利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.02 秒)

    Inhibition of protease activity of human whole blood cell lysates
    2.
    发明授权
    Inhibition of protease activity of human whole blood cell lysates 失效
    抑制人全血细胞裂解物的蛋白酶活性

    公开(公告)号：US5863742A

    公开(公告)日：1999-01-26

    申请号：US212442

    申请日：1994-03-10

    申请人： Se-Kyung Oh ,  Harry Towbin

    发明人： Se-Kyung Oh ,  Harry Towbin

    IPC分类号： G01N33/48 ,  C12N9/99 ,  C12Q1/37 ,  G01N1/28 ,  G01N33/53 ,  G01N33/577 ,  G01N33/68 ,  G01N33/567

    CPC分类号： C12Q1/37 ,  G01N33/6866 ,  Y10S435/962 ,  Y10S435/967

    摘要： A method of inhibiting proteolytic degradation of a thermally-stable intracellular protein is described. The method involves adding 1 or more denaturing agents to a sample which contains the protease and the protein of interest and heating the resulting solution at a temperature and for period of time sufficient to denature the protease. The method optionally includes a step for lysing the cell if the protein of interest is contained in a cell in order to release said protein. Additionally, a method of determining Mx protein induced by interferon in a blood sample is described. The method involves adding to a blood sample a lysing agent, a denaturing agent, and a detergent selected to solubilize Mx protein. The sample containing Mx protein is then heated at a temperature of from about 50.degree. C. to about 60.degree. C. for a period of time of from about 1 minute to about 30 minutes, and the Mx protein in the solution then is determined. Also described is a solution including a synthetic matrix mimicking whole blood cell lysates, or actual whole blood cell lysates, to which a known concentration of a thermally-stable intracellular protein is added to prepare a control material. In addition, a solution free of a protease that degrades the intracellular protein is disclosed, such solution remaining stable at 4.degree. C. for at least three weeks.

    摘要翻译： 描述了抑制热稳定细胞内蛋白质的蛋白水解降解的方法。 该方法包括向含有蛋白酶和目标蛋白质的样品中加入1种或更多种变性剂，并在足以使蛋白酶变性的温度和时间内加热所得溶液。 如果感兴趣的蛋白质包含在细胞中以释放所述蛋白质，该方法可选地包括用于裂解细胞的步骤。 另外，描述了确定血液样品中由干扰素诱导的Mx蛋白的方法。 该方法包括向血液样品中加入裂解剂，变性剂和选择用于溶解Mx蛋白质的洗涤剂。 然后将含有Mx蛋白的样品在约50℃至约60℃的温度下加热约1分钟至约30分钟的时间，然后测定溶液中的Mx蛋白。 还描述了一种溶液，其包括模拟全血细胞裂解物或实际全血细胞裂解物的合成基质，向其中加入已知浓度的热稳定细胞内蛋白质以制备对照物质。 此外，公开了一种不含降解细胞内蛋白质的蛋白酶的溶液，这种溶液在4℃下保持稳定至少三周。

    Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays
    3.
    发明授权
    Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays 失效
    将电泳图电泳转移到硝酸纤维素片上用于免疫测定

    公开(公告)号：US4452901A

    公开(公告)日：1984-06-05

    申请号：US261217

    申请日：1981-04-20

    申请人： Julian Gordon ,  Theophil Staehelin ,  Harry Towbin

    发明人： Julian Gordon ,  Theophil Staehelin ,  Harry Towbin

    IPC分类号： G01N27/447 ,  G01N33/558 ,  G01N33/54 ,  G01N27/26 ,  G01N33/68

    CPC分类号： G01N27/4473 ,  G01N33/558 ,  Y10S435/962

    摘要： Solid supports for proteins consisting of nitrocellulose sheets containing a replica of an electropherogram of proteins as obtained by electrophoretic separation in a gel. Faithful replica of such electropherograms on the nitrocellulose support can be obtained by contacting the gel with a nitrocellulose sheet and applying an electric field perpendicular to the plane of the gel causing an electrophoretic migration of the proteins toward the nitrocellulose sheet where the proteins are adsorbed. Various analytical problems and especially immuno-assays, for enzyme immuno-assays or such involving radioactively labeled indicators, can be made with the new solid supports.

    摘要翻译： PCT No.PCT / EP80 / 00018 Sec。 371日期1981年4月20日 102（e）日期1981年4月20日PCT提交1980年3月18日PCT公布。 公开号WO81 / 02790 日期：1981年10月1日。由含有通过凝胶电泳分离获得的蛋白质电泳图复制品的硝酸纤维素片组成的蛋白质的固体支持物。 可以通过使凝胶与硝酸纤维素片接触并施加垂直于凝胶平面的电场，使蛋白质电泳迁移到蛋白质被吸附的硝酸纤维素片上，可以获得硝酸纤维素载体上的这种电泳图的忠实复制品。 可以使用新的固体支持物进行各种分析问题，特别是用于酶免疫测定或涉及放射性标记的指示剂的免疫测定。