公开(公告)号：US20240002936A1

公开(公告)日：2024-01-04

申请号：US18034876

申请日：2021-11-01

申请人： Paola ARLOTTA ,  Aviv REGEV ,  President and Fellows of Harvard College ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Paola Arlotta ,  Joshua Z. Levin ,  Amanda J. Kedaigle ,  Aviv Regev ,  Bruna Da Silveira Paulsen ,  Silvia Velasco ,  Martina Pigoni

IPC分类号： C12Q1/6883 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  G01N33/5088 ,  C12Q2600/156

摘要： The present disclosure is directed to methods of screening for pathological mechanisms in neurodevelopmental and neuropsychological disorders using brain organoids. The present disclosure is also directed to methods of screening agents using the brain organoids.

公开(公告)号：US20230324371A1

公开(公告)日：2023-10-12

申请号：US18021156

申请日：2021-08-12

申请人： Paola ARLOTTA ,  Mario L. SUVA ,  Aviv REGEV ,  Kwanghun GHUNG ,  Vamsi MANGENA ,  Rafaela SARTORE ,  President and Fellows of Harvard College ,  The Broad Institute, Inc. ,  The General Hospital Corporation ,  Massachusetts Institute of Technology

发明人： Paola Arlotta ,  Mario L. Suva ,  Aviv Regev ,  Kwanghun Chung ,  Vamsi Mangena ,  Rafaela Sartore

IPC分类号： G01N33/50 ,  C12N5/071

CPC分类号： G01N33/5082 ,  G01N33/5011 ,  G01N33/5023 ,  C12N5/0697 ,  C12N2513/00 ,  C12N2503/04

摘要： Compositions and systems comprising a dorsal forebrain organoid having a core comprising less than 25% apoptotic or hypoxic cells and one or more tumor cells in the organoid.

公开(公告)号：US20210172017A1

公开(公告)日：2021-06-10

申请号：US17163015

申请日：2021-01-29

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Xin Jin ,  Paola Arlotta ,  Aviv Regev ,  Feng Zhang ,  Sean Simmons

IPC分类号： C12Q1/6883 ,  C12Q1/6897 ,  C12N15/90

摘要： Described herein are methods and uses thereof for in vivo evaluating functions of multiple genes in parallel by combining in utero genetic perturbation of progenitor cells and single-cell transcriptomic profiling of progeny cells in animals. These methods can be used, among other things, to reveal in vivo gene functions in a cell type-specific manner.

公开(公告)号：US20220235322A1

公开(公告)日：2022-07-28

申请号：US17615579

申请日：2020-05-29

申请人： President and Fellows of Harvard College ,  The Broad Institute, Inc.

发明人： Silvia Velasco ,  Paola Arlotta

IPC分类号： C12N5/079 ,  C12N5/071 ,  G01N33/50

摘要： The present disclosure is directed to methods of producing dorsal forebrain organoids having cores with a very low incident of apoptotic and hypoxic cells and having highly similar cell types and cell type prevalence. The present disclosure is also directed to compositions comprising such organoids and the use of such organoids for the screening of agents.

公开(公告)号：US20190002835A1

公开(公告)日：2019-01-03

申请号：US16067815

申请日：2016-12-30

申请人： President and Fellows of Harvard College

发明人： Giorgia Quadrato ,  Paola Arlotta

IPC分类号： C12N5/071 ,  C12N5/079

摘要： Disclosed herein are 3-D neural tissue structures or “brain organoids” created from human pluripotent cells (e.g., stem cells) differentiated into neuronal cell types that include cortical and subcortical neuronal subtypes along with sensory cells. Also disclosed herein are methods for the in vitro generation of 3-D neural tissue structures capable of sensory perception, methods for generating a “brain organoid-machine interface” (BOMI), and methods for screening of molecular, cellular and network-level defects associated with complex mental diseases through use of patient-derived induced pluripotent stem cells.

公开(公告)号：US20240076630A1

公开(公告)日：2024-03-07

申请号：US18231190

申请日：2023-08-07

申请人： President and Fellows of Harvard College

发明人： Giorgia Quadrato ,  Paola Arlotta

IPC分类号： C12N5/071 ,  C12N5/079 ,  C12N5/0793

CPC分类号： C12N5/0697 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2533/90

摘要： Disclosed herein are 3-D neural tissue structures or “brain organoids” created from human pluripotent cells (e.g., stem cells) differentiated into neuronal cell types that include cortical and subcortical neuronal subtypes along with sensory cells. Also disclosed herein are methods for the in vitro generation of 3-D neural tissue structures capable of sensory perception, methods for generating a “brain organoid-machine interface” (BOMI), and methods for screening of molecular, cellular and network-level defects associated with complex mental diseases through use of patient-derived induced pluripotent stem cells.

公开(公告)号：US20120251506A1

公开(公告)日：2012-10-04

申请号：US13474786

申请日：2012-05-18

申请人： Jeffrey D. Macklis ,  Paola Arlotta ,  Bradley J. Molyneaux

发明人： Jeffrey D. Macklis ,  Paola Arlotta ,  Bradley J. Molyneaux

IPC分类号： C12N5/0793 ,  C12Q1/68 ,  A61P25/00 ,  A61K35/30

CPC分类号： C12Q1/6876 ,  C12N5/0619 ,  C12N2501/60 ,  C12Q2600/158

摘要： The invention relates to methods for isolating and purifying specific types of neurons, such as cortical or other projection neurons including corticospinal motor neurons, subcerebral projection neurons, and callosal projection neurons. The invention also relates to genes that are specific for particular neuronal subtypes, and the use of such genes in genetic/molecular control of cell development. The isolated cells and subtype-specific genes also have uses in diagnostics, therapeutics, and screening assays for pharmaceutical molecules.

公开(公告)号：US20120020929A1

公开(公告)日：2012-01-26

申请号：US13009595

申请日：2011-01-19

申请人： Jeffrey D. Macklis ,  Paola Arlotta ,  Bradley J. Molyneaux

发明人： Jeffrey D. Macklis ,  Paola Arlotta ,  Bradley J. Molyneaux

IPC分类号： A61K35/30 ,  A61P25/00 ,  G01N33/566 ,  C40B30/04 ,  C12N5/0793 ,  C12Q1/68

CPC分类号： C12Q1/6876 ,  C12N5/0619 ,  C12N2501/60 ,  C12Q2600/158

摘要： The invention relates to methods for isolating and purifying specific types of neurons, such as cortical or other projection neurons including corticospinal motor neurons, subcerebral projection neurons, and callosal projection neurons. The invention also relates to genes that are specific for particular neuronal subtypes, and the use of such genes in genetic/molecular control of cell development. The isolated cells and subtype-specific genes also have uses in diagnostics, therapeutics, and screening assays for pharmaceutical molecules.

摘要翻译： 本发明涉及用于分离和纯化特定类型的神经元的方法，例如包括皮质脊髓运动神经元，脑下投影神经元和胼cal体投影神经元的皮质或其他投影神经元。 本发明还涉及对特定神经元亚型特异的基因，以及这些基因在细胞发育的遗传/分子控制中的用途。 分离的细胞和亚型特异性基因也可用于药物分子的诊断，治疗和筛选测定。

公开(公告)号：US20050266563A1

公开(公告)日：2005-12-01

申请号：US11085839

申请日：2005-03-21

申请人： Jeffrey Macklis ,  Paola Arlotta ,  Bradley Molyneaux

发明人： Jeffrey Macklis ,  Paola Arlotta ,  Bradley Molyneaux

IPC分类号： C12N5/0793 ,  C12N15/85 ,  C12Q1/68 ,  C12N5/08

CPC分类号： C12Q1/6876 ,  C12N5/0619 ,  C12N2501/60 ,  C12Q2600/158

摘要： The invention relates to methods for isolating and purifying specific types of neurons, such as cortical or other projection neurons including corticospinal motor neurons, subcerebral projection neurons, and callosal projection neurons. The invention also relates to genes that are specific for particular neuronal subtypes, and the use of such genes in genetic/molecular control of cell development. The isolated cells and subtype-specific genes also have uses in diagnostics, therapeutics, and screening assays for pharmaceutical molecules.

摘要翻译： 本发明涉及用于分离和纯化特定类型的神经元的方法，例如包括皮质脊髓运动神经元，脑下投影神经元和胼cal体投影神经元的皮质或其他投影神经元。 本发明还涉及对特定神经元亚型特异的基因，以及这些基因在细胞发育的遗传/分子控制中的用途。 分离的细胞和亚型特异性基因也可用于药物分子的诊断，治疗和筛选测定。