公开(公告)号：US20230228732A1

公开(公告)日：2023-07-20

申请号：US18010823

申请日：2021-04-29

Applicant: Oxford Nanopore Technologies PLC

Inventor： Ping Xie ,  Justin Millis ,  Ken Healy ,  James Anthony Clarke ,  Jason Robert Hyde ,  Richard Kenneth John Wiltshire ,  Jonathan Edward McKendry ,  Robert Greasty ,  Clive Gavin Brown ,  loana Pera ,  Gurdial Singh Sanghera ,  Mark Hyland ,  Pedro Miguel Ortiz Bahamon ,  Mark David Jackson ,  Paul Raymond Mackett ,  Rhodri Rhys Davies

IPC: G01N33/487 ,  G01N27/30 ,  B82Y5/00 ,  B01L3/00

CPC classification number: G01N33/48721 ,  G01N27/308 ,  B82Y5/00 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2300/0896 ,  B01L2300/0861 ,  B01L2300/0816

Abstract: There is disclosed a nanopore support structure comprising a wall layer comprising walls defining a plurality of wells, and overhangs extending from the walls across each of the wells, the overhang defining an aperture configured to support a membrane suitable for insertion of a nanopore. There is further disclosed a nanopore sensing device comprising a nanopore support structure, and methods of manufacturing the nanopore support structure and the nanopore sensing device.

公开(公告)号：US20250033016A1

公开(公告)日：2025-01-30

申请号：US18796147

申请日：2024-08-06

Applicant: OXford Nanopore Technologies PLC

Inventor： Jason Robert Hyde ,  Pedro Miguel Ortiz Bahamon ,  Clive Gavin Brown ,  Andrew John Heron ,  Paul Raymond Mackett

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487 ,  G01N33/573

Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

公开(公告)号：US20240253004A1

公开(公告)日：2024-08-01

申请号：US18595164

申请日：2024-03-04

Applicant: Oxford Nanopore Technologies PLC

Inventor： Jason Robert Hyde ,  Pedro Miguel Ortiz Bahamon ,  Clive Gavin Brown ,  Andrew John Heron ,  Paul Raymond Mackett

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487 ,  G01N33/573

CPC classification number: B01J19/0046 ,  B01L3/5088 ,  C12Q1/6869 ,  G01N33/48721 ,  G01N33/573 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00734 ,  B01J2219/00736 ,  B01L2200/0642 ,  B01L2400/086 ,  G01N2333/974

Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

公开(公告)号：US20240253005A1

公开(公告)日：2024-08-01

申请号：US18614432

申请日：2024-03-22

Applicant: Oxford Nanopore Technologies PLC

Inventor： Jason Robert Hyde ,  Pedro Miguel Ortiz Bahamon ,  Clive Gavin Brown ,  Andrew John Heron ,  Paul Raymond Mackett

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487 ,  G01N33/573

CPC classification number: B01J19/0046 ,  B01L3/5088 ,  C12Q1/6869 ,  G01N33/48721 ,  G01N33/573 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00734 ,  B01J2219/00736 ,  B01L2200/0642 ,  B01L2400/086 ,  G01N2333/974

Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

公开(公告)号：US20220396013A1

公开(公告)日：2022-12-15

申请号：US17771179

申请日：2020-10-23

Applicant: Oxford Nanopore Technologies PLC

Inventor： Pedro Miguel Ortiz Bahamon ,  Paul Raymond Mackett ,  Robert Greasty ,  Jonathan Edward McKendry ,  Jason Robert Hyde ,  Mark David Jackson ,  Richard Kenneth John Wiltshire ,  Rhodri Rhys Davies ,  Mark Hyland ,  James Anthony Clarke ,  Gurdial Singh Sanghera

IPC: B29C43/20 ,  B01L3/00 ,  B29C43/18

Abstract: The invention relates to a method of forming a sensing device for supporting a plurality of nanopores upon an array of wells. The method involves providing a substrate, said substrate having a surface having an array of electrodes located thereon for connecting to or for configuring upon an electronic circuit. Separately, a well array structure is provided, which has an array of walls defining through-holes for defining wells. The substrate and well array structures are aligning said array of electrodes define, at least in part, a portion of the bases of respective wells at the bottom of the through holes. The resulting sensing device overcomes problems with known sensing devices by employing a substrate and/or well array structure, or hybrid thereof, that employs alternative materials or manufacturing processes.