公开(公告)号：US20230324371A1

公开(公告)日：2023-10-12

申请号：US18021156

申请日：2021-08-12

申请人： Paola ARLOTTA ,  Mario L. SUVA ,  Aviv REGEV ,  Kwanghun GHUNG ,  Vamsi MANGENA ,  Rafaela SARTORE ,  President and Fellows of Harvard College ,  The Broad Institute, Inc. ,  The General Hospital Corporation ,  Massachusetts Institute of Technology

发明人： Paola Arlotta ,  Mario L. Suva ,  Aviv Regev ,  Kwanghun Chung ,  Vamsi Mangena ,  Rafaela Sartore

IPC分类号： G01N33/50 ,  C12N5/071

CPC分类号： G01N33/5082 ,  G01N33/5011 ,  G01N33/5023 ,  C12N5/0697 ,  C12N2513/00 ,  C12N2503/04

摘要： Compositions and systems comprising a dorsal forebrain organoid having a core comprising less than 25% apoptotic or hypoxic cells and one or more tumor cells in the organoid.

公开(公告)号：US20240002936A1

公开(公告)日：2024-01-04

申请号：US18034876

申请日：2021-11-01

申请人： Paola ARLOTTA ,  Aviv REGEV ,  President and Fellows of Harvard College ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Paola Arlotta ,  Joshua Z. Levin ,  Amanda J. Kedaigle ,  Aviv Regev ,  Bruna Da Silveira Paulsen ,  Silvia Velasco ,  Martina Pigoni

IPC分类号： C12Q1/6883 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  G01N33/5088 ,  C12Q2600/156

摘要： The present disclosure is directed to methods of screening for pathological mechanisms in neurodevelopmental and neuropsychological disorders using brain organoids. The present disclosure is also directed to methods of screening agents using the brain organoids.

公开(公告)号：US20230235400A1

公开(公告)日：2023-07-27

申请号：US17928696

申请日：2021-06-04

申请人： The General Hospital Corporation ,  Children's Medical Center Corporation ,  President and Fellows of Harvard College ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Vamsi Mootha ,  Vijay Sankaran ,  Caleb Lareau ,  Melissa Walker ,  Leif Ludwig ,  Aviv Regev

IPC分类号： C12Q1/6883 ,  G16B30/00 ,  G16B20/00

CPC分类号： C12Q1/6883 ,  G16B30/00 ,  G16B20/00 ,  C12Q1/6869

摘要： Described herein are methods of determining segregation dynamics of mitochondrial DNA herein. Also described herein are methods of diagnosing, prognosing, and/or monitoring a mitochondrial disease.

公开(公告)号：US20200208114A1

公开(公告)日：2020-07-02

申请号：US16709710

申请日：2019-12-10

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The General Hospital Corporation ,  President and Fellows of Harvard College

发明人： Ninib Baryawno ,  Dariusz Przybylski ,  Monika Kowalczyk ,  Aviv Regev ,  David T. Scadden

IPC分类号： C12N5/0775 ,  G01N33/50 ,  C12N15/11 ,  C12N9/22 ,  A61K35/28 ,  C12Q1/6886

摘要： Described herein are signatures that characterize a particular stromal cell state, type, and/or subtype. In some embodiments, the signatures can characterize a dysfunctional stromal cell. In some embodiments, the signatures can be used to diagnose, treat, and/or prevent a disease. In some embodiments, the signatures can characterize remodeling in a bone marrow microenvironment. Also described herein are cell populations having a specific signature and modulated cells that can be modulate to have a specific signature.

公开(公告)号：US20180289709A1

公开(公告)日：2018-10-11

申请号：US15782674

申请日：2017-10-12

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE GENERAL HOSPITAL CORPORATION ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Aviv Regev ,  Ido Amit ,  Nir Hacohen ,  Manuel Garber ,  Nicolas Chevrier

IPC分类号： A61K31/519 ,  A61K31/688 ,  C12Q1/6883

摘要： The present invention relates to methods of decreasing inflammation by inhibiting polo-like kinase (Plk)

公开(公告)号：US11209440B2

公开(公告)日：2021-12-28

申请号：US15245748

申请日：2016-08-24

申请人： THE BROAD INSTITUTE, INC. ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Vijay Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  A61K48/00 ,  C12N15/68 ,  C12N15/86 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20210246503A1

公开(公告)日：2021-08-12

申请号：US17251451

申请日：2019-06-11

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Children's Medical Center Corporation ,  President and Fellows of Harvard College

发明人： Leif S. Ludwig ,  Caleb A. Lareau ,  Jacob C. Ulirsch ,  Aviv Regev ,  Vijay G. Sankaran ,  Jason Buenrostro ,  Christoph Muus

IPC分类号： C12Q1/6869 ,  C12Q1/6827 ,  A61K45/06 ,  C12Q1/6886

摘要： Embodiments disclosed herein provide methods of using somatic mutations in mitochondrial genomes to retrospectively infer cell lineages in native contexts and to serve as genetic barcodes to measure clonal dynamics in complex cellular populations. Further, somatic mutations in mitochondrial DNA (mtDNA) are tracked by single cell genomic approaches for simultaneous analysis of single cell lineage and state. Applicants further show that mitochondrial mutations can be readily detected with contemporary single cell transcriptomic and epigenomic technologies to concomitantly capture gene expression profiles and chromatin accessibility, respectively.

公开(公告)号：US20200248255A1

公开(公告)日：2020-08-06

申请号：US16782044

申请日：2020-02-04

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Jason Daniel Buenrostro ,  Sai Ma ,  Aviv Regev

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12Q1/6853

摘要： Provided herein include methods and compositions for analyzing nucleic acid in individual cells. In some embodiments, the methods herein include generating, within individual cells, fragmented cellular genomic DNA and cDNA copies of cellular RNA molecules, barcoding the fragmented genomic DNA and the cDNA within each cell such that the genomic DNA and the cDNA from the same cell receive the same unique barcode sequence, isolating the barcoded genomic DNA and cDNA, and characterizing one or more features of the individual cells based, at least in part, on sequencing of the isolated barcoded genomic DNA and the cDNA.

公开(公告)号：US20190218276A1

公开(公告)日：2019-07-18

申请号：US16087036

申请日：2016-10-27

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Aviv Regev ,  Feng Zhang ,  Naomi Habib ,  Yinqing Li ,  Matthias Heidenreich ,  Lukasz Swiech ,  Anindita Basu ,  David Weitz ,  Inbal Avraham Davidi

IPC分类号： C07K16/10 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6869

CPC分类号： C07K16/1036 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2537/165 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12Q2565/629

摘要： Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are limited. Here, Applicants developed Nuc-seq, Div-Seq, and Dronc-Seq. Div-seq combines Nuc-Seq, a scalable single nucleus RNA-Seq method, with EdU-mediated labeling of proliferating cells. Nuc-Seq can sensitively identify closely related cell types within the adult hippocampus. Div-Seq can track transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead. Finally, Applicants found rare adult newborn GABAergic neurons in the spinal cord, a non-canonical neurogenic region. Taken together, Nuc-Seq, Div-Seq and Dronc-Seq allow for unbiased analysis of any complex tissue.

公开(公告)号：US20150368719A1

公开(公告)日：2015-12-24

申请号：US14846219

申请日：2015-09-04

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Aviv Regev ,  Alexander K. Shalek ,  Rahul Satija ,  Hongkun Park

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  C12Q2600/158 ,  G01N33/5047 ,  G01N33/6893 ,  G01N2800/24

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences dendritic cell (DC) response(s), for example, dendritic cell maturation, dendritic cell antiviral response(s) and/or dendritic cell inflammatory response(s), as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences dendritic cell response(s) in a variety of therapeutic and/or diagnostic indications.

摘要翻译： 本发明一般涉及用于鉴定调节，控制或以其他方式影响树突细胞（DC）反应的调节网络的组合物和方法，例如树突状细胞成熟，树突细胞抗病毒应答和/或树突状细胞炎症 响应以及利用调节，控制或以其他方式影响各种治疗和/或诊断适应症中的树突状细胞反应的调节网络的组合物和方法。