公开(公告)号：US10953404B1

公开(公告)日：2021-03-23

申请号：US16858282

申请日：2020-04-24

申请人： Pattern Bioscience, Inc.

发明人： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC分类号： B01L3/00 ,  B01L9/00 ,  B01L1/02 ,  G01N21/31

摘要： An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US20210331178A1

公开(公告)日：2021-10-28

申请号：US17195206

申请日：2021-03-08

申请人： Pattern Bioscience, Inc.

发明人： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC分类号： B01L9/00 ,  B01L1/02 ,  G01N21/31 ,  B01L3/00

摘要： An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US12128415B2

公开(公告)日：2024-10-29

申请号：US17195206

申请日：2021-03-08

申请人： Pattern Bioscience, Inc.

发明人： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC分类号： B01L9/00 ,  B01L1/02 ,  B01L3/00 ,  G01N21/31

CPC分类号： B01L9/527 ,  B01L1/02 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  G01N21/31 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0663 ,  B01L2400/049

摘要： An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US11344890B2

公开(公告)日：2022-05-31

申请号：US16661829

申请日：2019-10-23

申请人： Pattern Bioscience, Inc.

发明人： Nicolas Arab ,  Ross Johnson ,  David Bussian ,  Jon Isom

IPC分类号： B01L3/00 ,  G01N35/08

摘要： A microfluidic chip that can have a body defining a microfluidic network including a test volume, one or more ports, and one or more channels in fluid communication between the port(s) and the test volume. Gas can be removed from the test volume before a sample liquid is introduced therein by reducing pressure at a first one of the port(s), optionally while the liquid is disposed in the port. Liquid in the first port can be introduced into the test volume by increasing pressure at the first port. The microfluidic network can define one or more droplet-generating regions in which at least one of the channel(s) defines a constriction and/or two or more of the channels connect at a junction. Liquid flowing from the first port can pass through at least one of the droplet-generating region(s) and to the test volume.