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    Site specific incorporation of keto amino acids into proteins
    1.
    发明授权
    Site specific incorporation of keto amino acids into proteins 有权
    现场特异性地将酮基氨基酸并入蛋白质

    公开(公告)号：US08071344B2

    公开(公告)日：2011-12-06

    申请号：US11978182

    申请日：2007-10-26

    申请人： Peter G. Schultz ,  Lei Wang

    发明人： Peter G. Schultz ,  Lei Wang

    IPC分类号： C12P21/04 ,  C12N9/00

    CPC分类号： C12N15/67 ,  C12N9/93 ,  C12N15/10 ,  C12P21/005

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.

    摘要翻译： 提供了将酮基氨基酸并入蛋白质中的正交tRNA，正交氨基-tRNA合成酶和正交对tRNA /合成酶的蛋白质生物合成机理的组成和方法。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生具有酮基氨基酸的蛋白质的方法。

    Glycoprotein synthesis
    3.
    发明申请
    Glycoprotein synthesis 失效
    糖蛋白合成

    公开(公告)号：US20080213828A1

    公开(公告)日：2008-09-04

    申请号：US11978232

    申请日：2007-10-26

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： C07K14/435 ,  C12P21/00

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    摘要翻译： 提供在体外和体内制备糖蛋白的方法。 一种方法包括将非天然氨基酸掺入蛋白质并将一个或多个糖部分连接到非天然氨基酸上。 另一种方法包括将包含糖部分的非天然氨基酸掺入蛋白质中。 通过两种方法制备的蛋白质可以用额外的糖进一步修饰。

    Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
    4.
    发明授权
    Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination 失效
    将重原子含有非天然氨基酸的位点特异性并入蛋白质进行结构测定

    公开(公告)号：US07399619B2

    公开(公告)日：2008-07-15

    申请号：US11137850

    申请日：2005-05-24

    申请人： Jianming Xie ,  Lei Wang ,  Ning Wu ,  Peter G. Schultz

    发明人： Jianming Xie ,  Lei Wang ,  Ning Wu ,  Peter G. Schultz

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/00 ,  C12N1/21 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

    CPC分类号： C12N9/93 ,  C07H21/04 ,  C12N15/67 ,  C12P21/02

    摘要： Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

    摘要翻译： 提供了翻译系统和包括正交氨酰基tRNA合成酶的其它组合物，其优先用碘化或溴化氨基酸对正交的tRNA进行充电。 还描述了编码这种合成酶的核酸，以及用于产生蛋白质的方法和试剂盒，包括含重原子的氨基酸，例如溴化或碘化​​氨基酸。 还描述了确定蛋白质结构的方法，例如通过使用正交的tRNA /氨基酰基tRNA-合成酶对将重原子特异性并入的蛋白质的蛋白质。

    Glycoprotein synthesis
    5.
    发明授权
    Glycoprotein synthesis 失效

    公开(公告)号：US07183082B2

    公开(公告)日：2007-02-27

    申请号：US11093798

    申请日：2005-03-29

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： C12P19/00

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    Glycoprotein synthesis
    7.
    发明申请
    Glycoprotein synthesis 审中-公开

    公开(公告)号：US20100009425A1

    公开(公告)日：2010-01-14

    申请号：US12454390

    申请日：2009-05-15

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： C12N9/24

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    Site specific incorporation of keto amino acids into proteins
    8.
    发明授权
    Site specific incorporation of keto amino acids into proteins 有权
    现场特异性地将酮基氨基酸并入蛋白质

    公开(公告)号：US07524647B2

    公开(公告)日：2009-04-28

    申请号：US11978160

    申请日：2007-10-26

    申请人： Peter G. Schultz ,  Lei Wang

    发明人： Peter G. Schultz ,  Lei Wang

    IPC分类号： C12P21/06 ,  C12N9/22 ,  C12N15/87

    CPC分类号： C12N15/67 ,  C12N9/93 ,  C12N15/10 ,  C12P21/005

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.

    摘要翻译： 提供了将酮基氨基酸并入蛋白质中的正交tRNA，正交氨基-tRNA合成酶和正交对tRNA /合成酶的蛋白质生物合成机理的组成和方法。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生具有酮基氨基酸的蛋白质的方法。