公开(公告)号：US06514481B1

公开(公告)日：2003-02-04

申请号：US09718825

申请日：2000-11-22

申请人： Paras N. Prasad ,  Earl J. Bergey ,  Charles Liebow ,  Laurent Levy

发明人： Paras N. Prasad ,  Earl J. Bergey ,  Charles Liebow ,  Laurent Levy

IPC分类号： A61B5055

CPC分类号： B82Y5/00 ,  A61K47/64 ,  A61K47/6923 ,  A61N1/406 ,  A61N2/00 ,  Y10T436/24

摘要： The present invention provides novel nanosized particles termed as “nanoclinics” for therapeutic and/or diagnostic use. The particles have a core made of a therapeutic or diagnostic material surrounded by a shell. Further, the particles contain a targeting agent on the surface of the shell for specific recognition of targeted cells. A method is also provided for lysis of cells using DC magnetic field. Further, the present invention also provides a method for fabrication of nanoclinics that can target and lyse specific cells such as cancer cells.

摘要翻译： 本发明提供用于治疗和/或诊断用途的称为“纳米细胞学”的新型纳米尺寸颗粒。 颗粒具有由壳包围的治疗或诊断材料制成的芯。 此外，颗粒在壳的表面上含有靶向剂，用于特异性识别靶细胞。 还提供了使用DC磁场裂解细胞的方法。 此外，本发明还提供了可以靶向和裂解诸如癌细胞的特定细胞的纳米抗生素的制造方法。

公开(公告)号：US20110288234A1

公开(公告)日：2011-11-24

申请号：US12918232

申请日：2009-02-19

申请人： Ravindra K. Pandey ,  Lalit N. Goswami ,  Allan Oseroff ,  Stephanie Pincus ,  Janet Morgan ,  Paras N. Prasad ,  Earl J. Bergey

发明人： Ravindra K. Pandey ,  Lalit N. Goswami ,  Allan Oseroff ,  Stephanie Pincus ,  Janet Morgan ,  Paras N. Prasad ,  Earl J. Bergey

IPC分类号： C08G77/38 ,  C08F130/08 ,  C08G77/388 ,  B82Y5/00

CPC分类号： A61K49/0032 ,  A61K41/0071 ,  A61K49/0093 ,  A61K51/1255

摘要： A nanoparticle including a polysiloxane base having an exterior surface and having a photosensitizer at least partly exposed at its exterior surface, said photosensitizer being secured to the exterior surface by loading the photosensitizer onto the surface after formation of the polysiloxane base of the nanoparticle. The nanoparticle may have tumor targeting moieties and may be post loaded with cyanine dye. The nanoparticle preferably includes post loaded moieties providing at least two of tumor specificity, photodynamic properties and imaging capabilities and the photosensitizer is tagged with a radioisotope. A method for preparation of the nanoparticle is also provided.

摘要翻译： 包含具有外表面并具有至少部分地在其外表面暴露的光敏剂的聚硅氧烷基的纳米颗粒，所述光敏剂通过在形成纳米颗粒的聚硅氧烷基体之后将光敏剂加载到表面上而固定到外表面。 纳米颗粒可以具有肿瘤靶向部分，并且可以用花青染料后载。 纳米颗粒优选包括提供肿瘤特异性，光动力学特性和成像能力的至少两种的后加载部分，并且用放射性同位素标记光敏剂。 还提供了制备纳米颗粒的方法。

公开(公告)号：US20100125100A1

公开(公告)日：2010-05-20

申请号：US12555609

申请日：2009-09-08

申请人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

发明人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

IPC分类号： A61K31/7088 ,  A61P43/00

CPC分类号： C12N15/111 ,  A61K9/5115 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/32 ,  Y10S977/773

摘要： Provided are methods and compositions for inhibiting expression of one or more target genes. The compositions contain RNA polynucleotides that can inhibit expression of a target gene via RNA interference (RNAi) electrostatically complexed with surface functionalized gold nanorods (GNRs). The RNA polynucleotides are not covalently bound to the surface functionalized GNRs. The method involves inhibiting expression of a target gene in an individual. The method is performed by administering to the individual an effective amount of a composition containing surface functionalized GNRs electrostatically complexed with RNA polynucleotides, such as siRNA, that can inhibit expression of the target gene via RNAi. The siRNA is not covalently bound to the surface functionalized GNRs.

摘要翻译： 提供了用于抑制一种或多种靶基因表达的方法和组合物。 组合物含有可通过与表面官能化金纳米棒（GNR）静电复合的RNA干扰（RNAi）抑制靶基因表达的RNA多核苷酸。 RNA多核苷酸不共价结合表面官能化的GNRs。 该方法涉及抑制个体中靶基因的表达。 该方法通过向个体施用有效量的含有可以通过RNAi抑制靶基因表达的RNA多核苷酸（例如siRNA）静电复合的表面官能化GNR的组合物来进行。 siRNA不与表面官能化的GNR共价结合。

公开(公告)号：US08035016B2

公开(公告)日：2011-10-11

申请号：US12555609

申请日：2009-09-08

申请人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

发明人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

IPC分类号： A61P43/00 ,  C12N15/113

CPC分类号： C12N15/111 ,  A61K9/5115 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/32 ,  Y10S977/773

摘要： Provided are methods and compositions for inhibiting expression of one or more target genes. The compositions contain RNA polynucleotides that can inhibit expression of a target gene via RNA interference (RNAi) electrostatically complexed with surface functionalized gold nanorods (GNRs). The RNA polynucleotides are not covalently bound to the surface functionalized GNRs. The method involves inhibiting expression of a target gene in an individual. The method is performed by administering to the individual an effective amount of a composition containing surface functionalized GNRs electrostatically complexed with RNA polynucleotides, such as siRNA, that can inhibit expression of the target gene via RNAi. The siRNA is not covalently bound to the surface functionalized GNRs.

摘要翻译： 提供了用于抑制一种或多种靶基因表达的方法和组合物。 组合物含有可通过与表面官能化金纳米棒（GNR）静电复合的RNA干扰（RNAi）抑制靶基因表达的RNA多核苷酸。 RNA多核苷酸不共价结合表面官能化的GNRs。 该方法涉及抑制个体中靶基因的表达。 该方法通过向个体施用有效量的含有可以通过RNAi抑制靶基因表达的RNA多核苷酸（例如siRNA）静电复合的表面官能化GNR的组合物来进行。 siRNA不与表面官能化的GNR共价结合。

公开(公告)号：US06287605B1

公开(公告)日：2001-09-11

申请号：US09293758

申请日：1999-04-16

申请人： Daniel Malamud ,  Thandavarayan Nagashunmugam ,  Harvey M. Friedman ,  William Abrams ,  Earl J. Bergey

发明人： Daniel Malamud ,  Thandavarayan Nagashunmugam ,  Harvey M. Friedman ,  William Abrams ,  Earl J. Bergey

IPC分类号： A61K3537

CPC分类号： A61L2/0082 ,  A61K31/70 ,  A61K38/1709 ,  A61K45/06 ,  Y10S435/975

摘要： The invention includes compositions comprising substantially purified salivary glycoproteins and carbohydrate ligands thereof which are useful in methods for the treatment and prevention of HIV-1 infection. The invention also includes methods for the treatment and prevention of HIV-1 infection comprising contacting a composition of the invention with a human patient. Additionally, the invention includes antibodies and kits useful in the treatment and prevention of HIV-1 infection.

摘要翻译： 本发明包括可用于治疗和预防HIV-1感染的方法的基本上纯化的唾液糖蛋白及其碳水化合物配体的组合物。 本发明还包括用于治疗和预防HIV-1感染的方法，包括将本发明的组合物与人类患者接触。 另外，本发明包括可用于治疗和预防HIV-1感染的抗体和试剂盒。

公开(公告)号：US07364754B2

公开(公告)日：2008-04-29

申请号：US10764677

申请日：2004-01-26

申请人： Paras Prasad ,  Indrajit Roy ,  Earl J. Bergey ,  Tymish Y. Ohulchansky ,  Haridas Pudavar

发明人： Paras Prasad ,  Indrajit Roy ,  Earl J. Bergey ,  Tymish Y. Ohulchansky ,  Haridas Pudavar

IPC分类号： A61K9/14 ,  A61K31/57

CPC分类号： A61K9/5115 ,  A61K31/555 ,  A61K41/0057 ,  A61K41/0071 ,  B82Y5/00

摘要： The present invention provides methods and compositions for photodynamic therapy. The composition comprises ceramic nanoparticles in which a photosensitive drug/dye is entrapped. The ceramic nanoparticles are made by formation of a micellar composition of the dye. The ceramic material is added to the micellar composition and the ceramic nanoparticles are precipitated by alkaline hydrolysis. The precipitated nanoparticles in which the photosensitive dye/drug is entrapped can be isolated by dialysis. The resulting drug doped nanoparticles are spherical, highly monodispersed, and stable in aqueous system. Irradiation with light of suitable wavelength of the photosensitizing drug entrapped inside nanoparticles resulted in generation of singlet oxygen, which was able to diffuse out through the pores of the ceramic matrix. The drug loaded ceramic nanoparticles of the present invention can be used as drug carriers for photodynamic therapy.

摘要翻译： 本发明提供光动力疗法的方法和组合物。 该组合物包含其中包含光敏药物/染料的陶瓷纳米颗粒。 通过形成染料的胶束组合物制备陶瓷纳米颗粒。 将陶瓷材料加入到胶束组合物中，并通过碱水解沉淀陶瓷纳米颗粒。 可以通过透析分离感光性染料/药物被捕获的沉淀的纳米粒子。 所得药物掺杂的纳米颗粒是球形的，高度单分散的，并且在水性体系中稳定。 用纳米颗粒内的光敏药物的合适波长的光照射导致产生单线态氧，其能够通过陶瓷基质的孔扩散出来。 本发明的载药陶瓷纳米颗粒可用作光动力疗法的药物载体。