公开(公告)号：US20200049689A1

公开(公告)日：2020-02-13

申请号：US16488754

申请日：2018-02-26

Applicant: President and Fellows of Harvard College

Inventor： Ben M. Maoz ,  Anna Herland ,  Olivier F. Henry ,  Kevin K. Parker ,  Donald E. Ingber

IPC: G01N33/483 ,  C12M1/12 ,  C12M3/06 ,  C12M1/34 ,  G01N33/487 ,  B01L3/00

Abstract: An organ-on-chip device for monitoring a biological function and including a membrane layer located at an interface region between a top microchannel and the a microchannel. The membrane includes a first type of cells forming a barrier between the top microchannel and the bottom microchannel. The device further includes a top layer having a first plurality of transendothelial electrical resistance (TEER) measurement electrodes for enabling direct monitoring of cell function and electrical activity of the first type of cells on the membrane. The device also has a multi-electrode array (MEA) layer with a second plurality of TEER measurement electrodes for enabling direct monitoring of cell function and electrical activity of a second type of cells on the MEA layer.

公开(公告)号：US20170158997A1

公开(公告)日：2017-06-08

申请号：US15367865

申请日：2016-12-02

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Geraldine A. Hamilton ,  Kyung-Jin Jang ,  Suzzette Haney ,  Payal Patel ,  Anna Herland ,  Joshua Isaac Nielsen Resnikoff

IPC: C12M3/06 ,  C12N5/00 ,  C12M1/12 ,  C12N5/071 ,  C12M1/00

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M23/20 ,  C12M25/02 ,  C12M25/14 ,  C12N5/0068 ,  C12N5/067 ,  C12N2502/28 ,  C12N2531/00 ,  C12N2533/54 ,  C12N2533/90

Abstract: Provided herein relates to devices for simulating a function of a tissue and methods of using the same. In some embodiments, the devices can be used to simulate a function of a human liver tissue. In some embodiments, the devices can be used to simulate a function of a dog liver tissue. Endothelial cell culture media for long-term culture of endothelial cells are also described herein.

公开(公告)号：US11371022B2

公开(公告)日：2022-06-28

申请号：US16076857

申请日：2017-02-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Tae-Eun Park ,  Anna Herland ,  Edward Anthony Fitzgerald ,  Donald Elliot Ingber

IPC: C12N5/071 ,  C12M3/06 ,  C12N5/00 ,  G01N27/403

Abstract: The invention relates to a method of creating a human blood-brain barrier (BBB) model from the differentiation of human pluripotent stem cells (hPSCs), wherein the BBB exhibits sustained transendothelial electrical resistances (TEER) over 2000 Ω·cm2 for at least 3 days after seeding.

公开(公告)号：US20220089989A1

公开(公告)日：2022-03-24

申请号：US17540619

申请日：2021-12-02

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Andries Van der Meer ,  Anna Herland

IPC: C12M3/06 ,  C12M1/12 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  C12N5/079 ,  G01N33/50

Abstract: Systems and methods for producing and using a body having a first structure defining a first chamber, a second structure defining a second chamber, a membrane located at an interface region between the first chamber and the second chamber to separate the first chamber from the second chamber. The first chamber comprises a first permeable matrix disposed therein and the first permeable matrix comprises at least one or a plurality of lumens each extending therethrough, which is optionally lined with at least one layer of cells. The second chamber can comprise cells cultured therein. The systems and methods described herein can be used for various applications, including, e.g., growth and/or differentiation of primary cells, and/or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents). The systems and methods can also permit co-cultures of two or more different cell types.