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公开(公告)号：US06656682B1
公开(公告)日：2003-12-02
申请号：US09599032
申请日：2000-06-20
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , B01J2219/00387 , B01J2219/00527 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00722 , C12Q2565/507 , C12Q2565/513
摘要： A nucleic acid-immobilized substrate which comprises a carrier comprising a base material and a compound having a carbodiimide group or an isocyanate group carried by the base material, and the same kind or different kinds of nucleic acids immobilized in the form of dots through the carbodiimide group or the isocyanate group at a plurality of sites on the carrier.
摘要翻译： 一种核酸固定基质，其包含载体，所述载体包含基材和由所述基材携带的具有碳二亚胺基或异氰酸酯基的化合物，以及通过所述碳二亚胺以点形式固定的相同种类或不同种类的核酸 基团或载体上的多个位点处的异氰酸酯基团。
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公开(公告)号：US5700935A
公开(公告)日：1997-12-23
申请号：US577374
申请日：1995-12-22
申请人： Soichiro Takenishi , Osamu Suzuki , Hirohiko Yokomizo , Tatsuo Ichihara , Gen Masuda , Namiko Nakajima , Kazuko Komiya
发明人： Soichiro Takenishi , Osamu Suzuki , Hirohiko Yokomizo , Tatsuo Ichihara , Gen Masuda , Namiko Nakajima , Kazuko Komiya
IPC分类号： G01N33/58 , C07D495/04 , G01N33/532
CPC分类号： C07D495/04 , G01N33/532
摘要： The present invention relates to a carbodiimide derivative represented by the following general formula: W.sub.1 --X--N.dbd.C.dbd.N--Y--W.sub.2 --Z wherein W.sub.1 is a straight chain, branched chain or cyclic saturated or unsaturated aliphatic hydrocarbon group, a substituted or unsubstituted aryl group, a heteroaryl group, tertiary amino group or a tertiary or quaternary ammonium group; --W.sub.2 --Z is a quaternary ammonium group; X and Y are each independently a single bond or an alkylene group; and Z is a biotin group represented by the following formula: ##STR1## wherein n is 0 or 1. The derivative is useful as a label reagent for introducing a biotin group into a nucleic acid or a protein.
摘要翻译： 本发明涉及由以下通式表示的碳二亚胺衍生物：W1-XN = C = NY-W2-Z其中W1是直链，支链或环状饱和或不饱和脂族烃基，取代或未取代的芳基， 杂芳基，叔氨基或叔或季铵基团; -W2-Z是季铵基; X和Y各自独立地为单键或亚烷基; 并且Z是由下式表示的生物素基团：其中n为0或1的（a）。该衍生物可用作将生物素基团导入核酸或蛋白质的标记试剂。
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公开(公告)号：US07105299B2
公开(公告)日：2006-09-12
申请号：US10317449
申请日：2002-12-12
申请人： Shogo Moriya , Tatsuo Ichihara , Osamu Suzuki , Akihisa Urano , Syuiti Abe
发明人： Shogo Moriya , Tatsuo Ichihara , Osamu Suzuki , Akihisa Urano , Syuiti Abe
CPC分类号： C12Q1/6888 , C12Q2600/156 , C12Q2600/172
摘要： A kit comprising an oligonucleotide-immobilized substrate obtained by immobilizing, on a substrate, one or more kinds of oligonucleotides that enable detection of a polymorphism in a nucleotide sequence of mitochondrial DNA control region of chum salmon, of which standard is represented by the nucleotide sequence of SEQ ID NO: 8, at a position selected from the 10th, 30th, 42nd, 57th, 70th, 96th, 108th, 154th, 194th, 231st, 242nd, 250th, 260th, 339th, 340th, 386th, 395th, 401st and 471st positions is used to detect the polymorphism based on hybridization of the oligonucleotides with a nucleic acid derived from specimen chum salmon and thereby determine a haplotype of the specimen chum salmon.
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公开(公告)号：US5908746A
公开(公告)日：1999-06-01
申请号：US660295
申请日：1996-06-07
申请人： Osamu Suzuki , Naokazu Sasaki , Tatsuo Ichihara , Sanae Okada
发明人： Osamu Suzuki , Naokazu Sasaki , Tatsuo Ichihara , Sanae Okada
IPC分类号： G01N33/543 , C12Q1/68 , C07H21/04 , C12Q1/00
CPC分类号： G01N33/54353
摘要： A method is provided, comprising the steps of reacting a biologically active first substance immobilized on a carrier with a second substance capable of specifically binding the first substance, and detecting a non-bound part of the second substance or a bound part of the second substance indirectly bound to the carrier through binding between the first and second substances so that the first substance or the second substance in a sample is analyzed, wherein the carrier carries a compound having 2 to 100 carbodiimide groups, and the first substance is immobilized on the carrier through the carbodiimide groups so that the active substance such as protein and nucleic acid is bound to the carrier conveniently, efficiently, and tightly.
摘要翻译： 提供了一种方法，包括以下步骤：将固定在载体上的生物活性第一物质与能够特异性结合第一物质的第二物质反应，并检测第二物质的未结合部分或第二物质的结合部分 通过第一和第二物质之间的结合间接地结合载体，从而分析样品中的第一物质或第二物质，其中载体携带具有2至100个碳二亚胺基团的化合物，并且第一物质固定在载体上 通过碳二亚胺基团，使得蛋白质和核酸等活性物质方便，有效，紧密地结合载体。
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公开(公告)号：US5789588A
公开(公告)日：1998-08-04
申请号：US931714
申请日：1997-09-16
申请人： Soichiro Takenishi , Osamu Suzuki , Hirohiko Yokomizo , Tatsuo Ichihara , Gen Masuda , Namiko Nakajima , Kazuko Komiya
发明人： Soichiro Takenishi , Osamu Suzuki , Hirohiko Yokomizo , Tatsuo Ichihara , Gen Masuda , Namiko Nakajima , Kazuko Komiya
IPC分类号： G01N33/58 , C07D495/04 , G01N33/532
CPC分类号： C07D495/04 , G01N33/532
摘要： The present invention relates to a carbodiimide derivative represented by the following general formula: W.sub.1 --X--N.dbd.C.dbd.N--Y--W.sub.2 --Z wherein W.sub.1 is a straight chain, branched chain or cyclic saturated or unsaturated aliphatic hydrocarbon group, a substituted or unsubstituted aryl group, a heteroaryl group, a tertiary amino group or a tertiary or quaternary ammonium group; --W.sub.2 --Z is a quaternary ammonium group; X and Y are each independently a single bond or an alkylene group; and Z is a biotin group represented by the following formula: ##STR1## wherein n is 0 or 1. The derivative is useful as a label reagent for introducing a biotin group into a nucleic acid or a protein.
摘要翻译： 本发明涉及由以下通式表示的碳二亚胺衍生物：W1-XN = C = NY-W2-Z其中W1是直链，支链或环状饱和或不饱和脂族烃基，取代或未取代的芳基， 杂芳基，叔氨基或叔或季铵基团; -W2-Z是季铵基; X和Y各自独立地为单键或亚烷基; 并且Z是由下式表示的生物素基团：其中n为0或1的（a）。该衍生物可用作将生物素基团导入核酸或蛋白质的标记试剂。
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公开(公告)号：US20080199968A1
公开(公告)日：2008-08-21
申请号：US11659660
申请日：2005-09-08
申请人： Tatsuo Ichihara , Mitsugu Usui
发明人： Tatsuo Ichihara , Mitsugu Usui
CPC分类号： G01N33/532 , C12N15/10 , C12Q1/682 , Y10T436/143333 , C12Q2537/149 , C12Q2525/161 , C12Q2525/155
摘要： To solve a problem occurring in the PALSAR method that a polymer would be formed in the state of unbound to a captured test gene and thus affect the quantitative characteristics as a nonspecific signal, it is intended to develop a technique whereby the polymer formation is controlled in the step of forming an assembly (polymer) of probes so that the polymer is formed exclusively on a test gene to thereby improve the sensitivity and quantitative characteristics. It is found that the polymer can be quantitatively formed and a nonspecific reaction can be inhibited by, in the step of forming a polymer by reacting plural kinds of probes having abilities to complementarily bind to each other, not adding or reacting these probes at once but starting with the reaction of a first probe in one group, and then reacting the second probe in the other group followed by the reactions of probes one by one (i.e., the first probe, the second probe, and so on).
摘要翻译： 为了解决在PALSAR方法中发生聚合物将以与捕获的测试基因无关的状态形成并因此影响作为非特异性信号的定量特征的问题，旨在开发一种技术，由此将聚合物形成控制在 形成探针的组件（聚合物）的步骤，使得聚合物专门在测试基因上形成，从而提高灵敏度和定量特性。 发现可以定量地形成聚合物，并且在通过使具有彼此互补结合能力的多种探针反应形成聚合物的步骤中不能一次添加或反应这些探针来抑制非特异性反应， 从一组中的第一探针的反应开始，然后使另一组中的第二探针反应，然后逐个进行探针的反应（即，第一探针，第二探针等）。
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公开(公告)号：US07037649B2
公开(公告)日：2006-05-02
申请号：US09771043
申请日：2001-01-26
申请人： Naoki Kimura , Tatsuo Ichihara , Shogo Moriya
发明人： Naoki Kimura , Tatsuo Ichihara , Shogo Moriya
CPC分类号： C12Q1/6837 , C12Q1/6834 , C12Q2525/197 , C12Q2523/101 , C12Q2523/313 , C12Q2525/161
摘要： A nucleic acid to be immobilized and used for hybridization of nucleic acids using an immobilized nucleic acid, which has a polymer comprising a compound having an unsaturated bond, said polymer being bonded to the 3′ end or 5′ end or both ends of the nucleic acid; a nucleic acid-immobilized substrate comprising a substrate for immobilizing a nucleic acid and the polymer-having nucleic acid immobilized on the substrate; and a method for detecting a nucleic acid by hybridization using an immobilized nucleic acid, which comprises using the nucleic acid-immobilized substrate.
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公开(公告)号：US07867708B2
公开(公告)日：2011-01-11
申请号：US11659660
申请日：2005-09-08
申请人： Tatsuo Ichihara , Mitsugu Usui
发明人： Tatsuo Ichihara , Mitsugu Usui
CPC分类号： G01N33/532 , C12N15/10 , C12Q1/682 , Y10T436/143333 , C12Q2537/149 , C12Q2525/161 , C12Q2525/155
摘要： To solve a problem occurring in the PALSAR method that a polymer would be formed in the state of unbound to a captured test gene and thus affect the quantitative characteristics as a nonspecific signal, it is intended to develop a technique whereby the polymer formation is controlled in the step of forming an assembly (polymer) of probes so that the polymer is formed exclusively on a test gene to thereby improve the sensitivity and quantitative characteristics. It is found that the polymer can be quantitatively formed and a nonspecific reaction can be inhibited by, in the step of forming a polymer by reacting plural kinds of probes having abilities to complementarily bind to each other, not adding or reacting these probes at once but starting with the reaction of a first probe in one group, and then reacting the second probe in the other group followed by the reactions of probes one by one (i.e., the first probe, the second probe, and so on).
摘要翻译： 为了解决在PALSAR方法中发生聚合物将以与捕获的测试基因无关的状态形成并因此影响作为非特异信号的定量特征的问题，旨在开发一种技术，其中聚合物形成被控制在 形成探针的组件（聚合物）的步骤，使得聚合物专门在测试基因上形成，从而提高灵敏度和定量特性。 发现可以定量地形成聚合物，并且在通过使具有彼此互补结合能力的多种探针反应形成聚合物的步骤中不能一次添加或反应这些探针来抑制非特异性反应， 从一组中的第一探针的反应开始，然后使另一组中的第二探针反应，然后逐个进行探针的反应（即，第一探针，第二探针等）。
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