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    Biotin-binding containment systems
    2.
    发明授权
    Biotin-binding containment systems 失效
    生物素结合遏制系统

    公开(公告)号：US5681745A

    公开(公告)日：1997-10-28

    申请号：US432017

    申请日：1995-05-01

    申请人： Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

    发明人： Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

    IPC分类号： C07K14/36 ,  C07K14/465 ,  C12N1/21 ,  C12N15/12 ,  C12N15/70 ,  C12N5/10 ,  C12N1/13

    CPC分类号： C12N15/70 ,  C07K14/36 ,  C07K14/465

    摘要： The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

    摘要翻译： 本发明涉及表达可用于选择性破坏重组细胞如遗传工程微生物的生物素结合成分的遗传容纳系统。 这些系统可以包含链霉亲和素或抗生物素蛋白基因，其表达受可调节启动子控制。 诸如转录效应子的调节剂由另外可能被表达的基因表达，其表达由遏制系统控制。 试剂的表达可以设计成响应环境中的生理变化。 本发明还涉及用于选择性检测或跟踪重组细胞和含有这些遗传包涵体系的真核和原核细胞的遏制系统和方法。

    Process of making Langmuir-Blodgett films having photo-electronic properties
    3.
    发明授权
    Process of making Langmuir-Blodgett films having photo-electronic properties 失效
    制作具有光电特性的Langmuir-Blodgett薄膜的工艺

    公开(公告)号：US5260004A

    公开(公告)日：1993-11-09

    申请号：US802675

    申请日：1991-12-02

    申请人： Lynne A. Samuelson ,  David L. Kaplan ,  Kenneth A. Marx ,  Sukant K. Tripathy ,  Jayant Kumar

    发明人： Lynne A. Samuelson ,  David L. Kaplan ,  Kenneth A. Marx ,  Sukant K. Tripathy ,  Jayant Kumar

    IPC分类号： B05D1/20 ,  B29C41/12 ,  B29C67/24 ,  B29C41/02 ,  B29C71/02 ,  B29C71/04

    CPC分类号： B82Y30/00 ,  B05D1/202 ,  B29C41/12 ,  B29C67/24 ,  B82Y40/00 ,  B29K2089/00

    摘要： Langmuir-Blodgett films having photo-electronic properties and methods of making the same. The instant films may be made, for example, by spreading a mixture of one or more types of biotinylated lipids and one or more types of electrically-conductive lipids over a water-miscible liquid subphase. Conjugated molecules comprising a biotin-binding component made up of an avidin or streptavidin molecule or a fragment or derivative thereof having biotin-binding activity and a photodynamic proteinaceous component are then injected into the subphase. Because of the affinity between biotin and the biotin-binding component, the conjugated molecules bind to the biotinylated lipids. The air-subphase interface is then compressed, causing the biotinylated lipids and electrically-conductive lipids to form a monolayer thereat. In one embodiment, the biotin-binding component has biotin-binding sites available on its underside which may be used to bind biotinylated derivatives of one or more different species of photodynamic proteinaceous components and/or to build up a multilayered complex of biotin-binding molecules and functional proteinaceous components using biotinylated rigid or flexible couplers.

    摘要翻译： 具有光电特性的Langmuir-Blodgett薄膜及其制造方法。 可以例如通过将一种或多种类型的生物素化脂质和一种或多种类型的导电脂质的混合物铺在水混溶液体相上来制备即时膜。 然后将包含由具有生物素结合活性的抗生物素蛋白或链霉抗生物素蛋白分子或其片段或衍生物的生物素结合组分的结合分子和光动力学蛋白质组分注入到亚相中。 由于生物素与生物素结合成分之间的亲和力，共轭分子与生物素化的脂质结合。 然后将空气 - 亚相界面压缩，导致生物素化的脂质和导电脂质在其上形成单层。 在一个实施方案中，生物素结合组分在其下侧具有可用的生物素结合位点，其可用于结合一种或多种不同种类的光动力蛋白质组分的生物素化衍生物和/或建立生物素结合分子的多层复合物 和使用生物素化的刚性或柔性耦合剂的功能蛋白质组分。

    Photodynamic protein-based photodetector and photodetector system for image detection and processing
    4.
    发明授权
    Photodynamic protein-based photodetector and photodetector system for image detection and processing 失效
    光动力蛋白质光电探测器和光电探测器系统，用于图像检测和处理

    公开(公告)号：US5438192A

    公开(公告)日：1995-08-01

    申请号：US166398

    申请日：1993-12-09

    申请人： David L. Kaplan ,  Lynne A. Samuelson ,  Bonnie J. Wiley ,  Kenneth A. Marx ,  Jayant Kumar ,  Sukant K. Tripathy ,  Sandip K. Sengupta ,  Mario J. Cazeca

    发明人： David L. Kaplan ,  Lynne A. Samuelson ,  Bonnie J. Wiley ,  Kenneth A. Marx ,  Jayant Kumar ,  Sukant K. Tripathy ,  Sandip K. Sengupta ,  Mario J. Cazeca

    IPC分类号： G01J1/42 ,  H01L31/08 ,  H01L51/30 ,  H01J40/14

    CPC分类号： H01L51/42 ,  B82Y10/00 ,  H01L51/0093 ,  H01L27/307 ,  Y02E10/549 ,  Y10S428/919

    摘要： A photodetection device uses configurations of photodynamic proteins which exhibit a change in electrical conductivity in response to a corresponding change in incident light intensity in the presence of an applied voltage. The photodynamic proteins are coupled to an electrical conductor, a voltage source and a conductivity sensor. The photodynamic protein complex includes at least one layer of a photodynamic protein and preferably includes a multi-layered thin-film structure with each layer comprised of either a photodynamic protein or a conductive polymer or oligomer. Groups of linked photodetectors where the photodetectors have different, but overlapping, spectral response ranges are used to detect specific wavelengths of incident light. An array of these groups of linked photodetectors arranged in a predetermined spatial pattern allows detection of both colon and images. An image processing system is constructed from this array of groups of linked photodetectors by coupling the output of the array to a processing component and the output of the processing component to an output device. A dynamic adaptive camouflage system is derived from the image processing system by mounting the photodetectors and display devices on an apparatus to be camouflaged and displaying a spatially shifted image of the incident ambient light.

    摘要翻译： 光电检测装置使用光动力学蛋白质的配置，其响应于存在施加电压的入射光强度的相应变化而呈现导电性变化。 光动力学蛋白质耦合到电导体，电压源和电导率传感器。 光动力学蛋白质复合物包括至少一层光动力学蛋白质，并且优选地包括具有由光动力学蛋白质或导电聚合物或低聚物组成的每层的多层薄膜结构。 光电探测器具有不同但重叠的光谱响应范围的联接光电检测器组被用于检测入射光的特定波长。 以预定的空间图案布置的这些组的相连的光电探测器的阵列允许检测结肠和图像。 通过将阵列的输出耦合到处理部件和将处理部件的输出耦合到输出装置，从这组连接的光电检测器组构成图像处理系统。 通过将光电检测器和显示装置安装在要伪装的装置上并显示入射环境光的空间位移图像，从图像处理系统得到动态适应伪装系统。

    Chemiluminescence-based method for rapid and sensitive in-situ detection of organophosphorus compounds and metal ions
    5.
    发明授权
    Chemiluminescence-based method for rapid and sensitive in-situ detection of organophosphorus compounds and metal ions 失效
    基于化学发光的方法，用于快速和灵敏地原位检测有机磷化合物和金属离子

    公开(公告)号：US5846753A

    公开(公告)日：1998-12-08

    申请号：US445565

    申请日：1995-05-30

    申请人： Joseph A. Akkara ,  David L. Kaplan ,  Madhu S. R. Ayyagari ,  Kenneth A. Marx ,  Sanjay Kamtekar ,  Rajiv Pande ,  Sukant K. Tripathy ,  Jayant Kumar

    发明人： Joseph A. Akkara ,  David L. Kaplan ,  Madhu S. R. Ayyagari ,  Kenneth A. Marx ,  Sanjay Kamtekar ,  Rajiv Pande ,  Sukant K. Tripathy ,  Jayant Kumar

    IPC分类号： C12Q1/42 ,  C07D305/14 ,  C12Q1/44

    CPC分类号： C12Q1/42 ,  C12Q2334/00

    摘要： A method of detecting the presence of a substance being monitored in a medium, selected from the group of substances including organophosphorus compounds and the metal ions Zn, Be and Bi, including the steps of: providing a 1,2-dioxetane phenyl phosphate compound; providing a phosphatase that catalytically degrades the 1,2-dioxetane phenyl phosphate compound to produce light, the catalytic activity of the phosphatase toward 1,2-dioxetane phenyl phosphate compound being altered by the substance being monitored; exposing the 1,2-dioxetane phenyl phosphate compound and the phosphatase together to a medium which may contain the substance being monitored; detecting light produced after the exposing step; and determining, from the detected light, the presence and concentration in the medium of the substance being monitored.

    摘要翻译： 一种检测在包括有机磷化合物和金属离子Zn，Be和Bi的物质组中的介质中被监测物质的存在的方法，包括以下步骤：提供1,2-二氧环乙烷苯基磷酸酯化合物; 提供催化降解1,2-二氧杂环丁烷苯基磷酸酯化合物以产生光的磷酸酶，磷酸酯对正在监测的物质的1,2-二氧环乙烷苯基磷酸酯化合物的催化活性改变; 将1,2-二氧环乙烷苯基磷酸酯化合物和磷酸酶一起暴露于可能含有被监测物质的介质中; 检测曝光步骤后产生的光; 并且从检测到的光中确定被监测物质的介质中的存在和浓度。

    Chiral Metamaterials
    7.
    发明申请
    Chiral Metamaterials 有权
    手性超材料

    公开(公告)号：US20100141358A1

    公开(公告)日：2010-06-10

    申请号：US11901964

    申请日：2007-09-19

    申请人： Alkim Akyurtlu ,  Kenneth A. Marx ,  Nantakan Wongkasem

    发明人： Alkim Akyurtlu ,  Kenneth A. Marx ,  Nantakan Wongkasem

    IPC分类号： H01P7/10 ,  H01F7/06 ,  H05K3/30

    CPC分类号： H01P1/20 ,  Y10T29/4902 ,  Y10T29/4913

    摘要： A metamaterial includes a dielectric substrate and an array of discrete resonators at the dielectric substrate, wherein each of the discrete resonators has a shape that is independently selected from: an F-type shape; an E-type shape; or a y-type shape. A parameter of a chiral metamaterial is determined and a chiral metamaterial having such a parameter is prepared by the use of a model of the chiral metamaterial. The metamaterial model includes an array of discrete resonators. In one embodiment, each of the discrete resonators has a shape that is independently selected from the group consisting of: an F-type shape; an E-type shape; and a y-type shape. To the metamaterial model, electromagnetic (EM) radiation, preferably plane-polarized EM radiation in a visible, ultraviolet or near-infrared region, having at least one wavelength that is larger than the largest dimension of at least resonator of the metamaterial model, is applied. Varying at least one characteristic of the metamaterial model and/or at least one wavelength of the applied EM radiation modulates EM interaction of the applied EM radiation with the metamaterial model, thereby determining a parameter of the chiral metamaterial. By the use of a model of the chiral metamaterial, a number of discrete resonators of a chiral metamaterial that are arrayed in a direction perpendicular to a propagation axis of EM radiation is also determined.

    摘要翻译： 超材料包括电介质基板和电介质基板上的离散谐振器阵列，其中每个离散谐振器具有独立地选自以下的形状：F型形状; E型形状; 或y型形状。 确定手性超材料的参数，并通过使用手性超材料的模型制备具有这种参数的手性超材料。 超材料模型包括离散谐振器阵列。 在一个实施例中，每个离散谐振器具有独立地选自以下的形状：F型形状; E型形状; 和y型形状。 对于超材料模型，电磁（EM）辐射，优选具有至少一个波长的至少一个波长的可见光，紫外或近红外区域中的平面极化EM辐射，其至少超过至少是超材料模型的谐振器的最大尺寸， 应用。 改变超材料模型的至少一个特征和/或所施加的EM辐射的至少一个波长调制所施加的EM辐射与超材料模型的EM相互作用，从而确定手性超材料的参数。 通过使用手性超材料的模型，还确定了沿与EM辐射的传播轴垂直的方向排列的许多手性超材料的离散谐振器。

    Chiral metamaterials
    9.
    发明授权
    Chiral metamaterials 有权
    手性超材料

    公开(公告)号：US08271241B2

    公开(公告)日：2012-09-18

    申请号：US11901964

    申请日：2007-09-19

    申请人： Alkim Akyurtlu ,  Kenneth A. Marx ,  Nantakan Wongkasem

    发明人： Alkim Akyurtlu ,  Kenneth A. Marx ,  Nantakan Wongkasem

    IPC分类号： G06G7/48

    CPC分类号： H01P1/20 ,  Y10T29/4902 ,  Y10T29/4913

    摘要： A metamaterial includes a dielectric substrate and an array of discrete resonators at the dielectric substrate, wherein each of the discrete resonators has a shape that is independently selected from: an F-type shape; an E-type shape; or a y-type shape. A parameter of a chiral metamaterial is determined and a chiral metamaterial having such a parameter is prepared by the use of a model of the chiral metamaterial. The metamaterial model includes an array of discrete resonators. In one embodiment, each of the discrete resonators has a shape that is independently selected from the group consisting of: an F-type shape; an E-type shape; and a y-type shape. To the metamaterial model, electromagnetic (EM) radiation, preferably plane-polarized EM radiation in a visible, ultraviolet or near-infrared region, having at least one wavelength that is larger than the largest dimension of at least resonator of the metamaterial model, is applied. Varying at least one characteristic of the metamaterial model and/or at least one wavelength of the applied EM radiation modulates EM interaction of the applied EM radiation with the metamaterial model, thereby determining a parameter of the chiral metamaterial. By the use of a model of the chiral metamaterial, a number of discrete resonators of a chiral metamaterial that are arrayed in a direction perpendicular to a propagation axis of EM radiation is also determined.

    摘要翻译： 超材料包括电介质基板和电介质基板上的离散谐振器阵列，其中每个离散谐振器具有独立地选自以下的形状：F型形状; E型形状; 或y型形状。 确定手性超材料的参数，并通过使用手性超材料的模型制备具有这种参数的手性超材料。 超材料模型包括离散谐振器阵列。 在一个实施例中，每个离散谐振器具有独立地选自以下的形状：F型形状; E型形状; 和y型形状。 对于超材料模型，电磁（EM）辐射，优选具有至少一个波长的至少一个波长的可见光，紫外或近红外区域中的平面极化EM辐射，其至少超过至少是超材料模型的谐振器的最大尺寸， 应用。 改变超材料模型的至少一个特征和/或所施加的EM辐射的至少一个波长调制所施加的EM辐射与超材料模型的EM相互作用，从而确定手性超材料的参数。 通过使用手性超材料的模型，还确定了沿与EM辐射的传播轴垂直的方向排列的许多手性超材料的离散谐振器。

    ACOUSTIC WAVE (AW) SENSING DEVICES USING LIVE CELLS
    10.
    发明申请
    ACOUSTIC WAVE (AW) SENSING DEVICES USING LIVE CELLS 审中-公开
    ACOUSTIC WAVE（AW）感应装置使用活细胞

    公开(公告)号：US20120258444A1

    公开(公告)日：2012-10-11

    申请号：US13300407

    申请日：2011-11-18

    申请人： Joel M. Therrien ,  Susan J. Braunhut ,  Kenneth A. Marx ,  Gang Wang ,  Malavika Vashist ,  Abiche H. Dewilde ,  Jianping Zhang ,  Hongwei Sun ,  Lian Dai ,  Sai Liu

    发明人： Joel M. Therrien ,  Susan J. Braunhut ,  Kenneth A. Marx ,  Gang Wang ,  Malavika Vashist ,  Abiche H. Dewilde ,  Jianping Zhang ,  Hongwei Sun ,  Lian Dai ,  Sai Liu

    IPC分类号： G01N29/12 ,  C12M1/34

    CPC分类号： G01N29/14 ,  G01N29/036 ,  G01N29/12 ,  G01N2291/014 ,  G01N2291/02466

    摘要： In one embodiment according to the invention, there is provided a method of sensing a response of a living cell or virus to a change in conditions. The method comprises applying an essentially constant external electromotive force that causes oscillation of an acoustic wave device at essentially constant amplitude and frequency under steady state conditions. The acoustic wave device has attached at least one living cell or virus. A combined oscillating system including the acoustic wave device and the living cell or virus exhibits a fundamental frequency and at least one harmonic frequency of the combined oscillating system. The living cell or virus is exposed to a change in an environmental condition while oscillating the combined oscillating system under the essentially constant external electromotive force, whereby a response of the living cell or virus to the change in environmental condition will be indicated by a change in at least one of frequency and amplitude of the oscillation of at least one harmonic frequency of the combined oscillating system.

    摘要翻译： 在根据本发明的一个实施例中，提供了一种感测活细胞或病毒对病情变化的反应的方法。 该方法包括施加基本上恒定的外部电动势，其在稳定状态条件下使声波器件的振荡基本恒定在幅度和频率上。 声波装置附着有至少一个活细胞或病毒。 包括声波装置和活细胞或病毒的组合振荡系统表现出组合的振荡系统的基频和至少一个谐波频率。 活体细胞或病毒暴露于环境状态的变化，同时在基本上恒定的外部电动势下振荡组合的振荡系统，由此活细胞或病毒对环境条件变化的反应将通过 组合振荡系统的至少一个谐波频率的振荡的频率和振幅中的至少一个。