公开(公告)号：US20130296231A1

公开(公告)日：2013-11-07

申请号：US13836855

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn

IPC分类号： A23L1/305

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供带电的营养蛋白质。 在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于 支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

公开(公告)号：US20140342978A1

公开(公告)日：2014-11-20

申请号：US14337563

申请日：2014-07-22

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Michael Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk

IPC分类号： C07K14/00 ,  A23L1/305 ,  A61K38/16

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供带电的营养蛋白质。 在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于 支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

公开(公告)号：US09598474B2

公开(公告)日：2017-03-21

申请号：US14374127

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Rajeev Chillakuru ,  Michael Hamill ,  John F. Kramarczyk

IPC分类号： A23J3/04 ,  C07K14/435 ,  A23L1/305 ,  A61K38/17

CPC分类号： C07K14/435 ,  A23L33/17 ,  A23L33/18 ,  A23V2002/00 ,  A61K38/1709 ,  A23V2200/332 ,  A23V2250/54

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a first polypeptide sequence comprising a fragment of a naturally-occurring nutritive protein. In some embodiments the fragment comprises at least one of a) an enhanced ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) an enhanced ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) an enhanced ratio of essential amino acid residues to total amino acid residues present in the nutritive protein. In some embodiments, the fragment comprises at least one of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein, that is equal to or greater than the corresponding ratio present in a benchmark protein such as whey, egg or soy protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US09605040B2

公开(公告)日：2017-03-28

申请号：US14387675

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： Geoffrey von Maltzahn ,  Michael J. Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk ,  David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver

IPC分类号： C07K14/435 ,  A61K38/00 ,  C07K14/47 ,  A23J3/04 ,  C07K14/415 ,  A23L33/175

CPC分类号： C07K14/415 ,  A23J3/04 ,  A23L33/175 ,  A23V2002/00 ,  A61K38/00 ,  C07K14/47

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US20150232520A1

公开(公告)日：2015-08-20

申请号：US14387677

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： Michael J. Hamill ,  Nathaniel W. Silver ,  Rajeev Chillakuru ,  John F. Kramarczyk ,  David Arthur Berry ,  Brett Adam Boghigian ,  Geoffrey von Maltzahn

IPC分类号： C07K14/47 ,  C07K14/415 ,  C07K14/375

CPC分类号： C07K14/47 ,  A23L33/17 ,  A23V2002/00 ,  A61K38/00 ,  C07K14/375 ,  C07K14/415 ,  C07K14/435 ,  A23V2200/332 ,  A23V2250/54

摘要： Charged nutritive proteins provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 带电营养蛋白质提供。 在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于 支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

公开(公告)号：US20150087602A1

公开(公告)日：2015-03-26

申请号：US14387675

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： Geoffrey von Maltzahn ,  Michael J. Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk ,  David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver

IPC分类号： C07K14/47 ,  C07K14/415

CPC分类号： C07K14/415 ,  A23J3/04 ,  A23L33/175 ,  A23V2002/00 ,  A61K38/00 ,  C07K14/47

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供营养蛋白质。 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于支链氨基酸残基与总氨基酸的比例 存在于乳清蛋白中的酸残基; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于乳清蛋白中存在的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于乳清蛋白中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

公开(公告)号：US08822412B2

公开(公告)日：2014-09-02

申请号：US14219943

申请日：2014-03-19

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Michael Hamill ,  Rajeev Chillakuru

IPC分类号： A23J3/04 ,  C07K14/435 ,  A23L1/305 ,  A23L1/29

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US09611298B2

公开(公告)日：2017-04-04

申请号：US14337563

申请日：2014-07-22

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Michael Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk

IPC分类号： A23J3/04 ,  A61K38/00 ,  C07K14/00 ,  C07K14/415 ,  C12N15/82 ,  A61K38/16 ,  A61K31/198 ,  A23L33/00 ,  A23L33/17 ,  A23L33/18

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US20150057352A1

公开(公告)日：2015-02-26

申请号：US14335071

申请日：2014-07-18

申请人： Pronutria, Inc.

发明人： Subhayu Basu ,  Gaozhong Shen ,  Brett Adam Boghigian ,  David M. Young ,  David Arthur Berry ,  Geoffrey von Maltzahn

IPC分类号： A61K31/201

CPC分类号： A61K31/201 ,  C12N9/0071 ,  C12N9/16 ,  C12P7/6472 ,  C12Y114/19 ,  C12Y114/19001 ,  C12Y114/19006 ,  C12Y301/02014 ,  Y02A50/473

摘要： Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and α-linolenic acid.

摘要翻译： 提供了包含至少18个碳原子的不饱和游离脂肪酸的生产方法。 在一些实施方案中，所述方法包括在培养基中培养工程微生物，其中所述工程化微生物包含至少一种选自酰基 - 脂质去饱和酶δ-9（EC：1.14.19.1），酰基 - 脂质去饱和酶Δ-12 （EC：1.14.19.6），酰基 - 脂肪去饱和酶Δ-15（EC：1.14.19.-）和硫酯酶（EC：3.1.2.14）。 还提供了包含这些重组酶中的至少一种的工程微生物。 所述方法和生物体可用于产生至少一种选自油酸，亚油酸和α-亚麻酸的游离脂肪酸。

公开(公告)号：US08809259B2

公开(公告)日：2014-08-19

申请号：US13836855

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Mike Hamill ,  Rajeev Chillakuru

IPC分类号： A23J3/04 ,  C07K14/435 ,  A23L1/305 ,  C07K14/415 ,  A23J3/00 ,  C12N15/82

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供带电的营养蛋白质。 在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于 支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。