公开(公告)号：US06514481B1

公开(公告)日：2003-02-04

申请号：US09718825

申请日：2000-11-22

申请人： Paras N. Prasad ,  Earl J. Bergey ,  Charles Liebow ,  Laurent Levy

发明人： Paras N. Prasad ,  Earl J. Bergey ,  Charles Liebow ,  Laurent Levy

IPC分类号： A61B5055

CPC分类号： B82Y5/00 ,  A61K47/64 ,  A61K47/6923 ,  A61N1/406 ,  A61N2/00 ,  Y10T436/24

摘要： The present invention provides novel nanosized particles termed as “nanoclinics” for therapeutic and/or diagnostic use. The particles have a core made of a therapeutic or diagnostic material surrounded by a shell. Further, the particles contain a targeting agent on the surface of the shell for specific recognition of targeted cells. A method is also provided for lysis of cells using DC magnetic field. Further, the present invention also provides a method for fabrication of nanoclinics that can target and lyse specific cells such as cancer cells.

摘要翻译： 本发明提供用于治疗和/或诊断用途的称为“纳米细胞学”的新型纳米尺寸颗粒。 颗粒具有由壳包围的治疗或诊断材料制成的芯。 此外，颗粒在壳的表面上含有靶向剂，用于特异性识别靶细胞。 还提供了使用DC磁场裂解细胞的方法。 此外，本发明还提供了可以靶向和裂解诸如癌细胞的特定细胞的纳米抗生素的制造方法。

公开(公告)号：US20110288234A1

公开(公告)日：2011-11-24

申请号：US12918232

申请日：2009-02-19

申请人： Ravindra K. Pandey ,  Lalit N. Goswami ,  Allan Oseroff ,  Stephanie Pincus ,  Janet Morgan ,  Paras N. Prasad ,  Earl J. Bergey

发明人： Ravindra K. Pandey ,  Lalit N. Goswami ,  Allan Oseroff ,  Stephanie Pincus ,  Janet Morgan ,  Paras N. Prasad ,  Earl J. Bergey

IPC分类号： C08G77/38 ,  C08F130/08 ,  C08G77/388 ,  B82Y5/00

CPC分类号： A61K49/0032 ,  A61K41/0071 ,  A61K49/0093 ,  A61K51/1255

摘要： A nanoparticle including a polysiloxane base having an exterior surface and having a photosensitizer at least partly exposed at its exterior surface, said photosensitizer being secured to the exterior surface by loading the photosensitizer onto the surface after formation of the polysiloxane base of the nanoparticle. The nanoparticle may have tumor targeting moieties and may be post loaded with cyanine dye. The nanoparticle preferably includes post loaded moieties providing at least two of tumor specificity, photodynamic properties and imaging capabilities and the photosensitizer is tagged with a radioisotope. A method for preparation of the nanoparticle is also provided.

摘要翻译： 包含具有外表面并具有至少部分地在其外表面暴露的光敏剂的聚硅氧烷基的纳米颗粒，所述光敏剂通过在形成纳米颗粒的聚硅氧烷基体之后将光敏剂加载到表面上而固定到外表面。 纳米颗粒可以具有肿瘤靶向部分，并且可以用花青染料后载。 纳米颗粒优选包括提供肿瘤特异性，光动力学特性和成像能力的至少两种的后加载部分，并且用放射性同位素标记光敏剂。 还提供了制备纳米颗粒的方法。

公开(公告)号：US20100125100A1

公开(公告)日：2010-05-20

申请号：US12555609

申请日：2009-09-08

申请人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

发明人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

IPC分类号： A61K31/7088 ,  A61P43/00

CPC分类号： C12N15/111 ,  A61K9/5115 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/32 ,  Y10S977/773

摘要： Provided are methods and compositions for inhibiting expression of one or more target genes. The compositions contain RNA polynucleotides that can inhibit expression of a target gene via RNA interference (RNAi) electrostatically complexed with surface functionalized gold nanorods (GNRs). The RNA polynucleotides are not covalently bound to the surface functionalized GNRs. The method involves inhibiting expression of a target gene in an individual. The method is performed by administering to the individual an effective amount of a composition containing surface functionalized GNRs electrostatically complexed with RNA polynucleotides, such as siRNA, that can inhibit expression of the target gene via RNAi. The siRNA is not covalently bound to the surface functionalized GNRs.

摘要翻译： 提供了用于抑制一种或多种靶基因表达的方法和组合物。 组合物含有可通过与表面官能化金纳米棒（GNR）静电复合的RNA干扰（RNAi）抑制靶基因表达的RNA多核苷酸。 RNA多核苷酸不共价结合表面官能化的GNRs。 该方法涉及抑制个体中靶基因的表达。 该方法通过向个体施用有效量的含有可以通过RNAi抑制靶基因表达的RNA多核苷酸（例如siRNA）静电复合的表面官能化GNR的组合物来进行。 siRNA不与表面官能化的GNR共价结合。

公开(公告)号：US08035016B2

公开(公告)日：2011-10-11

申请号：US12555609

申请日：2009-09-08

申请人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

发明人： Earl J. Bergey ,  Adela Bonoiu ,  Supriya Mahajan ,  Paras N. Prasad ,  Indrajit Roy ,  Stanley A. Schwartz ,  Ken-Tye Yong

IPC分类号： A61P43/00 ,  C12N15/113

CPC分类号： C12N15/111 ,  A61K9/5115 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/32 ,  Y10S977/773

摘要： Provided are methods and compositions for inhibiting expression of one or more target genes. The compositions contain RNA polynucleotides that can inhibit expression of a target gene via RNA interference (RNAi) electrostatically complexed with surface functionalized gold nanorods (GNRs). The RNA polynucleotides are not covalently bound to the surface functionalized GNRs. The method involves inhibiting expression of a target gene in an individual. The method is performed by administering to the individual an effective amount of a composition containing surface functionalized GNRs electrostatically complexed with RNA polynucleotides, such as siRNA, that can inhibit expression of the target gene via RNAi. The siRNA is not covalently bound to the surface functionalized GNRs.

摘要翻译： 提供了用于抑制一种或多种靶基因表达的方法和组合物。 组合物含有可通过与表面官能化金纳米棒（GNR）静电复合的RNA干扰（RNAi）抑制靶基因表达的RNA多核苷酸。 RNA多核苷酸不共价结合表面官能化的GNRs。 该方法涉及抑制个体中靶基因的表达。 该方法通过向个体施用有效量的含有可以通过RNAi抑制靶基因表达的RNA多核苷酸（例如siRNA）静电复合的表面官能化GNR的组合物来进行。 siRNA不与表面官能化的GNR共价结合。

公开(公告)号：US08004676B1

公开(公告)日：2011-08-23

申请号：US12069700

申请日：2008-02-11

申请人： Paras N. Prasad ,  Przemyslaw P. Markowicz ,  Wing Cheung Law ,  Andrei Kabashin ,  Sergiy Patskovsky

发明人： Paras N. Prasad ,  Przemyslaw P. Markowicz ,  Wing Cheung Law ,  Andrei Kabashin ,  Sergiy Patskovsky

IPC分类号： G01J4/00

CPC分类号： G01N21/553 ,  G01N2021/212

摘要： A method is provided for detection of analytes using the Surface Plasmon Resonance effect. The method comprises providing a metal film on a transparent substrate. The free surface of the metal film is exposed to a test sample. An anlyte in the sample can interact directly with the metal film or via analyte binding molecules (ABMs) complexed to the film. Light is directed incident to the surface of film in contact with the substrate. Light is reflected from the surface of the film under SPR conditions. The reflected light is collected and the second and/or third harmonics of the resulting electrical signal, which are indicative of the phase and polarization state of the reflected light, are determined. The second and third harmonics are correlated to the presence and/or concentration of the analyte.

摘要翻译： 提供了一种使用表面等离子体共振效应检测分析物的方法。 该方法包括在透明基底上提供金属膜。 将金属膜的自由表面暴露于测试样品。 样品中的非离子液体可以直接与金属膜或通过与膜复合的分析物结合分子（ABM）相互作用。 光引导入射到与基板接触的膜的表面。 在SPR条件下，光从膜的表面反射。 收集反射光，并且确定表示反射光的相位和极化状态的所得电信号的第二和/或第三谐波。 第二和第三谐波与分析物的存在和/或浓度相关。

公开(公告)号：US07413341B1

公开(公告)日：2008-08-19

申请号：US11204605

申请日：2005-08-16

申请人： Aliaksandr Kachynski ,  Andrey Kuzmin ,  Haridas Pudavar ,  Paras N. Prasad

发明人： Aliaksandr Kachynski ,  Andrey Kuzmin ,  Haridas Pudavar ,  Paras N. Prasad

IPC分类号： G01K11/00

CPC分类号： G01K11/20 ,  G01K2213/00

摘要： A method of local temperature measurement in a sample is provided. The method comprises the stimulation, at a location in the sample, of anti-Stokes and Stokes emission from fluorophores which are present at the location in the sample, measuring the intensities of the stimulated radiation and calculating the temperature at the location from the measured intensities. The method can be used to obtain a thermal image of the sample.

摘要翻译： 提供了样品中局部温度测量的方法。 该方法包括在样品中的位置处刺激存在于样品中的位置处的来自荧光团的反斯托克斯和斯托克斯发射，测量受激辐射的强度并根据测量的强度计算该位置处的温度 。 该方法可用于获得样品的热图像。

公开(公告)号：US06402037B1

公开(公告)日：2002-06-11

申请号：US09441720

申请日：1999-11-16

申请人： Paras N. Prasad ,  Jayant D. Bhawalker ,  Ping Chin Cheng ,  Shan Jen Pan

发明人： Paras N. Prasad ,  Jayant D. Bhawalker ,  Ping Chin Cheng ,  Shan Jen Pan

IPC分类号： G06K1900

CPC分类号： A61K49/0021 ,  A61K41/008 ,  A61K49/0084 ,  G11C13/04

摘要： The present invention is directed to styryl dyes having the formula: wherein D is an electron donating group; Q is an electron acceptor selected from the group consisting of electron acceptors having the formulae: W is an electron accepting group, R3 is selected from the group consisting of substituted or unsubstituted alkyl or substituted or unsubstituted aryl moieties, n is an integer from 0 to 4, A, B, and C are substituents of their rings and are each independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, sulfoalkyl, carboxyalkyl, and hydrogen, and Y is a counterion The present invention discloses media and methods for recording data. A three-dimensional matrix including a plurality of dye molecules, such a styryl dye molecule of the present invention, is provided. A first volume element in the matrix is exposed to actinic radiation for a duration and at an intensity effective to alter detectably a fraction between 0.3 and 0.7 of the dye molecules contained therein. The detectably altered dye molecules are substantially uniformly dispersed in the first volume element. The data storage methods and media of the present invention have approximately 1012 volume elements per square centimeter, and each of the volume elements can store a single bit, digital information of approximately 8 bits, or analog information. The data storage methods and media of the present invention are particularly useful for storing or archiving a series of two-dimensional black and white or color images, such as frames of a movie. Methods for reading data stored in the data storage media of the present invention using confocal microscopy are also disclosed.

摘要翻译： 本发明涉及具有下式的苯乙烯基染料：其中D是给电子基团; Q是选自下组的电子受体：具有下式的电子受体：W是电子接受基团，R 3选自 的取代或未取代的烷基或取代或未取代的芳基部分，n为0至4的整数，A，B和C为它们的环的取代基，并且各自独立地选自烷基，烷氧基，羟基烷基，磺基烷基， 羧基烷基和氢，Y是抗衡离子。本发明公开了用于记录数据的介质和方法。 提供了包含多个染料分子，如本发明的苯乙烯基染料分子的三维基质。 基质中的第一体积元素暴露于光化辐射持续时间，强度有效地改变其中所含的染料分子的0.3至0.7之间的分数。 可检测地改变的染料分子基本均匀地分散在第一容积元件中。 本发明的数据存储方法和介质具有每平方厘米大约1012体积的元素，并且每个体积元素可以存储大约8位的单位数字信息或模拟信息。 本发明的数据存储方法和媒体对于存储或归档一系列二维黑白图像或彩色图像（例如电影帧）特别有用。 还公开了使用共焦显微镜读取存储在本发明的数据存储介质中的数据的方法。

公开(公告)号：US20090035576A1

公开(公告)日：2009-02-05

申请号：US11900334

申请日：2007-09-10

申请人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

发明人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

IPC分类号： B32B5/16

CPC分类号： A61K41/0071 ,  A61K41/008 ,  A61K49/0021 ,  A61K49/0093 ,  C01B33/141 ,  G01N33/582 ,  G01N33/585 ,  Y10T428/2982

摘要： The present invention provides organically modified silica (ORMOSIL) nanoparticles into which have been incorporated two-photon absorption dye molecules. The two photon absorption dye displays a unique aggregation induced fluorescence enhancement behavior. As a result ORMOSIL nanoparticles with high amounts of the dye can be prepared. These particles can be used for imaging. In one embodiment, the nanoparticles can additionally have incorporated therein a photosensitizer. The photosensitzer can be activated by intraparticle fluorescence resonance energy transfer (FRET) from the dye aggregates resulting in enhanced fluorescence and singlet oxygen generation from photosensitizer under two-photon excitation conditions. Such nanoparticles can be used for photodynamic therapy applications.

摘要翻译： 本发明提供了已经掺入双光子吸收染料分子的有机改性二氧化硅（ORMOSIL）纳米颗粒。 两个光子吸收染料显示出独特的聚集诱导的荧光增强行为。 因此，可以制备具有大量染料的ORMOSIL纳米颗粒。 这些颗粒可用于成像。 在一个实施方案中，纳米颗粒另外可以掺入光敏剂。 光敏剂可以通过来自染料聚集体的颗粒内荧光共振能量转移（FRET）来激活，从而在双光子激发条件下从光敏剂产生增强的荧光和单线态氧产生。 这种纳米颗粒可用于光动力疗法应用。

公开(公告)号：US07449578B1

公开(公告)日：2008-11-11

申请号：US11087236

申请日：2005-03-23

申请人： Qingdong Zheng ,  Paras N. Prasad ,  Guang S. He

发明人： Qingdong Zheng ,  Paras N. Prasad ,  Guang S. He

IPC分类号： C07F15/04 ,  C07D471/02

CPC分类号： C07D471/04 ,  B82Y10/00 ,  G11B2007/24624

摘要： The present invention provides substituted phenanthroline compounds with high two photon absorption cross sections, as well as substituted phenanthroline compounds which additionally have quenched fluorescence emission upon two photon absorption.

摘要翻译： 本发明提供了具有高双光子吸收横截面的取代菲咯啉化合物，以及在两光子吸收时另外具有骤冷荧光发射的取代菲咯啉化合物。

公开(公告)号：US20080233051A1

公开(公告)日：2008-09-25

申请号：US11983845

申请日：2007-11-13

申请人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

发明人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

IPC分类号： A61K9/14 ,  A61K31/695 ,  A61K49/00

CPC分类号： A61K31/695 ,  A61K41/0071 ,  A61K41/008 ,  A61K49/0021 ,  A61K49/0093

摘要： The present invention provides organically modified silica (ORMOSIL) nanoparticles into which have been incorporated two-photon absorption dye molecules. The two photon absorption dye displays a unique aggregation induced fluorescence enhancement behavior. As a result ORMOSIL nanoparticles with high amounts of the dye can be prepared. These particles can be used for imaging. In one embodiment, the nanoparticles can additionally have incorporated therein a photosensitizer. The photosensitizer can be activated by intraparticle fluorescence resonance energy transfer (FRET) from the dye aggregates resulting in enhanced fluorescence and singlet oxygen generation from photosensitizer under two-photon excitation conditions. Such nanoparticles can be used for photodynamic therapy applications.

摘要翻译： 本发明提供了已经掺入双光子吸收染料分子的有机改性二氧化硅（ORMOSIL）纳米颗粒。 两个光子吸收染料显示出独特的聚集诱导的荧光增强行为。 因此，可以制备具有大量染料的ORMOSIL纳米颗粒。 这些颗粒可用于成像。 在一个实施方案中，纳米颗粒另外可以掺入光敏剂。 可以通过来自染料聚集体的颗粒内荧光共振能量转移（FRET）来激活光敏剂，从而在双光子激发条件下从光敏剂产生增强的荧光和单线态氧产生。 这种纳米颗粒可用于光动力疗法应用。