公开(公告)号：US5650307A

公开(公告)日：1997-07-22

申请号：US469856

申请日：1995-06-06

申请人： Peter Christiaan Sijmons ,  Andreas Hoekema ,  Bernardus Martinus M. Dekker ,  Barbara Schrammeijer ,  Teunis Cornelius Verwoerd ,  Peturs Josephus M. Van Den Elzen

发明人： Peter Christiaan Sijmons ,  Andreas Hoekema ,  Bernardus Martinus M. Dekker ,  Barbara Schrammeijer ,  Teunis Cornelius Verwoerd ,  Peturs Josephus M. Van Den Elzen

IPC分类号： C12N15/09 ,  C07K14/415 ,  C07K14/765 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12N15/63 ,  A01H5/00

CPC分类号： C12N15/8221 ,  C07K14/415 ,  C07K14/765 ,  C12N15/8216 ,  C12N15/8242 ,  C12N15/8257 ,  C07K2319/02

摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.

摘要翻译： 本发明提供了在植物宿主中产生排泄且正确处理的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。

公开(公告)号：US5763748A

公开(公告)日：1998-06-09

申请号：US829057

申请日：1997-03-31

申请人： Peter Christiaan Sijmons ,  Andreas Hoekema ,  Bernardus Martinus M. Dekker ,  Barbara Schrammeijer ,  Teunis Cornelius Verwoerd ,  Peturs Josephus M. Van Den Elzen

发明人： Peter Christiaan Sijmons ,  Andreas Hoekema ,  Bernardus Martinus M. Dekker ,  Barbara Schrammeijer ,  Teunis Cornelius Verwoerd ,  Peturs Josephus M. Van Den Elzen

IPC分类号： C12N15/09 ,  C07K14/415 ,  C07K14/765 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12N5/00 ,  A01H5/00 ,  C12N15/14

CPC分类号： C12N15/8221 ,  C07K14/415 ,  C07K14/765 ,  C12N15/8216 ,  C12N15/8242 ,  C12N15/8257 ,  C07K2319/02

摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.

摘要翻译： 本发明提供了在植物宿主中产生排泄且正确处理的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。

公开(公告)号：US5716802A

公开(公告)日：1998-02-10

申请号：US659287

申请日：1991-05-21

申请人： Peter Christiaan Sijmons ,  Andreas Hoekema ,  Bernardus Martinus M. Dekker ,  Barbara Schrammeijer ,  Teunis Cornelius Verwoerd ,  Peturs Josephus M. Van Den Elzen

发明人： Peter Christiaan Sijmons ,  Andreas Hoekema ,  Bernardus Martinus M. Dekker ,  Barbara Schrammeijer ,  Teunis Cornelius Verwoerd ,  Peturs Josephus M. Van Den Elzen

IPC分类号： C12N15/09 ,  C07K14/415 ,  C07K14/765 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  A01H1/00 ,  A01H5/00 ,  C12N15/14

CPC分类号： C12N15/8221 ,  C07K14/415 ,  C07K14/765 ,  C12N15/8216 ,  C12N15/8242 ,  C12N15/8257 ,  C07K2319/02

摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.

摘要翻译： PCT No.PCT / NL90 / 00108 Sec。 371日期1991年3月21日 102（e）1991年3月21日PCT文件1990年7月26日PCT公布。 WO91 / 02066 PCT出版物 日期1991年2月21日本发明提供了在植物宿主中产生排泄物和正确加工的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。

公开(公告)号：US5705375A

公开(公告)日：1998-01-06

申请号：US253575

申请日：1994-06-03

申请人： Albert Johannes Joseph Van Ooyen ,  Krijn Rietveld ,  Wilhelmus Johannes Quax ,  Petrus Josephus Maria Van Den Elzen ,  Jan Pen ,  Andreas Hoekema ,  Peter Christiaan Sijmons

发明人： Albert Johannes Joseph Van Ooyen ,  Krijn Rietveld ,  Wilhelmus Johannes Quax ,  Petrus Josephus Maria Van Den Elzen ,  Jan Pen ,  Andreas Hoekema ,  Peter Christiaan Sijmons

IPC分类号： C12N1/21 ,  C12N15/82 ,  A01H5/00 ,  C12N15/56

CPC分类号： C12N15/8245

摘要： The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.

公开(公告)号：US07033627B2

公开(公告)日：2006-04-25

申请号：US09149310

申请日：1998-02-02

申请人： Albert Johannes Joseph Van Ooyen ,  Krijn Rietveld ,  Wilhelmus Johannes Quax ,  Jan Pen ,  Andreas Hoekema ,  Peter Christiaan Sijmons ,  Teunis Cornelis Verwoerd

发明人： Albert Johannes Joseph Van Ooyen ,  Krijn Rietveld ,  Wilhelmus Johannes Quax ,  Jan Pen ,  Andreas Hoekema ,  Peter Christiaan Sijmons ,  Teunis Cornelis Verwoerd

IPC分类号： A21D8/04 ,  A21D2/00

CPC分类号： C12Y301/03026 ,  A21D8/042 ,  A23K20/189 ,  A23L29/06 ,  C12N9/16 ,  C12N9/2417 ,  C12N15/8242 ,  C12N15/8243 ,  C12N15/8257

摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将转基因非野生型种子，优选以研磨形式加入到反应混合物，特别是面包店方法中，并允许种子中的酶提高反应速率。 通过将种子直接加入到反应混合物中，该方法提供了提取和纯化酶的昂贵且有问题的方法的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。

公开(公告)号：US5593963A

公开(公告)日：1997-01-14

申请号：US146424

申请日：1993-11-02

申请人： Albert J. J. Van Ooijen ,  Krijn Rietveld ,  Andreas Hoekema ,  Jan Pen ,  Peter C. Sijmons ,  Teunis C. Verwoerd

发明人： Albert J. J. Van Ooijen ,  Krijn Rietveld ,  Andreas Hoekema ,  Jan Pen ,  Peter C. Sijmons ,  Teunis C. Verwoerd

IPC分类号： A61K38/46 ,  C12N15/83 ,  A01H5/00 ,  A23K1/165

CPC分类号： A61K38/465 ,  A23K10/10 ,  A23K10/30 ,  A23K20/189 ,  C12N15/8242 ,  C12N9/16 ,  C12Y301/03026

摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

摘要翻译： 本发明提供了植酸酶在转基因植物或植物器官中的表达及其生产方法。 提供DNA表达构建体用于在能够指导植酸酶表达的调控序列的控制下用编码植酸酶的基因转化植物。 这些调节序列包括根据植物或其部分的用途能够在植物中指导转录的序列，组成型或阶段和/或组织特异性的序列。 本发明提供的转基因植物和植物器官可以直接应用于各种工业过程，例如， 在动物饲料中，或者，可以提取表达的肌醇六磷酸酶，如果需要，在施用前进行纯化。

公开(公告)号：US4940838A

公开(公告)日：1990-07-10

申请号：US583022

申请日：1984-02-23

申请人： Robbert A. Schilperoort ,  Andreas Hoekema ,  Paul J. J. Hooykaas

发明人： Robbert A. Schilperoort ,  Andreas Hoekema ,  Paul J. J. Hooykaas

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N1/20 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/82 ,  C12R1/01

CPC分类号： C12N15/8205

摘要： The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of a Ti (tumour inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria wherein at least one plasmid which has the vir-region of a Ti (tumour inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.

摘要翻译： 本发明涉及通过用含有一种或多种质粒的农杆菌细菌感染植物或培养植物原生质体将外来DNA掺入双子叶植物的染色体的方法，其中使用含有至少一种具有vir- Ti（肿瘤诱导）质粒但不含T区的区域，以及至少一种具有T区的外源DNA但不含有vir区的其他质粒，以及至​​少一种质粒， 具有Ti（肿瘤诱导）质粒的vir区域，但没有T区和至少一个具有野生型T区的其他质粒，其中掺入外源DNA但没有vir区。

公开(公告)号：US20060195940A1

公开(公告)日：2006-08-31

申请号：US11193968

申请日：2005-07-30

申请人： Albert Van Ooyen ,  Krijn Rietveld ,  Wilhelmus Quax ,  Petrus Van Den Elzen ,  Jan Pen ,  Andreas Hoekema ,  Peter Sijmons

发明人： Albert Van Ooyen ,  Krijn Rietveld ,  Wilhelmus Quax ,  Petrus Van Den Elzen ,  Jan Pen ,  Andreas Hoekema ,  Peter Sijmons

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/2411 ,  A01H1/00 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/242 ,  C12N9/2428 ,  C12N15/8245

摘要： The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.

摘要翻译： 本发明为植物提供改良的味道，固体含量和/或质地。 本发明还提供了通过使用含有能够降解植物多糖的酶的基因的DNA构建体和任选地能够进一步修饰由第一降解步骤产生的降解产物的另外的编码酶的基因进行转化来获得这样的植物的方法。

公开(公告)号：US5770413A

公开(公告)日：1998-06-23

申请号：US693709

申请日：1996-08-07

申请人： Albert J. J. Van Ooijen ,  Krijn Rietveld ,  Andreas Hoekema ,  Jan Pen ,  Peter Christian Sijmons ,  Teunis Cornelis Verwoerd

发明人： Albert J. J. Van Ooijen ,  Krijn Rietveld ,  Andreas Hoekema ,  Jan Pen ,  Peter Christian Sijmons ,  Teunis Cornelis Verwoerd

IPC分类号： A01H1/00 ,  A01H5/00 ,  A23K1/14 ,  A23K1/16 ,  A23K1/165 ,  C12N1/21 ,  C12N5/10 ,  C12N9/16 ,  C12N9/28 ,  C12N15/00 ,  C12N15/09 ,  C12N15/55 ,  C12N15/82 ,  C12P3/00 ,  C12P7/02 ,  C12P7/18 ,  C12R1/01 ,  C12R1/66

CPC分类号： C12Y301/03026 ,  A23K20/189 ,  C12N15/8242 ,  C12N15/8243 ,  C12N15/8257 ,  C12N9/16 ,  C12N9/2417

摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

公开(公告)号：US5464763A

公开(公告)日：1995-11-07

申请号：US173271

申请日：1993-12-23

申请人： Robbert A. Schilperoort ,  Andreas Hoekema

发明人： Robbert A. Schilperoort ,  Andreas Hoekema

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N1/20 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/82 ,  C12R1/01 ,  C12N15/84 ,  C12N15/74

CPC分类号： C12N15/8205

摘要： The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of Ti (tumor inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria, suitable for use in the process according to claim 1 wherein at least one plasmid which has the vir-region of a Ti (tumor inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.

摘要翻译： 本发明涉及通过用含有一种或多种质粒的农杆菌细菌感染植物或培养植物原生质体将外来DNA掺入双子叶植物的染色体的方法，其中使用含有至少一种具有vir- Ti（肿瘤诱导）质粒的区域，但没有T区，以及至少一种其他具有T区的外源DNA但不含有vir区的质粒，以及适用于该方法的农杆菌 根据权利要求1的方法，其中至少一种具有Ti（诱导肿瘤）质粒但不具有T区的vir区的至少一种质粒和至少一种具有野生型T区的其它质粒，其中掺入外源DNA但不含 vir区域。