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公开(公告)号：US5650307A
公开(公告)日：1997-07-22
申请号：US469856
申请日：1995-06-06
申请人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
发明人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
IPC分类号： C12N15/09 , C07K14/415 , C07K14/765 , C12N5/10 , C12N15/82 , C12P21/02 , C12N15/63 , A01H5/00
CPC分类号： C12N15/8221 , C07K14/415 , C07K14/765 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C07K2319/02
摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.
摘要翻译： 本发明提供了在植物宿主中产生排泄且正确处理的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。
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公开(公告)号：US5763748A
公开(公告)日：1998-06-09
申请号：US829057
申请日：1997-03-31
申请人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
发明人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
IPC分类号： C12N15/09 , C07K14/415 , C07K14/765 , C12N5/10 , C12N15/82 , C12P21/02 , C12N5/00 , A01H5/00 , C12N15/14
CPC分类号： C12N15/8221 , C07K14/415 , C07K14/765 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C07K2319/02
摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.
摘要翻译： 本发明提供了在植物宿主中产生排泄且正确处理的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。
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公开(公告)号：US5716802A
公开(公告)日：1998-02-10
申请号：US659287
申请日：1991-05-21
申请人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
发明人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
IPC分类号： C12N15/09 , C07K14/415 , C07K14/765 , C12N5/10 , C12N15/82 , C12P21/02 , A01H1/00 , A01H5/00 , C12N15/14
CPC分类号： C12N15/8221 , C07K14/415 , C07K14/765 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C07K2319/02
摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.
摘要翻译： PCT No.PCT / NL90 / 00108 Sec。 371日期1991年3月21日 102（e）1991年3月21日PCT文件1990年7月26日PCT公布。 WO91 / 02066 PCT出版物 日期1991年2月21日本发明提供了在植物宿主中产生排泄物和正确加工的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。
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公开(公告)号：US07314737B2
公开(公告)日：2008-01-01
申请号：US10300666
申请日：2002-11-19
CPC分类号： C12N15/62 , C07K14/195 , C07K2319/00 , C12N9/00 , C12N15/8205
摘要： A method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention, a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A, an oligopeptide comprising the C-terminal amino acids 1-20 of VirF, VirD2, VirE2, VirE3, VirD5 or MobA, or an analogue thereof and ii) as a second part B, a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof linked with the N-terminal end of the first part.
摘要翻译： 使用与要改变的单元接触的传送系统来实现单元的改变的方法。 转移系统包含融合蛋白，其使用VirB / VirD4转移系统转移到细胞中。 根据本发明，将融合蛋白BA引入要改变的细胞中，其包含i）作为第一部分A，包含VirF，VirD2，VirE2，VirE3，VirD5或MobA的C末端氨基酸1-20的寡肽 ，或其类似物，和ii）作为第二部分B，能够在待改变的细胞中实现细胞变化活性的多肽，其中所述多肽与其N-末端连接的C末端连接 的第一部分。
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