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公开(公告)号：US5922597A
公开(公告)日：1999-07-13
申请号：US860866
申请日：1997-07-14
IPC分类号： A61K35/12 , C12N5/02 , C12N5/0783 , C12N5/0789 , C12N5/08
CPC分类号： C12N5/0646 , C12N5/0647 , A61K2035/124 , C12N2500/38 , C12N2501/125 , C12N2501/21 , C12N2501/23 , C12N2501/39 , C12N2502/1394
摘要： A method for the culture of hematopoietic cells is provided comprising culturing said cells in stromal conditioned medium comprising one or more cytokines selected from the group consisting of MIP-1.alpha., IL-3, SCF, BB10010 and PF-4, so that the ability of the stem cells to self-replicate and differentiate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
摘要翻译： PCT No.PCT / US96 / 18222 Sec。 371日期1997年7月14日 102（e）日期1997年7月14日PCT 1996年11月13日PCT公布。 出版物WO97 / 18298 日期1997年5月22日提供造血细胞培养方法，包括在基质条件培养基中培养所述细胞，所述基质条件培养基包含一种或多种选自MIP-1α，IL-3，SCF，BB10010和PF-4的细胞因子， 使得维持干细胞自我复制和分化的能力，或保持所确定的祖细胞扩增和分化的能力。
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公开(公告)号：US5460964A
公开(公告)日：1995-10-24
申请号：US32670
申请日：1993-03-17
IPC分类号： C12N5/0789 , C12N5/02 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0647 , C12N2501/21 , C12N2501/23 , C12N2501/39 , C12N2502/1394
摘要： A method for culturing mammalian hematopoietic, preferably stem cells, is provided comprising maintaining a population of human hematopoietic cells in a non-contacting relationship to a population of cultured stromal cells, which populations are in preferably liquid stromal growth medium connection, so that the ability of the stem cells in said population to differentiate and self-replicate is maintained during an extended culture period.
摘要翻译： 提供了一种用于培养哺乳动物造血干细胞的方法，优选干细胞包括维持人体造血细胞群与培养基质细胞群的非接触关系，所述群体优选为液体基质生长培养基连接， 的干细胞在所述群体中进行分化和自我复制是在延长的培养期间保持的。
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公开(公告)号：US5436151A
公开(公告)日：1995-07-25
申请号：US862814
申请日：1992-04-03
IPC分类号： C12N5/0789 , C12N5/02 , C12N5/08
CPC分类号： C12N5/0647 , C12N2501/21 , C12N2501/23 , C12N2501/39 , C12N2502/1394
摘要： A method for culturing mammalian hematopoietic stem cells is provided comprising maintaining a population of human stem cells in fixed, non-contacting relationship to a population of cultured stromal cells, which populations are in liquid stromal growth medium connection, so that the ability of the stem cells to differentiate and self-replicate is maintained during an extended culture period.
摘要翻译： 提供了一种用于培养哺乳动物造血干细胞的方法，其包括将人类干细胞群与培养基质细胞群体固定，非接触的关系保持在一起，所述群体处于液体基质生长培养基连接中，使得茎干能力 在延长的培养期间维持细胞分化和自我复制。
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公开(公告)号：US5605829A
公开(公告)日：1997-02-25
申请号：US346893
申请日：1994-11-30
IPC分类号： C07K14/47 , C07K14/77 , C12N5/0789 , C12N5/00 , A61K38/16 , C07K14/475
CPC分类号： C07K14/4725 , C07K14/77 , C12N5/0647 , C12N2501/125 , C12N2501/22 , C12N2501/2306 , C12N2501/235 , C12N2501/90 , C12N2501/998 , C12N2502/1394
摘要： An anionic macromolecular containing composition is disclosed that is capable of supporting conservation and differentiation of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells. The composition disclosed is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD.
摘要翻译： 公开了一种含阴离子大分子的组合物，其能够支持培养的哺乳动物造血细胞中长期骨髓培养起始细胞的保守和分化。 所公开的组合物是分子量大于200kD的糖蛋白，更特别是蛋白聚糖的混合物。
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5.发明授权
公开(公告)号：US5523286A
公开(公告)日：1996-06-04
申请号：US152051
申请日：1993-11-12
IPC分类号： C07K14/47 , C07K14/77 , C12N5/0789 , A61K38/16 , A61K38/00 , C08B37/08 , C08B37/10 , C12N5/00
CPC分类号： C07K14/4725 , C07K14/77 , C12N5/0647 , C12N2501/125 , C12N2501/22 , C12N2501/2306 , C12N2501/235 , C12N2501/90 , C12N2501/998 , C12N2502/1394
摘要： An anionic macromolecular containing composition is disclosed that is capable of supporting conservation and differentiation of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells. The composition disclosed is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD.
摘要翻译： 公开了一种含阴离子大分子的组合物，其能够支持培养的哺乳动物造血细胞中长期骨髓培养起始细胞的保守和分化。 所公开的组合物是分子量大于200kD的糖蛋白，更特别是蛋白聚糖的混合物。
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