公开(公告)号：US20080160598A1

公开(公告)日：2008-07-03

申请号：US11881428

申请日：2007-07-27

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： C12M1/00

CPC分类号： G01N21/76

摘要： The present invention provides an organic monolith reactor and the preparation method thereof, wherein the reactor is possible to manufacture even into a small size and even at room temperature, and the manufacturing process is simple and relatively short period of time is required.In an organic monolith reactor according to the present invention, a horseradish peroxidase is embedded onto a microscopic area with a photopolymerized polymer. The method of preparing an organic monolith reactor according to the present invention comprises (a) dispersing a horseradish peroxidase in a mixed solution of a photopolymerizable monomer and a photopolymerizing agent, (b) introducing the dispersed solution into a microscopic area, and (c) performing a photo-irradiation onto the microscopic area at room temperature.

摘要翻译： 本发明提供一种有机整料反应器及其制备方法，其中反应器甚至可以制造成小尺寸甚至室温，制造工艺简单，需要较短的时间。 在根据本发明的有机整料反应器中，将辣根过氧化物酶用光聚合物包埋在微观区域上。 根据本发明的制备有机整料反应器的方法包括（a）将辣根过氧化物酶分散在可光聚合单体和光聚合剂的混合溶液中，（b）将分散溶液引入微观区域，和（c） 在室温下在微观区域进行光照射。

公开(公告)号：US20090045059A1

公开(公告)日：2009-02-19

申请号：US12218320

申请日：2008-07-14

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： G01N27/44721

摘要： The present invention provides a method and apparatus for measuring the membrane potential of red blood cells using electrophoretic analysis that analyzes red blood cells whose membrane potentials are reversed due to red blood cell agglutination.The method includes: dipping an electrophoresis cell into an electrophoresis tank that contains an electrophoresis solution such that a current can flow at the middle of the electrophoresis tank; introducing a specimen for measuring red blood cells to a zero point S (base point) at the center of the electrophoresis cell; capturing the image of the electrophoresis tank with time; performing RGB (red, green, and blue) analysis on image data of each of the captured images; measuring the moving coordinates of the brightness of each B component from the base point for each pixel, thereby analyzing the images; and measuring variation in the electrophoresis direction indicated by the accumulation result of the moving coordinates of the analysis data toward a cathode and an anode. The apparatus includes: an electrophoresis device A that includes an electrophoresis tank 1a having three tanks 101a, 10a, and 101a and an electrophoresis cell 3a dipped into the central tank 10a; an image capturing device 6a that captures the image of the electrophoresis cell 3a with time; and an analyzing device 7a that analyzes image data of the captured image. The electrophoresis solution 2a is contained in the three tanks such that it can flow through the three tanks, and a current can flow through the electrophoresis solution 2a.

摘要翻译： 本发明提供一种使用电泳分析来测量红细胞的膜电位的方法和装置，其分析由于红细胞凝集而膜电位反转的红细胞。 该方法包括：将电泳细胞浸入含有电泳溶液的电泳槽中，使电流可以在电泳槽的中间流动; 将用于测量红细胞的样本引入电泳池中心的零点S（基点）; 随时间捕捉电泳槽的图像; 对每个拍摄图像的图像数据执行RGB（红，绿和蓝）分析; 从每个像素的基点测量每个B分量的亮度的移动坐标，从而分析图像; 并且测量由分析数据向阴极和阳极移动的坐标的累积结果所指示的电泳方向的变化。 该装置包括：电泳装置A，其具有电泳槽1a，具有浸渍在中央箱10a中的三个槽101a，10a，101a和电泳槽3a; 拍摄电泳池3a的图像的图像拍摄装置6a; 以及分析摄像图像的图像数据的分析装置7a。 电泳液2a包含在三个罐中，使得其可以流过三个罐，并且电流可以流过电泳溶液2a。

公开(公告)号：US20080027222A1

公开(公告)日：2008-01-31

申请号：US11494991

申请日：2006-07-28

申请人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

IPC分类号： C07D473/04 ,  C07D207/444

CPC分类号： G01N33/582 ,  C07D207/444 ,  C07D473/34

摘要： Disclosed herein are novel chemiluminescent substances prepared by freezing or lyophilizing a solution of a peroxidized nitrogen-containing five-membered cyclic compound.The chemiluminescent substances have a sensitivity comparable to that of luminol chemiluminescence, are highly water soluble, have no incorporation of impurities, are not degraded during storage, have high reproducibility in measurement results, and are very suitable for use in the detection and quantification of various substances in many applications, e.g., clinical applications.

摘要翻译： 本文公开了通过将过氧化的含氮五元环化合物的溶液冷冻或冻干而制备的新型化学发光物质。 化学发光物质具有与鲁米诺化学发光相当的灵敏度，高度水溶性，不掺杂杂质，在储存过程中不降解，测量结果重现性高，非常适合用于检测和定量各种 许多应用中的物质，如临床应用。

公开(公告)号：US20040179956A1

公开(公告)日：2004-09-16

申请号：US10798015

申请日：2004-03-11

申请人： Takao TSUDA, Chemco Scientific Co.,Ltd. ,  Motonori MUNESUE

发明人： Takao Tsuda ,  Motonori Munesue

IPC分类号： F04B039/00

CPC分类号： F04B19/24 ,  G01N2030/326

摘要： The liquid pump has a pressure vessel with end-openings for hermetically holding liquid therein. The liquid flows from one of the end-openings to the other end-opening by utilizing a difference in a coefficient of thermal expansion of the pressure vessel and the liquid caused by heating or cooling the pressure vessel and the liquid together. The pressure vessel is a tube having an inner diameter of about 0.5 mm or less and a length of about 10 m or more.

摘要翻译： 液体泵具有压力容器，该压力容器具有用于在其中气密保持液体的端部开口。 利用由压力容器和液体加热或冷却而导致的压力容器和液体的热膨胀系数的差异，液体从一个端部开口流到另一个端部开口。 压力容器是具有约0.5mm或更小的内径和约10μm或更大的长度的管。

公开(公告)号：US20030209146A1

公开(公告)日：2003-11-13

申请号：US10454140

申请日：2003-06-04

申请人： Takao Tsuda ,  Sun Plastics Co., Ltd ,  Motonori Munesue

发明人： Takao Tsuda ,  Motonori Munesue

IPC分类号： B01D053/02

CPC分类号： B01D53/02 ,  B01D2253/10 ,  B01D2253/106 ,  B01D2253/204 ,  B01J20/0211 ,  B01J20/06 ,  B01J20/103 ,  B01J20/28011 ,  B01J20/28023 ,  B01J20/28028 ,  B01J20/3204 ,  B01J20/3223 ,  B01J20/3225 ,  B01J20/3236 ,  B01J20/3295 ,  B01J2220/64 ,  Y10S55/05

摘要： A material is provided for capturing a chemical substance comprising fibers which carry metal alkoxide particles on the surfaces thereof. The metal alkoxide particles are made from an oligosilica, an oligozirconia, an oligotitiania, or the like. The fibers may include inorganic fibers, regenerated fibers and/or synthetic fibers. The material for capturing a chemical substance is produced by a method comprising steps of subjecting the fibers to a surface treatment for facilitating adhesion of the metal alkoxide particles to the surfaces of the fibers, forming the metal alkoxide particles on the surfaces of the fibers, and fixing the metal alkoxide particles to the surfaces of the fibers. A chemical substance-capturing tube, capable of capturing a trace of a chemical substance in a liquid or a gas, can be produced by placing the fibers, i.e., the material for capturing a chemical substance, in a hollow tube.

摘要翻译： 提供了用于捕获包含在其表面上携带金属醇盐颗粒的纤维的化学物质的材料。 金属醇盐粒子由低聚硅氧烷，低氧氧化锆，寡ia子等制成。 纤维可以包括无机纤维，再生纤维和/或合成纤维。 用于捕获化学物质的材料通过以下方法制备，所述方法包括以下步骤：使纤维进行表面处理以促进金属醇盐颗粒粘附到纤维表面，在纤维的表面上形成金属醇盐颗粒，以及 将金属醇盐颗粒固定在纤维的表面上。 可以通过将纤维（即用于捕获化学物质的材料）放置在中空管中来制造能够捕获液体或气体中的化学物质痕迹的化学物质捕获管。

公开(公告)号：US20030189001A1

公开(公告)日：2003-10-09

申请号：US10119132

申请日：2002-04-08

申请人： Takao Tsuda; Chemco Scientific Co., Ltd. ,  Motonori Munesue

发明人： Takao Tsuda ,  Shinya Kitagawa ,  Motonori Munesue

IPC分类号： B01D015/08

CPC分类号： B01J20/286 ,  B01J20/28054 ,  B01J20/28097 ,  B01J20/282 ,  B01J2220/54 ,  B01J2220/84 ,  G01N30/52 ,  G01N30/6078 ,  G01N2030/525 ,  G01N2030/567

摘要： In an open capillary column, a stationary phase is formed on the inner wall of a capillary tube. This stationary phase comprises a number of pores on the surface of the capillary tube, and each of pore has an expanded hollow in the stationary phase. A manufacturing method of an open capillary column comprises the steps of giving alkaline treatment to an inner wall of an open capillary tube, applying one of oligo silica, oligo zirconia and oligo titania on the inner wall, filling the open capillary tube with alkaline solution to form one of a silica layer, a zirconia layer and titania layer, discharging the alkaline solution and heat-treating the capillary tube to form a base of a stationary phase. The open capillary column of the present invention comprises a stationary phase having a very large surface area. It is useful for a separation analysis field such as liquid chromatography and electrochromatography.

摘要翻译： 在开放的毛细管柱中，在毛细管的内壁上形成固定相。 该固定相包括毛细管表面上的多个孔，并且每个孔在固定相中具有膨胀的中空。 开放毛细管柱的制造方法包括以下步骤：对开放毛细管的内壁进行碱处理，在内壁上施加低氧二氧化硅，低氧二氧化锆和低氧二氧化钛之一，用碱性溶液填充开放的毛细管， 形成二氧化硅层，氧化锆层和二氧化钛层之一，排出碱溶液并热处理毛细管以形成固定相的碱。 本发明的开放毛细管柱包括具有非常大的表面积的固定相。 它对于分离分析领域如液相色谱和电色谱是有用的。

公开(公告)号：US07977493B2

公开(公告)日：2011-07-12

申请号：US11494991

申请日：2006-07-28

申请人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

IPC分类号： C07D233/70

CPC分类号： G01N33/582 ,  C07D207/444 ,  C07D473/34

摘要： Chemiluminescent substances are prepared by freezing or lyophilizing a solution of a peroxidized nitrogen-containing five-membered cyclic compound.The chemiluminescent substances have a sensitivity comparable to that of luminol chemiluminescence, are highly water-soluble, have no incorporation of impurities, are not degraded during storage, have high reproducibility in measurement results, and are very suitable for use in the detection and quantification of various substances in many applications, e.g., clinical applications.

摘要翻译： 通过将过氧化的含氮五元环化合物的溶液冷冻或冻干来制备化学发光物质。 化学发光物质具有与鲁米诺化学发光相当的灵敏度，高度水溶性，不含杂质，储存期间不降解，测量结果重现性高，非常适合用于检测和定量 许多应用中的各种物质，如临床应用。

公开(公告)号：US20080026452A1

公开(公告)日：2008-01-31

申请号：US11862232

申请日：2007-09-27

申请人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

发明人： Osamu Nozaki ,  Hiroko Kawamoto ,  Motonori Munesue

IPC分类号： C12M1/34

CPC分类号： G01N33/80 ,  G01N27/44786

摘要： A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline. Subsequently, a specimen of erythrocytes, which are bonded with the boric acid-magnetic beads, is infused into the container through the filling hole.

摘要翻译： 用于测定红细胞膜糖化分布的装置包括：作为电磁泳用细胞的容器，与直流电源连接的阴极射线和阳极射线，以及位于 容器 容器具有包含阴极孔，阳极孔，阴极孔和用于试样的阳极孔之间的填充孔的上表面和用于释放气泡的排泄孔。 将生理盐水倒入容器后，将阴极射线和阳极射线分别插入孔中以浸泡在盐水中。 随后，与硼酸 - 磁珠结合的红细胞样品通过填充孔注入容器。

公开(公告)号：US20070292890A1

公开(公告)日：2007-12-20

申请号：US11455474

申请日：2006-06-19

申请人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

发明人： Osamu Nozaki ,  Motonori Munesue ,  Hiroko Kawamoto

IPC分类号： G01N33/53 ,  C12M3/00

CPC分类号： G01N21/76 ,  G01N21/05 ,  G01N21/6428 ,  G01N2021/0346

摘要： Disclosed herein are a chemiluminescence chip, which is used to detect and quantify various kinds of materials in the case of performing the enzyme labeling of a material to be measured and performing the chemiluminescence measurement of the activity of the labeled enzyme, and a chemiluminescence detecting apparatus using the same. The chemiluminescence chip is constructed such that a filling unit, in which an immobilized enzyme is filled, is mounted in a space having an inlet port, through which a material to be measured is introduced, and an outlet port, through which the material to be measured is discharged, on a supporting structure. The chemiluminescence detecting apparatus is constructed such that the above-described chemiluminescence chip is mounted in a dark box, a cooled CCD camera is mounted above the chemiluminescence chip, an inlet pipe, which is disposed outside the dark box, is connected to the inlet port of the filling unit of the chemiluminescence chip, and an outlet pipe, which is also disposed outside the dark box, is connected to the outlet port of the filling unit.

摘要翻译： 本发明公开了一种化学发光芯片，其用于在进行待测材料的酶标记和进行化学发光测量标记酶的活性的情况下检测和定量各种材料，以及化学发光检测装置 使用相同 化学发光芯片被构造成使得其中填充有固定化酶的填充单元安装在具有入口端口的空间中，待测量材料通过该入口和出口，通过该出口 测量放电，在支撑结构上。 化学发光检测装置的结构是将上述化学发光芯片安装在暗盒中，将冷却的CCD照相机安装在化学发光芯片的上方，设置在暗箱外部的入口管连接到入口 的化学发光芯片的填充单元，并且还设置在暗箱外部的出口管连接到填充单元的出口。

公开(公告)号：US07252016B2

公开(公告)日：2007-08-07

申请号：US10994124

申请日：2004-11-19

申请人： Takao Tsuda ,  Eiji Iizuka ,  Motonori Munesue

发明人： Takao Tsuda ,  Eiji Iizuka ,  Motonori Munesue

IPC分类号： G01N35/10

CPC分类号： G01N30/20 ,  G01N30/74

摘要： An injector has a sample chamber for retaining a small amount of sample, wherein a position of the sample chamber is controlled by transmitting light through the sample chamber and detecting the transmitted light so as to recognize the position of the sample chamber, and it is possible to reduce detection error compared to the conventional injectors that indirectly control the position of the sample chamber by transmitting light through the light passage which is adjacent to the sample chamber. A method for injecting a sample comprising the steps of: providing an injector which has a sample chamber for retaining a sample with an open month; transmitting light through the sample chamber; detecting the transmitted light so as to recognize a position of the sample chamber; and controlling the position of the sample chamber by using the detection result to connect the open month of the sample chamber to an open month of a sample receiving member in order to inject the sample into the sample receiving member.

摘要翻译： 注射器具有用于保持少量样品的样品室，其中通过将光透过样品室并检测透射光以便识别样品室的位置来控制样品室的位置，并且可能 以便与通过将光透过与样品室相邻的光通道间接地控制样品室的位置的常规喷射器相比降低检测误差。 一种用于注射样品的方法，包括以下步骤：提供具有用于保持样品的开放月份的样品室的注射器; 透过样品室; 检测透射光以识别样品室的位置; 以及通过使用检测结果来控制样品室的位置，以将样品室的开放月份连接到样品接收构件的开放月份，以便将样品注入样品接收构件。