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公开(公告)号：US06376248B1
公开(公告)日：2002-04-23
申请号：US09039780
申请日：1998-03-16
申请人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
发明人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
IPC分类号： C12N1588
CPC分类号： B82Y5/00 , A61K9/1272 , A61K47/54 , A61K47/543 , A61K47/645 , A61K47/6949 , A61K48/00 , A61K48/0025 , A61K48/0041 , C07K1/1077 , C07K7/06 , C07K7/08 , C07K14/005 , C12N15/87 , C12N2740/16322
摘要： The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
摘要翻译： 本发明提供了可用于转染包含与肽的核酸复合物的真核细胞的组合物，其中肽任选地与核酸结合基团共价连接，并且阳离子脂质或树状聚合物作为转染剂。 本发明还提供其中肽与转染剂（脂质，阳离子脂质或树枝状大分子）共价连接的转染组合物。 在转染组合物中包含肽或修饰肽或将肽共价连接到转染试剂导致增强的转染效率。 还公开了制备转染组合物的方法和使用这些转染组合物作为细胞内递送剂和细胞外靶向剂的方法。
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公开(公告)号：US08058068B2
公开(公告)日：2011-11-15
申请号：US10200879
申请日：2002-07-23
申请人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
发明人： Pamela Hawley-Nelson , Jianqing Lan , PoJen Shih , Joel A. Jessee , Kevin P. Schifferli , Gulilat Gebeyehu , Valentina C. Ciccarone , Krista L. Evans
IPC分类号： C12N15/88 , C07K14/00 , C07K14/025 , C07H21/02 , C07H21/04
CPC分类号： B82Y5/00 , A61K9/1272 , A61K38/00 , A61K47/543 , A61K47/645 , A61K47/6911 , A61K48/00 , A61K48/0025 , A61K48/0041 , C07K1/1077 , C07K7/06 , C07K7/08 , C07K14/005 , C07K2319/09 , C12N15/87 , C12N15/88 , C12N2740/16322 , C12N2810/405 , C12N2810/60 , C12N2810/6018 , C12N2810/6054 , C12N2810/6081 , Y10T428/2984
摘要： The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
摘要翻译： 本发明提供了可用于转染包含与肽的核酸复合物的真核细胞的组合物，其中肽任选地与核酸结合基团共价连接，并且阳离子脂质或树状聚合物作为转染剂。 本发明还提供其中肽与转染剂（脂质，阳离子脂质或树枝状大分子）共价连接的转染组合物。 在转染组合物中包含肽或修饰肽或将肽共价连接到转染试剂导致增强的转染效率。 还公开了制备转染组合物的方法和使用这些转染组合物作为细胞内递送剂和细胞外靶向剂的方法。
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公开(公告)号：US5334761A
公开(公告)日：1994-08-02
申请号：US937508
申请日：1992-08-28
申请人： Gulilat Gebeyehu , Joel A. Jessee , Valentina C. Ciccarone , Pamela Hawley-Nelson , Anna Chytil
发明人： Gulilat Gebeyehu , Joel A. Jessee , Valentina C. Ciccarone , Pamela Hawley-Nelson , Anna Chytil
IPC分类号： C12N5/10 , A61K9/127 , A61K47/18 , C07C27/28 , C07C217/28 , C07C219/06 , C07C219/08 , C07C237/10 , C07C323/25 , C07C233/36
CPC分类号： C07C237/10 , A61K47/48046 , A61K9/1272 , C07C217/28
摘要： The present invention discloses cationic lipids useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Also disclosed are lipids useful both for the delivery of macromolecules and also useful as intermediates for making other such lipids.
摘要翻译： 本发明公开了用于制备用于将大分子和其它化合物递送到细胞中的脂质聚集体的阳离子脂质。 它们对细胞的DNA依赖性转化特别有用。 还公开了可用于递送大分子的脂质，也可用作制备其它这种脂质的中间体。
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公开(公告)号：US07217566B2
公开(公告)日：2007-05-15
申请号：US10805536
申请日：2004-03-22
CPC分类号： C12N5/00 , C12N2501/998 , C12N2510/02
摘要： The present invention provides novel cell lines that may have improved adhesive qualities, transgene expression level, growth rate, and/or growth rate in serum free medium or even chemically defined compared to cells of the prior art.
摘要翻译： 本发明提供了与现有技术的细胞相比，可以具有改善的无血清培养基中的粘合剂质量，转基因表达水平，生长速率和/或生长速率甚至化学上定义的新型细胞系。
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公开(公告)号：US06451579B1
公开(公告)日：2002-09-17
申请号：US09361740
申请日：1999-07-28
IPC分类号： C12N700
CPC分类号： C12N15/86 , C12N2770/36143 , C12N2830/002 , C12N2830/003 , C12N2840/20 , C12N2840/203
摘要： The present invention describes cells and constructs for a regulated viral (e.g. alphavirus) expression system, where gene expression is controlled by controlling expression of replicases or nonstructural proteins and/or controlling the amount of such proteins introduced in a cell, which in turn regulates RNA replication and subsequently gene expression. Particularly, this system takes advantage of the high level expression of the alphavirus systems for recombinant protein production and allows for large scale applications without biosafety concerns.
摘要翻译： 本发明描述了用于受调节病毒（例如甲病毒）表达系统的细胞和构建体，其中通过控制复制酶或非结构蛋白质的表达和/或控制在细胞中引入的这种蛋白质的量来控制基因表达，其依次调节RNA 复制和随后的基因表达。 特别地，该系统利用了甲病毒系统用于重组蛋白质生产的高水平表达，并允许在没有生物安全性的情况下进行大规模应用。
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公开(公告)号：US5834439A
公开(公告)日：1998-11-10
申请号：US782783
申请日：1997-01-13
IPC分类号： C12N15/09 , A61K9/127 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/54 , C12N5/10 , C12N15/87 , A61K31/13 , C07C211/13
CPC分类号： C12N15/87 , A61K9/1272 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/5449 , C07F9/5463 , Y10S435/829
摘要： The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.
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公开(公告)号：US06399663B1
公开(公告)日：2002-06-04
申请号：US09648492
申请日：2000-08-25
IPC分类号： A61K9127
CPC分类号： C12N15/87 , A61K9/1272 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/5449 , C07F9/5463 , Y10S435/829
摘要： The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.
摘要翻译： 本发明公开了用于制备用于将大分子和其它化合物递送到细胞中的脂质聚集体的高填充聚阳离子铵，锍和鏻脂质化合物。 它们对细胞的DNA依赖性转化特别有用。 还公开了其制备和用作细胞内递送剂的方法。
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公开(公告)号：US6110916A
公开(公告)日：2000-08-29
申请号：US187676
申请日：1998-11-06
IPC分类号： C12N15/09 , A61K9/127 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/54 , C12N5/10 , C12N15/87 , A61K31/495 , C07D241/04
CPC分类号： C12N15/87 , A61K9/1272 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/5449 , C07F9/5463 , Y10S435/829
摘要： The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.
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公开(公告)号：US20080207776A1
公开(公告)日：2008-08-28
申请号：US11931075
申请日：2007-10-31
IPC分类号： A61K47/16 , A61K47/14 , A61K47/08 , C07C211/63 , C07C381/00 , C07C229/26 , C07C237/04 , C07C271/08
CPC分类号： C12N15/87 , A61K9/1272 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/5449 , C07F9/5463 , Y10S435/829
摘要： The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.
摘要翻译： 本发明公开了用于制备用于将大分子和其它化合物递送到细胞中的脂质聚集体的高填充聚阳离子铵，锍和鏻脂质化合物。 它们对细胞的DNA依赖性转化特别有用。 还公开了其制备和用作细胞内递送剂的方法。
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公开(公告)号：US5674908A
公开(公告)日：1997-10-07
申请号：US171232
申请日：1993-12-20
IPC分类号： C12N15/09 , A61K9/127 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/54 , C12N5/10 , C12N15/87 , A61K31/14
CPC分类号： C12N15/87 , A61K9/1272 , C07C211/63 , C07C217/40 , C07C233/36 , C07C381/12 , C07F9/5449 , C07F9/5463 , Y10S435/829
摘要： The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.
摘要翻译： 本发明公开了用于制备用于将大分子和其它化合物递送到细胞中的脂质聚集体的高填充聚阳离子铵，锍和鏻脂质化合物。 它们对细胞的DNA依赖性转化特别有用。 还公开了其制备和用作细胞内递送剂的方法。
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