记和进行化学发光测量标记酶的活性的情况下检测和定量各种材料，以及化学发光检测装置 使用相同 化学发光芯片被构造成使得其中填充有固定化酶的填充单元安装在具有入口端口的空间中，待测量材料通过该入口和出口，通过该出口 测量放电，在支撑结构上。 化学发光检测装置的结构是将上述化学发光芯片安装在暗盒中，将冷却的CCD照相机安装在化学发光芯片的上方，设置在暗箱外部的入口管连接到入口 的化学发光芯片的填充单元，并且还设置在暗箱外部的出口管连接到填充单元的出口。

公开(公告)号：US20050250172A1

公开(公告)日：2005-11-10

申请号：US10649471

申请日：2003-08-27

申请人： Osamu Nozaki ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Hiroko Kawamoto

IPC分类号： C12M1/40 ,  C12Q1/28 ,  G01N21/59 ,  G01N21/76

CPC分类号： G01N21/76 ,  C12Q1/28

摘要： A method for determining hydrogen peroxide and a device for use in the method excellent at determining hydrogen peroxide in body fluid in the field of a clinical examination are provided. In the method for determining hydrogen peroxide, the intensity of light emitted by reaction of immobilized horseradish peroxidase, hydrogen peroxide and imidazoles in alkaline pH is measured. The device for determining hydrogen peroxide in body fluid includes a pump for chromatography, an autosampler, a mobile phase passage, having the pump and the autosampler for body fluid, a pump for chromatography, a mobile phase passage, having the pump for a solution of imidazoles and an alkaline buffer, a flow passage, a flow cell, packed with a horseradish peroxide immobilized stationary phase, a chemiluminometer, having the flow cell, a photomultiplier, provided on the chemiluminometer in contiguity with the surface of the flow cell for the chemiluminomter. In the device, the mobile phase passages and join into the flow passage, and the flow passage is connecting to the chemiluminometer.

摘要翻译： 提供了一种用于确定过氧化氢的方法和用于在临床检查领域中测定体液中的过氧化氢的优异方法中使用的装置。 在测定过氧化氢的方法中，测定碱性pH下由固定化辣根过氧化物酶，过氧化氢和咪唑反应发出的光强度。 用于测定体液中过氧化氢的装置包括用于色谱的泵，自动进样器，流动相通道，其具有用于体液的泵和自动进样器，用于色谱的泵，流动相通道，具有用于溶液的溶液的泵 咪唑和碱性缓冲液，流通道，流通池，填充有固定有辣根过氧化物的固定相，具有流动池的化学发光计，与化学发光器的流动池的表面相邻设置在化学发光计上的光电倍增管 。 在该装置中，流动相通道并连接到流动通道中，并且流动通道连接到化学发光计。