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公开(公告)号:US20060021093A1
公开(公告)日:2006-01-26
申请号:US11185342
申请日:2005-07-20
申请人: Philip Hammer , Todd Hinson , Brian Carr , Nicholas Duck
发明人: Philip Hammer , Todd Hinson , Brian Carr , Nicholas Duck
CPC分类号: C12N9/88 , C12N15/8275
摘要: Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.
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公开(公告)号:US20050204436A1
公开(公告)日:2005-09-15
申请号:US10797270
申请日:2004-03-10
申请人: Philip Hammer , Todd Hinson , Nicholas Duck , Michael Koziel
发明人: Philip Hammer , Todd Hinson , Nicholas Duck , Michael Koziel
CPC分类号: C12N15/8209 , C12N9/1092 , C12N9/88 , C12N15/8274 , C12N15/8275 , C12Y205/01019 , C12Y401/01001 , C12Y401/01005
摘要: Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.
摘要翻译: 提供了赋予对植物细胞和细菌细胞的除草剂抗性的组合物和方法。 所述方法包括用编码除草剂抗性基因的核苷酸序列转化细胞。 特别地,通过与脱羧酶具有同源性的蛋白质的表达赋予除草剂抗性。 组合物包括转化的植物,植物组织和种子,以及转化的细菌细胞。
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公开(公告)号:US20070107078A1
公开(公告)日:2007-05-10
申请号:US11588811
申请日:2006-10-27
申请人: Philip Hammer , Todd Hinson , Nicholas Duck , Michael Koziel
发明人: Philip Hammer , Todd Hinson , Nicholas Duck , Michael Koziel
CPC分类号: C12N15/8274 , C12N9/88 , C12N15/8275
摘要: Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.
摘要翻译: 提供了赋予对植物细胞和细菌细胞的除草剂抗性的组合物和方法。 所述方法包括用编码除草剂抗性基因的核苷酸序列转化细胞。 特别地,通过与脱羧酶具有同源性的蛋白质的表达赋予除草剂抗性。 组合物包括转化的植物,植物组织和种子,以及转化的细菌细胞。
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公开(公告)号:US20060021094A1
公开(公告)日:2006-01-26
申请号:US11185560
申请日:2005-07-20
申请人: Philip Hammer , Todd Hinson , Nicholas Duck
发明人: Philip Hammer , Todd Hinson , Nicholas Duck
CPC分类号: C12N9/88 , C12N15/8275 , C12N15/8278
摘要: Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequences set forth in SEQ ID NOS:1 and 2.
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公开(公告)号:US20110252503A1
公开(公告)日:2011-10-13
申请号:US12916854
申请日:2010-11-01
CPC分类号: C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译: 本发明涉及具有增加的氮利用效率,胁迫耐受性和/或减轻增加产量的限制的转基因植物,或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物 谷氨酸激酶(NAGK)基因,调节植物中的氮利用。 本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征,其改善胁迫耐受性和氮摄取,代谢或两者。 在各种实施方案中,载体构建体包括一个或多个包含SEQ ID NO:1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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公开(公告)号:US08692070B2
公开(公告)日:2014-04-08
申请号:US12916854
申请日:2010-11-01
IPC分类号: C12N15/82 , C12N15/87 , A01H5/00 , A01H5/10 , C12N1/21 , C12N15/00 , C12N15/29 , C12N15/54 , C07H21/04
CPC分类号: C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译: 本发明涉及具有增加的氮利用效率,胁迫耐受性和/或减轻增加产量的限制的转基因植物,或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物 谷氨酸激酶(NAGK)基因,调节植物中的氮利用。 本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征,其改善胁迫耐受性和氮摄取,代谢或两者。 在各种实施方案中,载体构建体包括一个或多个包含SEQ ID NO:1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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公开(公告)号:US20070004907A1
公开(公告)日:2007-01-04
申请号:US11517991
申请日:2006-09-08
申请人: Philip Hammer , Nicholas Duck
发明人: Philip Hammer , Nicholas Duck
IPC分类号: C07K14/415
CPC分类号: C12N15/8275
摘要: Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules encoding glyphosate resistance proteins are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or the nucleotide sequence set forth in SEQ ID NO: 1.
摘要翻译: 提供了赋予对植物,植物细胞,组织和种子的除草剂抗性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的植物,植物细胞,组织和种子。 特别地,提供编码抗草甘膦蛋白质的分离的核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:2所示的氨基酸序列的核苷酸序列或SEQ ID NO:1所示的核苷酸序列的分离的核酸分子。
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公开(公告)号:US20070044175A1
公开(公告)日:2007-02-22
申请号:US11500718
申请日:2006-08-08
申请人: Philip Hammer , Nicholas Duck
发明人: Philip Hammer , Nicholas Duck
CPC分类号: C12N15/8275
摘要: Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules encoding glyphosate resistance proteins are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or the nucleotide sequence set forth in SEQ ID NO:1.
摘要翻译: 提供了赋予对植物,植物细胞,组织和种子的除草剂抗性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的植物,植物细胞,组织和种子。 特别地,提供编码抗草甘膦蛋白质的分离的核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:2所示的氨基酸序列的核苷酸序列或SEQ ID NO:1所示的核苷酸序列的分离的核酸分子。
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9.
公开(公告)号:US20070238646A1
公开(公告)日:2007-10-11
申请号:US11765494
申请日:2007-06-20
申请人: Nadine Carozzi , Tracy Hargiss , Michael Koziel , Nicholas Duck , Brian Carr
发明人: Nadine Carozzi , Tracy Hargiss , Michael Koziel , Nicholas Duck , Brian Carr
CPC分类号: C07H21/04 , C07K14/325
摘要: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof
摘要翻译: 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO:1或3所示的核苷酸序列的分离的核酸分子及其变体
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10.
公开(公告)号:US20070240239A1
公开(公告)日:2007-10-11
申请号:US11765491
申请日:2007-06-20
申请人: Nadine Carozzi , Tracy Hargiss , Michael Koziel , Nicholas Duck , Brian Carr
发明人: Nadine Carozzi , Tracy Hargiss , Michael Koziel , Nicholas Duck , Brian Carr
IPC分类号: C12N15/63 , A01H1/00 , A01H5/00 , A01H7/00 , C12N5/04 , C12N5/14 , C12N15/82 , C07H21/04 , C12N1/20
CPC分类号: C07H21/04 , C07K14/325
摘要: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.
摘要翻译: 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO:1或3所示的核苷酸序列的分离的核酸分子及其变体 。
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