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公开(公告)号：US20220213540A1
公开(公告)日：2022-07-07
申请号：US17605640
申请日：2020-04-22
发明人： Jeong Wook LEE , Gyoo Yeol JUNG , Chang Ha WOO , Sungho Jang , Giyoung SHIN
IPC分类号： C12Q1/6862 , C12Q1/6876
摘要： The present invention relates to a novel probe set for an isothermal one-pot reaction, and uses thereof and, particularly, provides a method for easily, accurately, and quickly diagnosing molecules, in particular, infection diseases, in the field, the method having a form applicable in the field on the basis of a nucleic acid sequence by using a one-pot reaction composition under an isothermal condition. If a molecular diagnostic platform according to the present invention is used, a target sequence can be quickly and accurately detected. Also, elements (reaction buffer, enzyme) needed in a diagnostic process using the present invention are much more simple and convenient than in a conventional antibody-based diagnosis, and, thus, the diagnostic process can be performed by a non-skilled person, and the sensitivity and speed of diagnosis can be increased, as all reactions are unified at a constant temperature without expensive equipment used in general nucleic-acid-based molecular diagnostic technology, and an amplification process is performed automatically during a reaction process.
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公开(公告)号：US20230313239A1
公开(公告)日：2023-10-05
申请号：US18192868
申请日：2023-03-30
发明人： Gyoo Yeol JUNG , Jae Seong YANG , Myung Hyun NOH , Dae Yeol YE
CPC分类号： C12P7/46 , C12N15/52 , C12N9/88 , C12Y401/01006 , C12Y402/01079 , C12N15/70 , C12N1/20 , C12N2800/101
摘要： Provided is a novel cis-aconitate synthesis enzyme, more particularly, a recombinant microorganism for producing itaconate including a cis-aconitate synthesis enzyme variant. According to the present disclosure, it was confirmed that the production and yield of itaconate were significantly increased in the recombinant microorganism for producing itaconate into which the novel cis-aconitate synthesis enzyme was introduced. In addition, it was confirmed that in the recombinant microorganism for producing itaconate of the present disclosure, a new carbon flow to itaconate was separated from the existing TCA cycle based on the activity of the corresponding enzyme. Accordingly, the novel aconitate synthesis enzyme of the present disclosure and the recombinant microorganism introduced with the aconitate synthesis enzyme can increase the economic feasibility of itaconate, and thus can be used in various industrial fields, such as synthetic resins, latexes, and food additives in which itaconate is used.
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3.发明公开公开(公告)号：US20240287551A1
公开(公告)日：2024-08-29
申请号：US18572361
申请日：2022-02-28
发明人： Gyoo Yeol JUNG , Dae Yeol YE , Myung Hyun NOH , Myeong Won MIN
CPC分类号： C12P7/44 , C12N9/1029 , C12N9/90 , C12N15/70 , C12Y203/01182 , C12Y503/03006
摘要： The present invention relates to a novel metabolic pathway for producing itaconic acid and a recombinant microorganism, for producing itaconic acid, into which the metabolic pathway is introduced. It has been confirmed that the recombinant microorganism, for producing itaconic acid, into which the novel itaconic acid metabolic pathway is introduced, according to the present invention, significantly increases the production and yield of itaconic acid. In addition, as the recombinant microorganism for producing itaconic acid of the present invention uses an intermediate material of glycolysis rather than an intermediate material of the TCA cycle, the recombinant microorganism can further increase the production yield of itaconic acid through further improvement. Accordingly, the novel metabolic pathway for producing itaconic acid and the recombinant microorganism into which the metabolic pathway is introduced can increase the economic feasibility of itaconic acid, and thus can be variously used in industrial fields for synthetic resins, latexes, food additives, and the like in which itaconic acid is utilized.
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