公开(公告)号：US5591823A

公开(公告)日：1997-01-07

申请号：US169813

申请日：1993-12-17

申请人： Paul P. Hung ,  Shaw-Guang L. Lee ,  Narender K. Kalyan

发明人： Paul P. Hung ,  Shaw-Guang L. Lee ,  Narender K. Kalyan

IPC分类号： A61K39/21 ,  A61K39/00 ,  A61P31/12 ,  A61P31/16 ,  A61P37/08 ,  C07H21/00 ,  C07K14/11 ,  C07K14/16 ,  C12N7/01 ,  C12N15/08 ,  C12N15/09 ,  C12N15/11 ,  C12N15/32 ,  C12N15/34 ,  C12N15/44 ,  C12P21/02 ,  G01N33/53 ,  G01N33/569 ,  C07K1/00 ,  A61K39/145 ,  C12Q1/70

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2319/40 ,  C12N2740/16022 ,  C12N2760/16122

摘要： Chimeric DNA fragments are provided which include a nucleotide sequence substantially the same as that which codes for the HA surface protein of an influenza A virus having five immunodominant antigenic sites, wherein a nucleotide sequence substantially the same as that which codes for a foreign epitope is inserted into the nucleotide sequence of an antigenic site. Corresponding chimeric peptides, expression vectors, and transformed hosts are provided as well. These peptides are useful in providing vaccines against the respective antigens and in test kits to detect the exposure to such antigens. Additionally, these peptides or their corresponding antibodies are useful in methods of treatment and prevention of the manifestations of exposure to these antigens, including immunotherapy.

摘要翻译： 提供了嵌合DNA片段，其包括与编码具有5个免疫显性抗原位点的甲型流感病毒的HA表面蛋白基本上相同的核苷酸序列，其中插入与编码外源表位的碱基序列基本相同的核苷酸序列 进入抗原位点的核苷酸序列。 也提供相应的嵌合肽，表达载体和转化的宿主。 这些肽可用于提供针对相应抗原和测试试剂盒的疫苗以检测对这些抗原的暴露。 此外，这些肽或其相应的抗体可用于治疗和预防暴露于这些抗原的表现的方法，包括免疫治疗。

公开(公告)号：US06511845B1

公开(公告)日：2003-01-28

申请号：US09618360

申请日：2000-07-18

申请人： Alan R. Davis ,  Paul P. Hung ,  Michael D. Lubeck ,  Robert J. Natuk ,  Pranab K. Chanda ,  Shridhara C. S. Murthy ,  Shaw-Guang L. Lee

发明人： Alan R. Davis ,  Paul P. Hung ,  Michael D. Lubeck ,  Robert J. Natuk ,  Pranab K. Chanda ,  Shridhara C. S. Murthy ,  Shaw-Guang L. Lee

IPC分类号： A61K3921

CPC分类号： C12N15/86 ,  A61K39/00 ,  C07K14/005 ,  C12N2710/10343 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  Y02A50/464 ,  Y02A50/472

摘要： This invention provides a method of protecting a primate from an infectious organism by stimulating the production of antibodies or cell mediated immunity to the infectious organism which comprises administering to said primate intranasally, intramuscularly, or subcutaneously, live recombinant adenoviruses in which the virion structural protein is unchanged from that in the native adenovirus from which the recombinant adenovirus is produced, and which contain the gene coding for the antigen corresponding to said antibodies or inducing said cell mediated immunity. Preferably, the infectious organism is HIV and the primate is a human.

摘要翻译： 本发明提供了通过刺激产生抗体或细胞介导的对感染性生物的免疫来保护灵长类从感染性生物体的方法，其包括向所述灵长类动物鼻内，肌内或皮下施用病毒结构蛋白质的活重组腺病毒 与从其产生重组腺病毒的天然腺病毒中不同，并且其含有编码对应于所述抗体的抗原的基因或诱导所述细胞介导的免疫。 优选地，感染性生物是HIV，灵长类是人。