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公开(公告)号：US09441239B2
公开(公告)日：2016-09-13
申请号：US11364183
申请日：2006-03-01
CPC分类号： C12N15/8218 , A01K2217/05 , A61K48/00 , C12N15/8203 , C12N15/8247 , C12N15/8282 , C12N15/8283
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.
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公开(公告)号：US08598332B1
公开(公告)日：2013-12-03
申请号：US09287632
申请日：1999-04-07
CPC分类号： C12N15/8218 , C12N15/8247
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.
摘要翻译： 提供了通过引入编码针对靶核酸的正义和反义RNA分子的嵌合基因来降低真核细胞，特别是植物细胞中感兴趣的核酸的表型表达的方法和手段，其能够形成双链 RNA区域通过有义和反义核苷酸序列的区域之间的碱基配对或通过引入RNA分子本身。 优选地，RNA分子同时包含有义和反义核苷酸序列。
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公开(公告)号：US07138565B2
公开(公告)日：2006-11-21
申请号：US10152808
申请日：2002-05-23
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
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公开(公告)号：US08334374B2
公开(公告)日：2012-12-18
申请号：US13474539
申请日：2012-05-17
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞，特别是植物细胞中目的核酸的表型表达的方法和手段。 优选地，未多聚腺苷酸化的靶特异性RNA通过包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'端形成和多聚腺苷酸化的DNA区的嵌合基因的转录来提供。
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公开(公告)号：US20120210469A1
公开(公告)日：2012-08-16
申请号：US13092645
申请日：2011-04-22
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞，特别是植物细胞中目的核酸的表型表达的方法和手段。 优选地，未多聚腺苷酸化的靶特异性RNA通过包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'端形成和多聚腺苷酸化的DNA区的嵌合基因的转录来提供。
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公开(公告)号：US06423885B1
公开(公告)日：2002-07-23
申请号：US09373720
申请日：1999-08-13
IPC分类号： C07H2104
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods are provided for reducing the phenotypic expression of a nucleic acid of interest in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞的细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来降低植物细胞中目的核酸的表型表达的方法。 优选地，未多聚腺苷酸化的靶特异性RNA通过包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'端形成和多聚腺苷酸化的DNA区的嵌合基因的转录来提供。
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公开(公告)号：US09399777B2
公开(公告)日：2016-07-26
申请号：US13092645
申请日：2011-04-22
IPC分类号： C12N15/113 , C12N15/82
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞，特别是植物细胞中目的核酸的表型表达的方法和手段。 优选地，未多聚腺苷酸化的靶特异性RNA通过包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'端形成和多聚腺苷酸化的DNA区的嵌合基因的转录来提供。
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公开(公告)号：US20120270315A1
公开(公告)日：2012-10-25
申请号：US13474539
申请日：2012-05-17
IPC分类号： C12N15/113 , C12N5/10
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specifc RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞，特别是植物细胞中目的核酸的表型表达的方法和手段。 优选地，通过转录包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'末端形成和多聚腺苷酸化的DNA区的嵌合基因来提供未多聚腺苷酸化的靶特异性RNA。
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公开(公告)号：US20080044906A1
公开(公告)日：2008-02-21
申请号：US10571384
申请日：2004-09-10
申请人： Peter Michael Waterhouse , Linda Jane Lockett , Ming-Bo Wang , Timothy James Doran , Robert John Moore , Gerald Wayne Both
发明人： Peter Michael Waterhouse , Linda Jane Lockett , Ming-Bo Wang , Timothy James Doran , Robert John Moore , Gerald Wayne Both
CPC分类号： C12N15/113 , A01K2217/05 , A01K2217/058 , C12N15/11 , C12N15/1131 , C12N2310/11 , C12N2310/111 , C12N2310/3519 , C12N2310/53 , C12N2799/027
摘要： Methods and means for efficiently downregulating the expression of a target gene of interest in cell from an organism that is an animal, fungus and protist. The invention provides chimeric nucleic acid molecules for downregulating target genes. The invention also provides modified cells and organisms comprising the chimeric nucleic acid molecules and compositions comprising the chimeric molecules.
摘要翻译： 用于从动物，真菌和原生生物的生物体中有效地下调目标基因在细胞中的表达的方法和手段。 本发明提供用于下调靶基因的嵌合核酸分子。 本发明还提供了修饰的细胞和生物体，其包含嵌合核酸分子和包含嵌合分子的组合物。
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公开(公告)号：US08183217B2
公开(公告)日：2012-05-22
申请号：US11179504
申请日：2005-07-13
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated, target-specific RNA is provided by transcription of a chimeric gene comprising a promoter and a DNA region encoding the target-specific RNA.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞中目的核酸的表型表达的方法和手段。 优选地，非多腺苷酸化的靶特异性RNA通过包含启动子和编码靶特异性RNA的DNA区的嵌合基因的转录来提供。
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